Abstract
Grafting has become a common practice among tomato growers to obtain vigorous plants. These plants present a substantial increase in nitrogen (N) uptake from the root zone. However, the mechanisms involved in this higher uptake capacity have not been investigated. To elucidate whether the increase in N uptake in grafted tomato plants under high N demand conditions is related to the functioning of low- (high capacity) or high-affinity (low capacity) root plasma membrane transporters, a series of experiments were conducted. Plants grafted onto a vigorous rootstock, as well as ungrafted and homograft plants, were exposed to two radiation levels (400 and 800 µmol m−2 s−1). We assessed root plasma membrane nitrate transporters (LeNRT1.1, LeNRT1.2, LeNRT2.1, LeNRT2.2 and LeNRT2.3) expression, Michaelis‒Menten kinetics parameters (Vmax and Km), root and leaf nitrate reductase activity, and root respiration rates. The majority of nitrate uptake is mediated by LeNRT1.1 and LeNRT1.2 in grafted and ungrafted plants. Under high N demand conditions, vigorous rootstocks show similar levels of expression for LeNRT1.1 and LeNRT1.2, whereas ungrafted plants present a higher expression of LeNRT1.2. No differences in the uptake capacity (evaluated as Vmax), root respiration rates, or root nitrate assimilation capacity were found among treatments.
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Claims 4
Claim · model-written summary
LeNRT1.1 and LeNRT1.2 mediate the majority of nitrate uptake in grafted and ungrafted tomato plants.
- Taxon
- tomato
Solanum lycopersicumt0 - Gene
- LeNRT1.1 and LeNRT1.2e1
- Outcome
- nitrate uptakee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“LeNRT1.1 Improves Nitrate Uptake in Grafted Tomato Plants under High Nitrogen Demand”
e1“The majority of nitrate uptake is mediated by LeNRT1.1 and LeNRT1.2 in grafted and ungrafted plants”
Claim · model-written summary
Under high N demand, vigorous rootstocks showed similar LeNRT1.1 and LeNRT1.2 expression levels compared with ungrafted plants.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- vigorous rootstockse2
- Gene
- LeNRT1.1 and LeNRT1.2e2
- Outcome
- expressione2
- Direction
- similar levelse2
- Comparator
- ungrafted plantse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“LeNRT1.1 Improves Nitrate Uptake in Grafted Tomato Plants under High Nitrogen Demand”
e2“Under high N demand conditions, vigorous rootstocks show similar levels of expression for LeNRT1.1 and LeNRT1.2, whereas ungrafted plants present a higher expression of LeNRT1.2”
Claim · model-written summary
Ungrafted tomato plants showed higher LeNRT1.2 expression than vigorous rootstocks under high N demand.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- ungrafted plantse2
- Gene
- LeNRT1.2e2
- Outcome
- expressione2
- Direction
- highere2
- Comparator
- vigorous rootstockse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“LeNRT1.1 Improves Nitrate Uptake in Grafted Tomato Plants under High Nitrogen Demand”
e2“Under high N demand conditions, vigorous rootstocks show similar levels of expression for LeNRT1.1 and LeNRT1.2, whereas ungrafted plants present a higher expression of LeNRT1.2”
Claim · model-written summary
Nitrate transporter uptake capacity, root respiration and root nitrate assimilation did not differ among tomato treatments.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- treatmentse3
- Outcome
- uptake capacity (evaluated as Vmax), root respiration rates, or root nitrate assimilation capacitye3
- Direction
- no_changeinferrede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“LeNRT1.1 Improves Nitrate Uptake in Grafted Tomato Plants under High Nitrogen Demand”
e3“No differences in the uptake capacity (evaluated as Vmax), root respiration rates, or root nitrate assimilation capacity were found among treatments”
The same questions in other papers
grafting · molecular: expression · Solanum lycopersicum (tomato)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.