Abstract
In nature, environmental factors highly influence the carotenoid composition in pumpkin plants and these factors were difficult to control; thus, carotenoid content is varied quantitatively and qualitatively. However, certain parameters can be controlled and this can be conducted in the laboratory through biogenesis manipulation. This approach uses environmental stress as a tool to alter the carotenoid pathway in the plants. The main objective of this study was to observe the inhibiting and enhancing effect of abiotic stress on individual carotenoid accumulation in pumpkin plants under light and dark conditions. The abiotic stresses used were plant elicitors which consisted of Ultra Violet light exposure, Polyethylene Glycol 4000, Salicylic Acid, and half strength nutrients using Murashige and Skoog Salt. After two weeks of treatments, the pumpkin leaves and stems were harvested, freeze dried and extracted to determine the carotenoids compound using High-Performance Liquid Chromatography (HPLC). Results showed that there was a significant difference (p<0.005) of lutein content (2.668 + 0.565 µg/g) in pumpkin leaves once exposed to Ultra Violet light with the absence of β-carotene compared to the control treatment; lutein (3.119 ± 0.210 µg/g) and β-carotene (0.838 + 0.05 µg/g). There were significant differences of carotenoids content under dark condition with the value of lutein at 0.472 + 0.008 µg/g to 1.247 + 0.047 µg/g and β-carotene from not detected to 1.360 + 0.003. The highest amount of lutein in pumpkin stem was detected in the Salicylic acid treatment under light condition (0.930 + 0.101 µg/g) and the highest amount of β-carotene in the pumpkin stem was detected in the Salicylic acid treatment under dark condition (0.234 + 0.018 µg/g). The carotenoid content varied in each treatment due to the adaptation of pumpkin plants with abiotic stress induced to them.
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Claims 6
Claim · model-written summary
UV exposed pumpkin leaves had 2.668 + 0.565 µg/g lutein, significantly different from control.
- Taxon
- pumpkine0
- Organ / stage
- leavese0
- Treatment
- Ultra Violet lighte0
- Outcome
- lutein contente0
- Value
- 2.668 + 0.565 µg/ge0
- Qualifier
- 3.119 ± 0.210 µg/ge0
- Comparator
- control treatmente0
Not reportedDirection
Verbatim
e0“significant difference (p<0.005) of lutein content (2.668 + 0.565 µg/g) in pumpkin leaves once exposed to Ultra Violet light with the absence of β-carotene compared to the control treatment; lutein (3.119 ± 0.210 µg/g)”
Claim · model-written summary
β-carotene was absent in UV exposed leaves versus 0.838 + 0.05 µg/g in controls.
- Taxon
- pumpkine0
- Organ / stage
- leavese0
- Treatment
- Ultra Violet lighte0
- Outcome
- absence of β-carotenee0
- Qualifier
- 0.838 + 0.05 µg/ge0
- Comparator
- control treatmente0
Not reportedDirection
Verbatim
e0“pumpkin leaves once exposed to Ultra Violet light with the absence of β-carotene compared to the control treatment; lutein (3.119 ± 0.210 µg/g) and β-carotene (0.838 + 0.05 µg/g)”
Claim · model-written summary
Under dark conditions, lutein content ranged from 0.472 + 0.008 to 1.247 + 0.047 µg/g.
Verbatim
e0“significant differences of carotenoids content under dark condition with the value of lutein at 0.472 + 0.008 µg/g to 1.247 + 0.047 µg/g”
t0“pumpkin plants”
Claim · model-written summary
Under dark conditions, β-carotene ranged from undetected to 1.360 + 0.003.
Verbatim
e0“β-carotene from not detected to 1.360 + 0.003”
t0“pumpkin plants”
Claim · model-written summary
Salicylic acid under light produced the highest stem lutein, 0.930 + 0.101 µg/g.
- Taxon
- pumpkine0
- Organ / stage
- steme0
- Treatment
- Salicylic acid treatmente0
- Outcome
- amount of luteine0
- Direction
- higheste0
- Value
- 0.930 + 0.101 µg/ge0
- Setting
- light conditione0
Not reportedComparator
Verbatim
e0“highest amount of lutein in pumpkin stem was detected in the Salicylic acid treatment under light condition (0.930 + 0.101 µg/g)”
Claim · model-written summary
Salicylic acid under dark produced the highest stem β-carotene, 0.234 + 0.018 µg/g.
- Taxon
- pumpkine0
- Organ / stage
- steme0
- Treatment
- Salicylic acid treatmente0
- Outcome
- amount of β-carotenee0
- Direction
- higheste0
- Value
- 0.234 + 0.018 µg/ge0
- Setting
- dark conditione0
Not reportedComparator
Verbatim
e0“highest amount of β-carotene in the pumpkin stem was detected in the Salicylic acid treatment under dark condition (0.234 + 0.018 µg/g)”
The same questions in other papers
salicylic acid · molecular: metabolites · Cucurbita (pumpkins)Results agree
2 papers in the same direction as this paper
- positive
- positive
light · molecular: metabolites · Cucurbita (pumpkins)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- positive
- no effect
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.