Abstract
Prospecting of local grapevine (Vitis vinifera L.) germplasm revealed that Tunisia possesses a rich patrimony which presents diversified organoleptic characteristics. However, viral diseases seriously affect all local grapevine cultivars which risk a complete extinction. Sanitation programs need to be established to preserve and exploit, as a gene pool, the Tunisian vineyards areas. The presence of the Grapevine leafroll associated virus-3 (GLRaV-3), Grapevine stem pitting associated virus (GRSPaV) and Grapevine virus A (GVA), were confirmed in a Tunisian grapevine cultivar using serological and molecular analyses. The association between GRSPaV and GVA viruses induces more rugose wood symptoms and damages. For this reason the cleansing of the infected cultivar is highly advisable. Direct and recurrent somatic embryos of cv. 'Hencha' were successfully induced from filament, when cultured on Che and Pool (1987). based-medium, enriched with 2 mg 1 -1 of 2,4-dichlorophenoxyacetic acid and 2.5 mg 1 -1 of Thidiazuron, after 36 weeks of culture. After six months of acclimatization, RT-PCR carried on 50 somaplants confirmed the absence of GVA, GRSPa-V as well as GLRaV-3 viruses in all somaplants. Ampelographic analysis, based on eight OIV descriptors, was carried out on two years acclimated somaplants, compared to the mother plant. Results demonstrated that the shape and contours of 46 somaclones leaves are identical to mother plant leaves and four phenotypically off-type plants were observed. The healthy state of 100% 'Hencha' somaclones and the high percentage of phenotypically true-to-type plants demonstrate that somatic embryogenesis is a promising technique to adopt for grapevine viruses elimination.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
Three grapevine viruses were confirmed in the Tunisian cultivar.
- Interactor
- Grapevine leafroll associated virus-3 (GLRaV-3)e0pathogen
- Second interactor
- Grapevine stem pitting associated virus (GRSPaV)Grapevine rupestris stem pitting-associated viruse0pathogen
- Outcome
- presence of the Grapevine leafroll associated virus-3 (GLRaV-3), Grapevine stem pitting associated virus (GRSPaV) and Grapevine virus A (GVA)e0
VitisOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The presence of the Grapevine leafroll associated virus-3 (GLRaV-3), Grapevine stem pitting associated virus (GRSPaV) and Grapevine virus A (GVA), were confirmed in a Tunisian grapevine cultivar”
Claim · model-written summary
GRSPaV and GVA together induced more rugose wood symptoms and damage.
- Interactor
- GRSPaVGrapevine rupestris stem pitting-associated viruse0pathogen
- Second interactor
- GVAe0pathogen
- Outcome
- rugose wood symptoms and damagese0
- Direction
- moree0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The association between GRSPaV and GVA viruses induces more rugose wood symptoms and damages.”
t0“Elimination of Grapevine”
Claim · model-written summary
Direct and recurrent embryos were successfully induced from Hencha filaments after 36 weeks on the enriched medium.
- Material
- cv. 'Hencha'e0
- Treatment
- Che and Pool (1987). based-medium, enriched with 2 mg 1 -1 of 2,4-dichlorophenoxyacetic acid and 2.5 mg 1 -1 of Thidiazurone0
- Outcome
- somatic embryose0
- Qualifier
- after 36 weeks of culturee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Direct and recurrent somatic embryos of cv. 'Hencha' were successfully induced from filament, when cultured on Che and Pool (1987). based-medium, enriched with 2 mg 1 -1 of 2,4-dichlorophenoxyacetic acid and 2.5 mg 1 -1 of Thidiazuron, after 36 weeks of culture.”
t0“Elimination of Grapevine”
Claim · model-written summary
All 50 acclimatized somaplants lacked the three tested viruses.
- Material
- Tunisian Cultivart0
- Interactor
- GVAe0pathogen
- Second interactor
- GRSPa-Ve0pathogen
- Outcome
- absence of GVA, GRSPa-V as well as GLRaV-3 virusese0
- Value
- 50 somaplantse0
- Qualifier
- After six months of acclimatizatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After six months of acclimatization, RT-PCR carried on 50 somaplants confirmed the absence of GVA, GRSPa-V as well as GLRaV-3 viruses in all somaplants.”
t0“Grapevine virus A from a Tunisian Cultivar”
Claim · model-written summary
Forty-six somaclones had leaf shapes and contours identical to the mother plant.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the shape and contours of 46 somaclones leaves are identical to mother plant leaves”
t0“Elimination of Grapevine”
Claim · model-written summary
Four somaclones were phenotypically off type.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“four phenotypically off-type plants were observed”
t0“Elimination of Grapevine”
Claim · model-written summary
The authors considered somatic embryogenesis promising for grapevine virus elimination.
- Outcome
- somatic embryogenesis is a promising technique to adopt for grapevine viruses eliminatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“somatic embryogenesis is a promising technique to adopt for grapevine viruses elimination.”
The same questions in other papers
2,4-D · growth · VitisPositive and negative results
3 papers in the same direction as this paper · 5 in the opposite direction
- negative
- negative
- negative
5 papers more in this group
See all 9 papers →Thidiazuron · growth · VitisResults agree
5 papers in the same direction as this paper
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.