Abstract
BACKGROUND: Lipoxygenases (LOXs) play significant roles in abiotic stress responses, and identification of LOX gene promoter function can make an important contribution to elucidating resistance mechanisms. Here, we cloned the CmLOX08 promoter of melon (Cucumis melo) and identified the main promoter regions regulating transcription in response to signalling molecules and abiotic stresses. RESULTS: The 2054-bp promoter region of CmLOX08 from melon leaves was cloned, and bioinformatic analysis revealed that it harbours numerous cis-regulatory elements associated with signalling molecules and abiotic stress. Five 5'-deletion fragments obtained from the CmLOX08 promoter-2054 (LP1), 1639 (LP2), 1284 (LP3), 1047 (LP4), and 418 bp (LP5)-were fused with a GUS reporter gene and used for tobacco transient assays. Deletion analysis revealed that in response to abscisic acid, salicylic acid, and hydrogen peroxide, the GUS activity of LP1 was significantly higher than that of the mock-treated control and LP2, indicating that the - 2054- to - 1639-bp region positively regulates expression induced by these signalling molecules. However, no deletion fragment GUS activity was induced by methyl jasmonate. In response to salt, drought, and wounding treatments, LP1, LP2, and LP4 promoted significantly higher GUS expression compared with the control. Among all deletion fragments, LP4 showed the highest GUS expression, indicating that - 1047 to - 1 bp is the major region regulating promoter activity and that the - 1047 to - 418-bp region positively regulates expression induced by salt, drought, and wounding, whereas the - 1284 to - 1047-bp region is a negative regulatory segment. Interestingly, although the GUS activity of LP1 and LP2 was not affected by temperature changes, that of LP3 was significantly induced by heat, indicating that the - 1284- to - 1-bp region is a core sequence responding to heat and the - 2054- to - 1284-bp region negatively regulates expression induced by heat. Similarly, the - 1047- to - 1-bp region is the main sequence responding to cold, whereas the - 2054- to - 1047-bp region negatively regulates expression induced by cold. CONCLUSIONS: We cloned the CmLOX08 promoter and demonstrated that it is a signalling molecule/stress-inducible promoter. Furthermore, we identified core and positive/negative regulatory regions responding to three signalling molecules and five abiotic stresses.
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Claims 17
Claim · model-written summary
The LP1 CmLOX08 promoter fragment gave higher GUS activity than mock control and LP2 under abscisic acid
- Taxon
- melon
.jpg?width=60)
Cucumis meloe0 - Material
- LP1e1
- Treatment
- abscisic acide1
- Gene
- CmLOX08e0
- Outcome
- GUS activitye1
- Direction
- highere1
- Comparator
- mock-treated control and LP2e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“The 2054-bp promoter region of CmLOX08 from melon leaves was cloned”
e1“in response to abscisic acid, salicylic acid, and hydrogen peroxide, the GUS activity of LP1 was significantly higher than that of the mock-treated control and LP2”
Claim · model-written summary
The LP1 CmLOX08 promoter fragment gave higher GUS activity than mock control and LP2 under salicylic acid
- Taxon
- melonCucumis meloe0
- Material
- LP1e1
- Treatment
- salicylic acide1
- Gene
- CmLOX08e0
- Outcome
- GUS activitye1
- Direction
- highere1
- Comparator
- mock-treated control and LP2e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 2054-bp promoter region of CmLOX08 from melon leaves was cloned”
e1“in response to abscisic acid, salicylic acid, and hydrogen peroxide, the GUS activity of LP1 was significantly higher than that of the mock-treated control and LP2”
Claim · model-written summary
The LP1 CmLOX08 promoter fragment gave higher GUS activity than mock control and LP2 under hydrogen peroxide
- Taxon
- melonCucumis meloe0
- Material
- LP1e1
- Treatment
- hydrogen peroxidee1
- Gene
- CmLOX08e0
- Outcome
- GUS activitye1
- Direction
- highere1
- Comparator
- mock-treated control and LP2e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 2054-bp promoter region of CmLOX08 from melon leaves was cloned”
e1“in response to abscisic acid, salicylic acid, and hydrogen peroxide, the GUS activity of LP1 was significantly higher than that of the mock-treated control and LP2”
Claim · model-written summary
Methyl jasmonate did not induce GUS activity from any CmLOX08 promoter deletion fragment
- Taxon
- melonCucumis meloe0
- Treatment
- methyl jasmonatee2
- Outcome
- GUS activitye2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 2054-bp promoter region of CmLOX08 from melon leaves was cloned”
e2“However, no deletion fragment GUS activity was induced by methyl jasmonate”
Claim · model-written summary
The LP1, LP2 and LP4 fragments gave higher GUS expression than control under salt
- Taxon
- melonCucumis meloe0
- Material
- LP1, LP2, and LP4e3
- Treatment
- salte3
- Gene
- CmLOX08e0
- Outcome
- GUS expressione3
- Direction
- highere3
- Comparator
- controle3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 2054-bp promoter region of CmLOX08 from melon leaves was cloned”
e3“In response to salt, drought, and wounding treatments, LP1, LP2, and LP4 promoted significantly higher GUS expression compared with the control”
Claim · model-written summary
The LP1, LP2 and LP4 fragments gave higher GUS expression than control under drought
- Taxon
- melonCucumis meloe0
- Material
- LP1, LP2, and LP4e3
- Treatment
- droughte3
- Gene
- CmLOX08e0
- Outcome
- GUS expressione3
- Direction
- highere3
- Comparator
- controle3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 2054-bp promoter region of CmLOX08 from melon leaves was cloned”
e3“In response to salt, drought, and wounding treatments, LP1, LP2, and LP4 promoted significantly higher GUS expression compared with the control”
Claim · model-written summary
The LP1, LP2 and LP4 fragments gave higher GUS expression than control under wounding
- Taxon
- melonCucumis meloe0
- Material
- LP1, LP2, and LP4e3
- Treatment
- woundinge3
- Gene
- CmLOX08e0
- Outcome
- GUS expressione3
- Direction
- highere3
- Comparator
- controle3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 2054-bp promoter region of CmLOX08 from melon leaves was cloned”
e3“In response to salt, drought, and wounding treatments, LP1, LP2, and LP4 promoted significantly higher GUS expression compared with the control”
Claim · model-written summary
The LP4 fragment showed the highest GUS expression among all CmLOX08 deletion fragments
- Taxon
- melonCucumis meloe0
- Material
- LP4e4
- Outcome
- GUS expressione4
- Direction
- higheste4
- Comparator
- all deletion fragmentse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 2054-bp promoter region of CmLOX08 from melon leaves was cloned”
e4“Among all deletion fragments, LP4 showed the highest GUS expression”
Claim · model-written summary
The -1047 to -1 bp region is the major region regulating CmLOX08 promoter activity
- Taxon
- melonCucumis meloe0
- Material
- - 1047 to - 1 bpe5
- Outcome
- promoter activitye5
- Qualifier
- major regione5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 2054-bp promoter region of CmLOX08 from melon leaves was cloned”
e5“indicating that - 1047 to - 1 bp is the major region regulating promoter activity and that the - 1047 to - 418-bp region positively regulates expression induced by salt, drought, and wounding, whereas the - 1284 to - 1047-bp region is a negative regulatory segment”
Claim · model-written summary
The -1047 to -418 bp region positively regulates CmLOX08 expression induced by salt, drought and wounding
- Taxon
- melonCucumis meloe0
- Material
- - 1047 to - 418-bp regione5
- Outcome
- expression induced by salt, drought, and woundinge5
- Direction
- positively regulatese5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 2054-bp promoter region of CmLOX08 from melon leaves was cloned”
e5“indicating that - 1047 to - 1 bp is the major region regulating promoter activity and that the - 1047 to - 418-bp region positively regulates expression induced by salt, drought, and wounding, whereas the - 1284 to - 1047-bp region is a negative regulatory segment”
Claim · model-written summary
The -1284 to -1047 bp region is a negative regulatory segment of CmLOX08 promoter activity
- Taxon
- melonCucumis meloe0
- Material
- - 1284 to - 1047-bp regione5
- Outcome
- promoter activitye5
- Qualifier
- negative regulatory segmente5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 2054-bp promoter region of CmLOX08 from melon leaves was cloned”
e5“indicating that - 1047 to - 1 bp is the major region regulating promoter activity and that the - 1047 to - 418-bp region positively regulates expression induced by salt, drought, and wounding, whereas the - 1284 to - 1047-bp region is a negative regulatory segment”
Claim · model-written summary
LP3 expression was significantly induced by heat
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 2054-bp promoter region of CmLOX08 from melon leaves was cloned”
e6“Interestingly, although the GUS activity of LP1 and LP2 was not affected by temperature changes, that of LP3 was significantly induced by heat”
Claim · model-written summary
LP1 and LP2 GUS activity was not affected by temperature changes
- Taxon
- melonCucumis meloe0
- Material
- LP1 and LP2e6
- Treatment
- temperature changese6
- Outcome
- GUS activitye6
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 2054-bp promoter region of CmLOX08 from melon leaves was cloned”
e6“Interestingly, although the GUS activity of LP1 and LP2 was not affected by temperature changes, that of LP3 was significantly induced by heat”
Claim · model-written summary
The -1284 to -1 bp region is a core sequence responding to heat
- Taxon
- melonCucumis meloe0
- Material
- - 1284- to - 1-bp regione7
- Outcome
- core sequence responding to heate7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 2054-bp promoter region of CmLOX08 from melon leaves was cloned”
e7“indicating that the - 1284- to - 1-bp region is a core sequence responding to heat and the - 2054- to - 1284-bp region negatively regulates expression induced by heat”
Claim · model-written summary
The -2054 to -1284 bp region negatively regulates heat-induced CmLOX08 expression
- Taxon
- melonCucumis meloe0
- Material
- - 2054- to - 1284-bp regione7
- Outcome
- expression induced by heate7
- Qualifier
- negatively regulatese7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 2054-bp promoter region of CmLOX08 from melon leaves was cloned”
e7“indicating that the - 1284- to - 1-bp region is a core sequence responding to heat and the - 2054- to - 1284-bp region negatively regulates expression induced by heat”
Claim · model-written summary
The -1047 to -1 bp region is the main sequence responding to cold
- Taxon
- melonCucumis meloe0
- Material
- - 1047- to - 1-bp regione7
- Outcome
- main sequence responding to colde7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 2054-bp promoter region of CmLOX08 from melon leaves was cloned”
e7“Similarly, the - 1047- to - 1-bp region is the main sequence responding to cold, whereas the - 2054- to - 1047-bp region negatively regulates expression induced by cold”
Claim · model-written summary
The -2054 to -1047 bp region negatively regulates cold-induced CmLOX08 expression
- Taxon
- melonCucumis meloe0
- Material
- - 2054- to - 1047-bp regione7
- Outcome
- expression induced by colde7
- Qualifier
- negatively regulatese7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 2054-bp promoter region of CmLOX08 from melon leaves was cloned”
e7“Similarly, the - 1047- to - 1-bp region is the main sequence responding to cold, whereas the - 2054- to - 1047-bp region negatively regulates expression induced by cold”
The same questions in other papers
salicylic acid · molecular: expression · Cucumis melo (muskmelon)Results agree
2 papers in the same direction as this paper
- positive
- positive
salinity · molecular: expression · Cucumis melo (muskmelon)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.