Abstract
Somatic embryo-like structures (SELS) were produced in vitro from leaf disk and petiole explants of two cultivars of strawberry (Fragaria x ananassa Duch) on Murashige and Skoog medium with different concentrations and combinations of 2,4-dichlorophenoxyacetic acid (2,4-D), 6-benzylaminopurine (BAP) and sucrose to check the embryonic nature of these structures histologically. A large number of SELS could be regenerated in both cultivars on media with 2-4 mg L(-1) 2,4-D in combination with 0.5 -1 mg L(-1) BAP and 50 g x L(-1) sucrose. Histological examination of SELS revealed the absence of a root pole. Therefore these structures cannot be strictly classified as somatic embryos. The SELS formed under the tested culture conditions represent malformed shoot-like and leaf-like structures. The importance of these results for the propagation of strawberries via somatic embryogenesis is discussed.
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Claims 3
Claim · model-written summary
Both strawberry cultivars regenerated many somatic embryo-like structures on the specified medium.
- Taxon
- strawberry
_single2.jpg?width=60)
Fragaria ×ananassat0 - Treatment
- 2-4 mg L(-1) 2,4-D in combination with 0.5 -1 mg L(-1) BAP and 50 g x L(-1) sucrosee0
- Outcome
- SELSe0
- Direction
- A large numbere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“A large number of SELS could be regenerated in both cultivars on media with 2-4 mg L(-1) 2,4-D in combination with 0.5 -1 mg L(-1) BAP and 50 g x L(-1) sucrose”
t0“strawberry”
Claim · model-written summary
Histological examination found no root pole in strawberry somatic embryo-like structures.
- Taxon
- strawberryFragaria ×ananassat0
- Outcome
- absence of a root polee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Histological examination of SELS revealed the absence of a root pole”
t0“strawberry”
Claim · model-written summary
The strawberry structures were judged malformed shoot-like and leaf-like structures rather than true somatic embryos.
- Taxon
- strawberryFragaria ×ananassat0
- Outcome
- malformed shoot-like and leaf-like structurese0
- Qualifier
- cannot be strictly classified as somatic embryose0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Therefore these structures cannot be strictly classified as somatic embryos. The SELS formed under the tested culture conditions represent malformed shoot-like and leaf-like structures.”
t0“strawberry”
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