Abstract
HaHB4 is a sunflower transcription factor belonging to the homeodomain-leucine zipper I family whose ectopic expression in Arabidopsis triggers drought tolerance. The use of PCR to clone the HaHB4 coding sequence for wheat transformation caused unprogrammed mutations producing subtle differences in its activation ability in yeast. Transgenic wheat plants carrying a mutated version of HaHB4 were tested in 37 field experiments. A selected transgenic line yielded 6% more (P<0.001) and had 9.4% larger water use efficiency (P<0.02) than its control across the evaluated environments. Differences in grain yield between cultivars were explained by the 8% improvement in grain number per square meter (P<0.0001), and were more pronounced in stress (16% benefit) than in non-stress conditions (3% benefit), reaching a maximum of 97% in one of the driest environments. Increased grain number per square meter of transgenic plants was accompanied by positive trends in spikelet numbers per spike, tillers per plant, and fertile florets per plant. The gene transcripts associated with abiotic stress showed that HaHB4's action was not dependent on the response triggered either by RD19 or by DREB1a, traditional candidates related to water deficit responses. HaHB4 enabled wheat to show some of the benefits of a species highly adapted to water scarcity, especially in marginal regions characterized by frequent droughts.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
PCR cloning caused mutations in HaHB4 that altered yeast activation ability.
- Taxon
- wheat
Triticum aestivume0 - Gene
- HaHB4e0
- Outcome
- subtle differences in its activation ability in yeaste0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The use of PCR to clone the HaHB4 coding sequence for wheat transformation caused unprogrammed mutations producing subtle differences in its activation ability in yeast.”
Claim · model-written summary
The selected transgenic wheat line yielded 6% more than control across field environments.
- Taxon
- wheatTriticum aestivume1
- Material
- selected transgenic linee0
- Treatment
- Transgenic wheat plants carrying a mutated version of HaHB4e1
- Gene
- HaHB4e1
- Outcome
- yieldede0
- Direction
- moree0
- Value
- 6%e0
- Comparator
- its controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A selected transgenic line yielded 6% more (P<0.001) and had 9.4% larger water use efficiency (P<0.02) than its control across the evaluated environments.”
e1“Transgenic wheat plants carrying a mutated version of HaHB4”
Claim · model-written summary
The selected line had 9.4% greater water use efficiency than control.
- Taxon
- wheatTriticum aestivume1
- Treatment
- Transgenic wheat plants carrying a mutated version of HaHB4e1
- Gene
- HaHB4e1
- Outcome
- water use efficiencye0
- Direction
- largere0
- Value
- 9.4%e0
- Comparator
- its controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A selected transgenic line yielded 6% more (P<0.001) and had 9.4% larger water use efficiency (P<0.02) than its control across the evaluated environments.”
e1“Transgenic wheat plants carrying a mutated version of HaHB4”
Claim · model-written summary
Grain number per square meter improved by 8%.
- Taxon
- wheatTriticum aestivume1
- Treatment
- Transgenic wheat plants carrying a mutated version of HaHB4e1
- Outcome
- grain number per square metere0
- Direction
- improvemente0
- Value
- 8%e0
- Comparator
- cultivarse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Differences in grain yield between cultivars were explained by the 8% improvement in grain number per square meter (P<0.0001)”
e1“Transgenic wheat plants carrying a mutated version of HaHB4”
Claim · model-written summary
Yield benefit was 16% under stress.
- Taxon
- wheatTriticum aestivume1
- Treatment
- Transgenic wheat plants carrying a mutated version of HaHB4e1
- Outcome
- benefite0
- Direction
- more pronouncede0
- Value
- 16%e0
- Qualifier
- stresse0
- Comparator
- non-stress conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“more pronounced in stress (16% benefit) than in non-stress conditions (3% benefit)”
e1“Transgenic wheat plants carrying a mutated version of HaHB4”
Claim · model-written summary
Yield benefit was 3% under non-stress conditions.
- Taxon
- wheatTriticum aestivume1
- Treatment
- Transgenic wheat plants carrying a mutated version of HaHB4e1
- Outcome
- benefite0
- Direction
- benefite0
- Value
- 3%e0
- Qualifier
- non-stress conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stress (16% benefit) than in non-stress conditions (3% benefit)”
e1“Transgenic wheat plants carrying a mutated version of HaHB4”
Claim · model-written summary
Yield benefit reached 97% in one of the driest environments.
- Taxon
- wheatTriticum aestivume1
- Treatment
- Transgenic wheat plants carrying a mutated version of HaHB4e1
- Outcome
- maximume0
- Direction
- maximume0
- Value
- 97%e0
- Qualifier
- one of the driest environmentse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“reaching a maximum of 97% in one of the driest environments”
e1“Transgenic wheat plants carrying a mutated version of HaHB4”
Claim · model-written summary
Transgenic plants showed positive trends in spikelets per spike, tillers per plant, and fertile florets per plant.
- Taxon
- wheatTriticum aestivume1
- Outcome
- spikelet numbers per spike, tillers per plant, and fertile florets per plante0
- Direction
- positive trendse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increased grain number per square meter of transgenic plants was accompanied by positive trends in spikelet numbers per spike, tillers per plant, and fertile florets per plant.”
e1“Transgenic wheat plants carrying a mutated version of HaHB4”
Claim · model-written summary
HaHB4 action was independent of responses triggered by RD19 and DREB1a.
- Taxon
- wheatTriticum aestivume1
- Gene
- HaHB4e0
- Outcome
- HaHB4's actione0
- Direction
- not dependente0
- Qualifier
- response triggered either by RD19 or by DREB1ae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HaHB4's action was not dependent on the response triggered either by RD19 or by DREB1a”
e1“Transgenic wheat plants carrying a mutated version of HaHB4”
The same questions in other papers
transgenic · physiology: water · Triticum aestivum (wheat)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
loss of function · physiology: water · Triticum aestivum (wheat)Positive and negative results
2 papers in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.