Abstract
The optimum condition for shoot regeneration from leaf explants of strawberry cultivar Tiogar was investigated. It was found that the best regeneration condition was MS medium containing N6-Benzyladenine (BA) and 2,4-Dichlorophenoxy acetic acid (2,4-D) at concentrations of 1 mg.l-1 and 0.2 mg.l-1, respectively. Antibiotics sensitivity test found that shoot regeneration from leaf explant was inhibited more than 90% at the concentration of kanamycin (Km) as low as 5 mg.l-1. The modified gene encoding antifreeze protein isoform HPLC 6 was successfully constructed using codons which were optimally expressed in the strawberry plant. The antifreeze protein genes, naturally in plasmid pSW1 and modified in plasmid BB, were transformed to strawberry leaf explants by Agrobacterium tumefaciens LBA 4404. The strawberry plants, transformed with both AFP genes, were able to root in MS media containing 50 mg.l-1 Km, while no roots grew from nontransformed plant in this condition. Polymerase chain reaction indicated that the transgenes were integrated in the genome of transformants.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-sa. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
MS medium with 1 mg/l BA and 0.2 mg/l 2,4-D was the best condition for shoot regeneration from strawberry leaf explants.
- Taxon
- strawberry
_single2.jpg?width=60)
Fragaria ×ananassae1 - Material
- Tiogare1
- Organ / stage
- leaf explantse1
- Treatment
- MS medium containing N6-Benzyladenine (BA) and 2,4-Dichlorophenoxy acetic acid (2,4-D) at concentrations of 1 mg.l-1 and 0.2 mg.l-1e2
- Outcome
- shoot regeneratione1
- Direction
- beste2
- Value
- 1 mg.l-1 and 0.2 mg.l-1e2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e1“shoot regeneration from leaf explants of strawberry cultivar Tiogar”
e2“the best regeneration condition was MS medium containing N6-Benzyladenine (BA) and 2,4-Dichlorophenoxy acetic acid (2,4-D) at concentrations of 1 mg.l-1 and 0.2 mg.l-1”
Claim · model-written summary
Kanamycin at 5 mg/l inhibited shoot regeneration from strawberry leaf explants by more than 90%.
- Taxon
- strawberryFragaria ×ananassae1
- Treatment
- kanamycin (Km)e3
- Outcome
- shoot regeneration from leaf explante3
- Direction
- inhibitede3
- Value
- more than 90%e3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“shoot regeneration from leaf explants of strawberry cultivar Tiogar”
e3“shoot regeneration from leaf explant was inhibited more than 90% at the concentration of kanamycin (Km) as low as 5 mg.l-1”
Claim · model-written summary
Transformed strawberry plants rooted on 50 mg/l Km, whereas nontransformed plants formed no roots.
- Taxon
- strawberryFragaria ×ananassae1
- Treatment
- 50 mg.l-1 Kme4
- Gene
- AFP genese4
- Outcome
- rootse4
- Direction
- able to roote4
- Comparator
- nontransformed plante4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“shoot regeneration from leaf explants of strawberry cultivar Tiogar”
e4“The strawberry plants, transformed with both AFP genes, were able to root in MS media containing 50 mg.l-1 Km, while no roots grew from nontransformed plant in this condition.”
Claim · model-written summary
PCR indicated that the transgenes were integrated into the genome of the strawberry transformants.
- Taxon
- strawberryFragaria ×ananassae1
- Outcome
- the transgenes were integrated in the genomee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“shoot regeneration from leaf explants of strawberry cultivar Tiogar”
e5“Polymerase chain reaction indicated that the transgenes were integrated in the genome of transformants.”
Claim · model-written summary
The modified antifreeze protein gene (isoform HPLC 6) with strawberry-optimized codons was successfully constructed.
- Taxon
- strawberryFragaria ×ananassae1
- Gene
- antifreeze protein isoform HPLC 6e6
- Outcome
- modified gene encoding antifreeze protein isoform HPLC 6e6
- Direction
- successfully constructede6
- Qualifier
- codons which were optimally expressed in the strawberry plante6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“shoot regeneration from leaf explants of strawberry cultivar Tiogar”
e6“The modified gene encoding antifreeze protein isoform HPLC 6 was successfully constructed using codons which were optimally expressed in the strawberry plant.”
The same questions in other papers
N-benzyladenine · growth · Fragaria x ananassa (strawberry)Results differ
51 papers in the same direction as this paper · 4 in the opposite direction
- negative
- no effect
- negative
52 papers more in this group
See all 56 papers →2,4-D · growth · Fragaria x ananassa (strawberry)Positive and negative results
6 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
4 papers more in this group
See all 8 papers →acetic acid · growth · Fragaria x ananassa (strawberry)Results agree
1 paper in the same direction as this paper
- positive
in vitro · growth · Fragaria x ananassa (strawberry)Positive and negative results
75 papers in the same direction as this paper · 9 in the opposite direction · 1 reporting no difference from an alternative
- positive
- negative
- no effect
82 papers more in this group
See all 86 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.