Abstract
Indole acetic acid (IAA) is one of the most important phytohormones enhances the root structure and plant growth. The present study provides the isolation and characterization of IAA producing bacteria from rhizosphere soil and their effect on seed germination. Out of 30 isolates, eight (viz. Aux 4, Aux 9, Aux14, Aux16, Aux 19, Aux 20, Aux 21 and Aux 25) were able to produce IAA which was confirmed by Salkowski reagent test. Isolates were characterized on the basis of visual observations, gram staining and biochemical tests such as oxidase, catalase, indole, TSI, methyl red, Voges-Proskauer, citrate utilization, urease and starch hydrolysis test. The IAA producing bacteria are rod and round shaped gram positive and gram negative bacteria. In biochemical tests, both positive and negative responses were observed. Spectrometric analysis of IAA was done after 24, 48 and 72 hours of culture which showed that the isolated bacteria produced maximum concentration of IAA after 72 hours of incubation period at 37°C. The concentration was measured using a standard IAA curve and the maximum concentration was obtained by Aux 25. The in vitro treatment of maize and rice seeds with Aux 25 for germination showed better result than control. Further study is necessary for molecular characterization of isolated bacteria.
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Claims 4
Claim · model-written summary
Eight of 30 rhizosphere isolates produced IAA.
- Taxon
- maize and rice
Oryza sativa
Zea mayse3 - Interactor
- isolatese0symbiont
- Outcome
- able to produce IAAe0
- Value
- eighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Out of 30 isolates, eight (viz. Aux 4, Aux 9, Aux14, Aux16, Aux 19, Aux 20, Aux 21 and Aux 25) were able to produce IAA”
e3“The in vitro treatment of maize and rice seeds with Aux 25 for germination showed better result than control”
Claim · model-written summary
Isolate Aux 25 gave the maximum IAA concentration.
- Taxon
- maize and rice
Oryza sativa
Zea mayse3 - Interactor
- Aux 25e1symbiont
- Outcome
- maximum concentratione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e1“The concentration was measured using a standard IAA curve and the maximum concentration was obtained by Aux 25”
e3“The in vitro treatment of maize and rice seeds with Aux 25 for germination showed better result than control”
Claim · model-written summary
The isolated bacteria produced maximum IAA concentration after 72 hours of incubation at 37°C.
- Taxon
- maize and rice
Oryza sativa
Zea mayse3 - Interactor
- isolated bacteriae2symbiont
- Outcome
- produced maximum concentration of IAAe2
- Qualifier
- after 72 hours of incubation period at 37°Ce2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e2“the isolated bacteria produced maximum concentration of IAA after 72 hours of incubation period at 37°C”
e3“The in vitro treatment of maize and rice seeds with Aux 25 for germination showed better result than control”
Claim · model-written summary
Seed treatment with Aux 25 gave better germination of maize and rice than the control.
- Taxon
- maize and rice
Oryza sativa
Zea mayse3 - Treatment
- in vitro treatment of maize and rice seeds with Aux 25e3
- Interactor
- Aux 25e3symbiont
- Outcome
- germinatione3
- Direction
- bettere3
- Comparator
- controle3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e3“The in vitro treatment of maize and rice seeds with Aux 25 for germination showed better result than control”
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