Abstract
Summary Symbiotic nitrogen (N 2 ) fixation plays a vital role in sustainable agriculture. Efficient N 2 fixation requires various materials, including phosphate (Pi); however, the molecular mechanism underlying the transport of Pi into nodules and bacteroids remains largely unknown. A nodule‐localized Pi transporter, Gm PT 7, was functionally characterized in soybean ( Glycine max ) and its role in N 2 fixation and yield was investigated via composite and whole transgenic plants. Gm PT 7 protein was localized to the plasma membrane and showed transport activity for Pi in yeast. Altered expression of Gm PT 7 changed 33 Pi uptake from rhizosphere and translocation to bacteroids. Gm PT 7 was mainly localized to the outer cortex and fixation zones of the nodules. Overexpression of Gm PT 7 promoted nodulation, and increased plant biomass, shoot nitrogen and phosphorus content, resulting in improved soybean yield by up to 36%. Double suppression of Gm PT 5 and Gm PT 7 led to nearly complete elimination of nodulation and over 50% reduction in plant biomass, shoot nitrogen and phosphorus content, indicating that both Gm PT 7 and Gm PT 5 contribute to Pi transport for N 2 fixation. Taken together, our results indicate that Gm PT 7 is a transporter responsible for direct Pi entry to nodules and further to fixation zones, which is required for enhancing symbiotic N 2 fixation and grain yield of soybean.
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Claims 11
Claim · model-written summary
GmPT7 protein is localized to the plasma membrane.
- Taxon
- Glycine max
Glycine maxe1 - Gene
- Gm PT 7e2
- Outcome
- localized to the plasma membranee2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“functionally characterized in soybean ( Glycine max )”
e2“Gm PT 7 protein was localized to the plasma membrane and showed transport activity for Pi in yeast”
Claim · model-written summary
GmPT7 protein showed transport activity for Pi in yeast.
- Taxon
- Glycine maxGlycine maxe1
- Gene
- Gm PT 7e2
- Outcome
- transport activity for Pie2
- Qualifier
- in yeaste2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“functionally characterized in soybean ( Glycine max )”
e2“Gm PT 7 protein was localized to the plasma membrane and showed transport activity for Pi in yeast”
Claim · model-written summary
Altered GmPT7 expression changed Pi uptake from the rhizosphere and translocation to bacteroids.
- Taxon
- Glycine maxGlycine maxe1
- Treatment
- Altered expression of Gm PT 7e3
- Interactor
- bacteroidse3symbiont
- Gene
- Gm PT 7e3
- Outcome
- Pi uptake from rhizosphere and translocation to bacteroidse3
- Direction
- changede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“functionally characterized in soybean ( Glycine max )”
e3“Altered expression of Gm PT 7 changed 33 Pi uptake from rhizosphere and translocation to bacteroids”
Claim · model-written summary
GmPT7 was localized mainly to the outer cortex and fixation zones of nodules.
- Taxon
- Glycine maxGlycine maxe1
- Organ / stage
- outer cortex and fixation zones of the nodulese4
- Gene
- Gm PT 7e4
- Outcome
- localizede4
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“functionally characterized in soybean ( Glycine max )”
e4“Gm PT 7 was mainly localized to the outer cortex and fixation zones of the nodules”
Claim · model-written summary
Overexpression of GmPT7 promoted nodulation.
- Taxon
- Glycine maxGlycine maxe1
- Treatment
- Overexpression of Gm PT 7e5
- Outcome
- nodulatione5
- Direction
- promotede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“functionally characterized in soybean ( Glycine max )”
e5“Overexpression of Gm PT 7 promoted nodulation, and increased plant biomass, shoot nitrogen and phosphorus content, resulting in improved soybean yield by up to 36%”
Claim · model-written summary
Overexpression of GmPT7 increased plant biomass, shoot nitrogen and phosphorus content.
- Taxon
- Glycine maxGlycine maxe1
- Treatment
- Overexpression of Gm PT 7e5
- Outcome
- plant biomass, shoot nitrogen and phosphorus contente5
- Direction
- increasede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“functionally characterized in soybean ( Glycine max )”
e5“Overexpression of Gm PT 7 promoted nodulation, and increased plant biomass, shoot nitrogen and phosphorus content, resulting in improved soybean yield by up to 36%”
Claim · model-written summary
Overexpression of GmPT7 improved soybean yield by up to 36%.
- Taxon
- Glycine maxGlycine maxe1
- Treatment
- Overexpression of Gm PT 7e5
- Outcome
- soybean yielde5
- Direction
- improvede5
- Value
- 36%e5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“functionally characterized in soybean ( Glycine max )”
e5“Overexpression of Gm PT 7 promoted nodulation, and increased plant biomass, shoot nitrogen and phosphorus content, resulting in improved soybean yield by up to 36%”
Claim · model-written summary
Double suppression of GmPT5 and GmPT7 nearly eliminated nodulation.
- Taxon
- Glycine maxGlycine maxe1
- Treatment
- Double suppression of Gm PT 5 and Gm PT 7e6
- Gene
- Gm PT 5 and Gm PT 7e6
- Outcome
- nodulatione6
- Direction
- eliminatione6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“functionally characterized in soybean ( Glycine max )”
e6“Double suppression of Gm PT 5 and Gm PT 7 led to nearly complete elimination of nodulation and over 50% reduction in plant biomass, shoot nitrogen and phosphorus content”
Claim · model-written summary
Double suppression of GmPT5 and GmPT7 reduced plant biomass, shoot nitrogen and phosphorus content by over 50%.
- Taxon
- Glycine maxGlycine maxe1
- Treatment
- Double suppression of Gm PT 5 and Gm PT 7e6
- Gene
- Gm PT 5 and Gm PT 7e6
- Outcome
- plant biomass, shoot nitrogen and phosphorus contente6
- Direction
- reductione6
- Value
- over 50%e6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“functionally characterized in soybean ( Glycine max )”
e6“Double suppression of Gm PT 5 and Gm PT 7 led to nearly complete elimination of nodulation and over 50% reduction in plant biomass, shoot nitrogen and phosphorus content”
Claim · model-written summary
GmPT7 and GmPT5 both contribute to Pi transport for N2 fixation.
- Taxon
- Glycine maxGlycine maxe1
- Gene
- Gm PT 7 and Gm PT 5e7
- Outcome
- Pi transport for N 2 fixatione7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“functionally characterized in soybean ( Glycine max )”
e7“indicating that both Gm PT 7 and Gm PT 5 contribute to Pi transport for N 2 fixation”
Claim · model-written summary
GmPT7 is a transporter responsible for direct Pi entry to nodules, required for enhancing symbiotic N2 fixation and grain yield.
- Taxon
- Glycine maxGlycine maxe1
- Gene
- Gm PT 7e0
- Outcome
- enhancing symbiotic N 2 fixation and grain yielde0
- Qualifier
- direct Pi entry to nodulese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“functionally characterized in soybean ( Glycine max )”
e0“Gm PT 7 is a transporter responsible for direct Pi entry to nodules and further to fixation zones, which is required for enhancing symbiotic N 2 fixation and grain yield of soybean”
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