Paper · 2019 · International Journal of Current Microbiology and Applied Sciences

Isolation and Characterization of Indole Acetic Acid Producing Bacteria from Rhizosphere Soil and their Effect on Seed Germination

Sharnali Das; Tauhidur Rahman Nurunnabi; Rehana Parveen; et al.

4
claims
7
verbatim quotes
29
citations
1
research areas
✓
Open access · cc-by-nc-sa

Abstract

Indole acetic acid (IAA) is one of the most important phytohormones enhances the root structure and plant growth. The present study provides the isolation and characterization of IAA producing bacteria from rhizosphere soil and their effect on seed germination. Out of 30 isolates, eight (viz. Aux 4, Aux 9, Aux14, Aux16, Aux 19, Aux 20, Aux 21 and Aux 25) were able to produce IAA which was confirmed by Salkowski reagent test. Isolates were characterized on the basis of visual observations, gram staining and biochemical tests such as oxidase, catalase, indole, TSI, methyl red, Voges-Proskauer, citrate utilization, urease and starch hydrolysis test. The IAA producing bacteria are rod and round shaped gram positive and gram negative bacteria. In biochemical tests, both positive and negative responses were observed. Spectrometric analysis of IAA was done after 24, 48 and 72 hours of culture which showed that the isolated bacteria produced maximum concentration of IAA after 72 hours of incubation period at 37°C. The concentration was measured using a standard IAA curve and the maximum concentration was obtained by Aux 25. The in vitro treatment of maize and rice seeds with Aux 25 for germination showed better result than control. Further study is necessary for molecular characterization of isolated bacteria.

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Claims 4

Observationobservedoriginal resultmeasured

Claim · model-written summary

Eight of 30 rhizosphere isolates produced IAA.

Taxon
maize and riceOryza sativaZea mayse3
Interactor
isolatese0symbiont
Outcome
able to produce IAAe0
Value
eighte0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗
Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Out of 30 isolates, eight (viz. Aux 4, Aux 9, Aux14, Aux16, Aux 19, Aux 20, Aux 21 and Aux 25) were able to produce IAA”

✓ located verbatim in the source · abstract, characters 262–381 · show in text ↑
e3

“The in vitro treatment of maize and rice seeds with Aux 25 for germination showed better result than control”

✓ located verbatim in the source · abstract, characters 1134–1242 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Isolate Aux 25 gave the maximum IAA concentration.

Taxon
maize and riceOryza sativaZea mayse3
Interactor
Aux 25e1symbiont
Outcome
maximum concentratione1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗
Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“The concentration was measured using a standard IAA curve and the maximum concentration was obtained by Aux 25”

✓ located verbatim in the source · abstract, characters 1022–1132 · show in text ↑
e3

“The in vitro treatment of maize and rice seeds with Aux 25 for germination showed better result than control”

✓ located verbatim in the source · abstract, characters 1134–1242 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The isolated bacteria produced maximum IAA concentration after 72 hours of incubation at 37°C.

Taxon
maize and riceOryza sativaZea mayse3
Interactor
isolated bacteriae2symbiont
Outcome
produced maximum concentration of IAAe2
Qualifier
after 72 hours of incubation period at 37°Ce2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗
Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e2

“the isolated bacteria produced maximum concentration of IAA after 72 hours of incubation period at 37°C”

✓ located verbatim in the source · abstract, characters 917–1020 · show in text ↑
e3

“The in vitro treatment of maize and rice seeds with Aux 25 for germination showed better result than control”

✓ located verbatim in the source · abstract, characters 1134–1242 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Seed treatment with Aux 25 gave better germination of maize and rice than the control.

Taxon
maize and riceOryza sativaZea mayse3
Treatment
in vitro treatment of maize and rice seeds with Aux 25e3
Interactor
Aux 25e3symbiont
Outcome
germinatione3
Direction
bettere3
Comparator
controle3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗
Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e3

“The in vitro treatment of maize and rice seeds with Aux 25 for germination showed better result than control”

✓ located verbatim in the source · abstract, characters 1134–1242 · show in text ↑

Oryza sativa · growth · Zea mays (maize)Positive and negative results

35 papers: 31 positive, 4 negative or no effect

30 papers in the same direction as this paper · 4 in the opposite direction

  1. negative
  2. negative
  3. negative

31 papers more in this group

See all 35 papers →

Zea mays · growth · Oryza sativa (rice)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

in vitro · growth · Zea mays (maize)Positive and negative results

87 papers: 60 positive, 27 negative or no effect

59 papers in the same direction as this paper · 26 in the opposite direction · 1 reporting no difference from an alternative

  1. negative
  2. negative
  3. negative

83 papers more in this group

See all 87 papers →

in vitro · growth · Oryza sativa (rice)Positive and negative results

165 papers: 140 positive, 25 negative or no effect

139 papers in the same direction as this paper · 24 in the opposite direction · 1 reporting no difference from an alternative

  1. negative
  2. negative
  3. negative

161 papers more in this group

See all 165 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.