Abstract
Damage and degradation of cellular proteins is observed during seed deterioration due to aging. L-isoaspartyl methyltransferase (EC 2.1.1.77) is an enzyme hypothesized to play a role in limiting and repairing aging-induced damage of proteins. Tomato seeds ( Lycopersicon esculentum `New Yorker') were assayed for changes in L-isoaspartyl methyltransferase activity during accelerated aging and after osmotic priming. Accelerated aging of seeds for 1 to 4 days at 45°C and 100% humidity reduced germination from 94% to 71%, the mean time of germination (MTG) increased from 2.4 to 5.8 days and was accompanied by a correlative decrease in L-isoaspartyl methyltransferase activity R 2 = 0.90. Aged and untreated seeds were primed for 7 days at 20°C in darkness using aerated solutions of 3% KNO 3 or polyethylene glycol 8000 (PEG) with equivalent osmotic potential (–1.25 MPa). Priming with KNO 3 decreased the MTG but not germination percentage for untreated seeds. Priming did not affect L-isoaspartyl methyltransferase activity in untreated seeds but restored activity in aged seeds primed in KNO 3 to levels near that of untreated seeds. Priming with PEG did not effectively improve the MTG or increase L-isoaspartyl methyltransferase activity. During germination, enzyme activity remained constant for 48 hours post-imbibition and then declined suggesting the enzyme was developmentally regulated and inactivated or degraded as radicle emergence occurred.
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Claims 10
Claim · model-written summary
Accelerated aging reduced tomato seed germination from 94% to 71%.
- Taxon
- Tomato
Solanum lycopersicumt0 - Treatment
- Accelerated aging of seeds for 1 to 4 days at 45°C and 100% humiditye0
- Outcome
- germinatione0
- Direction
- reducede0
- Value
- 94% to 71%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Accelerated aging of seeds for 1 to 4 days at 45°C and 100% humidity reduced germination from 94% to 71%”
t0“Tomato Seed”
Claim · model-written summary
Accelerated aging increased mean germination time from 2.4 to 5.8 days.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- Accelerated aginge1
- Outcome
- mean time of germination (MTG)e0
- Direction
- increasede0
- Value
- 2.4 to 5.8 dayse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the mean time of germination (MTG) increased from 2.4 to 5.8 days”
t0“Tomato Seed”
e1“Accelerated aging of seeds for 1 to 4 days at 45°C and 100% humidity”
Claim · model-written summary
Aging correlated with lower L-isoaspartyl methyltransferase activity.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- Accelerated aginge1
- Outcome
- L-isoaspartyl methyltransferase activitye0
- Direction
- decreasee0
- Value
- R 2 = 0.90e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was accompanied by a correlative decrease in L-isoaspartyl methyltransferase activity R 2 = 0.90”
e1“Accelerated aging of seeds for 1 to 4 days at 45°C and 100% humidity”
t0“Tomato Seed”
Claim · model-written summary
KNO 3 priming decreased mean germination time in untreated seeds.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- Priming with KNO 3e0
- Outcome
- MTGe0
- Direction
- decreasede0
- Comparator
- untreated seedse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Priming with KNO 3 decreased the MTG but not germination percentage for untreated seeds”
t0“Tomato Seed”
Claim · model-written summary
KNO 3 priming did not change germination percentage in untreated seeds.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- Priming with KNO 3e0
- Outcome
- germination percentagee0
- Direction
- note0
- Comparator
- untreated seedse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Priming with KNO 3 decreased the MTG but not germination percentage for untreated seeds”
t0“Tomato Seed”
Claim · model-written summary
Priming did not affect methyltransferase activity in untreated seeds.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- Priminge0
- Outcome
- L-isoaspartyl methyltransferase activitye0
- Direction
- did not affecte0
- Comparator
- untreated seedse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Priming did not affect L-isoaspartyl methyltransferase activity in untreated seeds”
t0“Tomato Seed”
Claim · model-written summary
KNO 3 priming restored methyltransferase activity in aged seeds near untreated levels.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- primed in KNO 3e0
- Outcome
- activitye0
- Direction
- restorede0
- Comparator
- untreated seedse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“restored activity in aged seeds primed in KNO 3 to levels near that of untreated seeds”
t0“Tomato Seed”
Claim · model-written summary
PEG priming did not effectively improve mean germination time.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- Priming with PEGe0
- Outcome
- MTGe0
- Direction
- did not effectively improvee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Priming with PEG did not effectively improve the MTG or increase L-isoaspartyl methyltransferase activity”
t0“Tomato Seed”
Claim · model-written summary
PEG priming did not increase methyltransferase activity.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- Priming with PEGe0
- Outcome
- L-isoaspartyl methyltransferase activitye0
- Direction
- did not effectively improvee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Priming with PEG did not effectively improve the MTG or increase L-isoaspartyl methyltransferase activity”
t0“Tomato Seed”
Claim · model-written summary
Methyltransferase activity stayed constant for 48 hours then declined during germination.
- Taxon
- TomatoSolanum lycopersicumt0
- Outcome
- enzyme activitye0
- Direction
- remained constant for 48 hours post-imbibition and then declinede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“During germination, enzyme activity remained constant for 48 hours post-imbibition and then declined”
t0“Tomato Seed”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.