Abstract
This study aimed to investigate the attitude of three nematode and phylloxera resistant grape rootstocks (Freedom, Ramsey and SO4) through the micropropagation stages till be micrografted by Superior scions. During that the effect of paclobutrazol (PP333) on rootstocks stem diameter, transverse sections in graft union regions and acclimatization of resulted micrografts were studied. The results proved that the most effective sterilization durations using 1% NaOCl were 15 min for Freedom and SO4 explants as they achieved 0.00 and 0.00%of contamination and 100% and 90% of survival percentages respectively, while 10 min duration was sufficient for Ramsey explants as it achieved 0.00 % contamination and 100% survival percentages. Significantly, Ramsey achieved the highest number of shoots (1.25), Freedom gave the tallest shoots (4.13 cm)and SO4 got the highest leaf number (7.33) all on MS medium. In multiplication stage; Freedom rootstock proved to be the most reacted rootstock as it significantly accomplished the highest shoot number (2.00) when planted on BA medium, the tallest shoots (3.54 cm) when planted on TDZ medium and the highest leaf number on Kin medium. Whereas, in order to get thicker shoots for easier grafting; using of PP333 at 5 mgl-1 significantly was the best for Freedom (3.55 mm) followed by SO4 (3.03 mm) and Ramsey (2.85 mm) rootstocks. In micrografting stage, Superior micrografts significantly achieved the best results on rooted Freedom rootstocksin scion survival (100.00%) and on un rooted rootstocks in scion bud burst (75.00 %), graft union formation (50.00%), rooting (75.00%). On the same trend, Freedom rootstock was more active in cell dividing activity when grafted by Superior cultivar compared to Ramsey and SO4 rootstocks which were very poor in producing callus cells in graft union. Finally, Freedom rootstock grafted with Superior gained the highest survival (100%) after acclimatization.
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Claims 20
Claim · model-written summary
Freedom explants sterilized with 1% NaOCl for 15 minutes had zero contamination.
- Taxon
- GRAPEt0
- Material
- Freedome0
- Treatment
- 1% NaOCle0
- Outcome
- contaminatione0
- Direction
- most effectivee0
- Value
- 0.00e0
- Qualifier
- 15 mine0
Not reportedComparator
Verbatim
e0“the most effective sterilization durations using 1% NaOCl were 15 min for Freedom and SO4 explants as they achieved 0.00 and 0.00%of contamination and 100% and 90% of survival percentages respectively”
t0“GRAPE ROOTSTOCKS”
Claim · model-written summary
SO4 explants sterilized with 1% NaOCl for 15 minutes had zero contamination.
- Taxon
- GRAPEt0
- Material
- SO4e0
- Treatment
- 1% NaOCle0
- Outcome
- contaminatione0
- Direction
- most effectivee0
- Value
- 0.00%e0
- Qualifier
- 15 mine0
Not reportedComparator
Verbatim
e0“the most effective sterilization durations using 1% NaOCl were 15 min for Freedom and SO4 explants as they achieved 0.00 and 0.00%of contamination and 100% and 90% of survival percentages respectively”
t0“GRAPE ROOTSTOCKS”
Claim · model-written summary
Freedom survival after 15-minute sterilization was 100%.
Not reportedDirection
Verbatim
e0“15 min for Freedom and SO4 explants as they achieved 0.00 and 0.00%of contamination and 100% and 90% of survival percentages respectively”
t0“GRAPE ROOTSTOCKS”
Claim · model-written summary
SO4 survival after 15-minute sterilization was 90%.
Not reportedDirection
Verbatim
e0“15 min for Freedom and SO4 explants as they achieved 0.00 and 0.00%of contamination and 100% and 90% of survival percentages respectively”
t0“GRAPE ROOTSTOCKS”
Claim · model-written summary
Ten-minute sterilization was sufficient for Ramsey explants, with zero contamination.
Not reportedDirection
Verbatim
e0“10 min duration was sufficient for Ramsey explants as it achieved 0.00 % contamination and 100% survival percentages”
t0“GRAPE ROOTSTOCKS”
Claim · model-written summary
Ramsey explants had 100% survival after ten-minute sterilization.
Not reportedDirection
Verbatim
e0“10 min duration was sufficient for Ramsey explants as it achieved 0.00 % contamination and 100% survival percentages”
t0“GRAPE ROOTSTOCKS”
Claim · model-written summary
Ramsey had the highest shoot number on MS medium, 1.25.
- Taxon
- GRAPEt0
- Material
- Ramseye0
- Treatment
- MS mediume0
- Outcome
- number of shootse0
- Direction
- higheste0
- Value
- 1.25e0
Not reportedComparator
Verbatim
e0“Significantly, Ramsey achieved the highest number of shoots (1.25), Freedom gave the tallest shoots (4.13 cm)and SO4 got the highest leaf number (7.33) all on MS medium.”
t0“GRAPE ROOTSTOCKS”
Claim · model-written summary
Freedom had the tallest shoots on MS medium, 4.13 cm.
Not reportedComparator
Verbatim
e0“Significantly, Ramsey achieved the highest number of shoots (1.25), Freedom gave the tallest shoots (4.13 cm)and SO4 got the highest leaf number (7.33) all on MS medium.”
t0“GRAPE ROOTSTOCKS”
Claim · model-written summary
SO4 had the highest leaf number on MS medium, 7.33.
Not reportedComparator
Verbatim
e0“Significantly, Ramsey achieved the highest number of shoots (1.25), Freedom gave the tallest shoots (4.13 cm)and SO4 got the highest leaf number (7.33) all on MS medium.”
t0“GRAPE ROOTSTOCKS”
Claim · model-written summary
Freedom multiplication on BA medium produced the highest shoot number, 2.00.
Not reportedComparator
Verbatim
e0“Freedom rootstock proved to be the most reacted rootstock as it significantly accomplished the highest shoot number (2.00) when planted on BA medium, the tallest shoots (3.54 cm) when planted on TDZ medium and the highest leaf number on Kin medium.”
t0“GRAPE ROOTSTOCKS”
Claim · model-written summary
Freedom multiplication on TDZ medium produced the tallest shoots, 3.54 cm.
Not reportedComparator
Verbatim
e0“Freedom rootstock proved to be the most reacted rootstock as it significantly accomplished the highest shoot number (2.00) when planted on BA medium, the tallest shoots (3.54 cm) when planted on TDZ medium and the highest leaf number on Kin medium.”
t0“GRAPE ROOTSTOCKS”
Claim · model-written summary
Freedom multiplication on Kin medium produced the highest leaf number.
Not reportedComparator
Verbatim
e0“Freedom rootstock proved to be the most reacted rootstock as it significantly accomplished the highest shoot number (2.00) when planted on BA medium, the tallest shoots (3.54 cm) when planted on TDZ medium and the highest leaf number on Kin medium.”
t0“GRAPE ROOTSTOCKS”
Claim · model-written summary
PP333 at 5 mgl-1 gave the best shoot thickness for Freedom, 3.55 mm.
- Taxon
- GRAPEt0
- Material
- Freedome0
- Treatment
- PP333 at 5 mgl-1e0
- Outcome
- thicker shootse0
- Direction
- beste0
- Value
- 3.55 mme0
Not reportedComparator
Verbatim
e0“in order to get thicker shoots for easier grafting; using of PP333 at 5 mgl-1 significantly was the best for Freedom (3.55 mm) followed by SO4 (3.03 mm) and Ramsey (2.85 mm) rootstocks.”
t0“GRAPE ROOTSTOCKS”
Claim · model-written summary
SO4 shoot thickness with PP333 at 5 mgl-1 was 3.03 mm, below Freedom.
Verbatim
e0“in order to get thicker shoots for easier grafting; using of PP333 at 5 mgl-1 significantly was the best for Freedom (3.55 mm) followed by SO4 (3.03 mm) and Ramsey (2.85 mm) rootstocks.”
t0“GRAPE ROOTSTOCKS”
Claim · model-written summary
Ramsey shoot thickness with PP333 at 5 mgl-1 was 2.85 mm, below Freedom.
Verbatim
e0“in order to get thicker shoots for easier grafting; using of PP333 at 5 mgl-1 significantly was the best for Freedom (3.55 mm) followed by SO4 (3.03 mm) and Ramsey (2.85 mm) rootstocks.”
t0“GRAPE ROOTSTOCKS”
Claim · model-written summary
Superior scion survival was best on rooted Freedom, at 100.00%.
- Taxon
- GRAPEt0
- Material
- Superiore0
- Treatment
- rooted Freedome0
- Outcome
- scion survivale0
- Direction
- beste0
- Value
- 100.00%e0
Not reportedComparator
Verbatim
e0“Superior micrografts significantly achieved the best results on rooted Freedom rootstocksin scion survival (100.00%) and on un rooted rootstocks in scion bud burst (75.00 %), graft union formation (50.00%), rooting (75.00%).”
t0“GRAPE ROOTSTOCKS”
Claim · model-written summary
Superior scion bud burst on unrooted rootstocks was best at 75.00%.
- Taxon
- GRAPEt0
- Material
- Superiore0
- Treatment
- un rooted rootstockse0
- Outcome
- scion bud burste0
- Direction
- beste0
- Value
- 75.00 %e0
Not reportedComparator
Verbatim
e0“Superior micrografts significantly achieved the best results on rooted Freedom rootstocksin scion survival (100.00%) and on un rooted rootstocks in scion bud burst (75.00 %), graft union formation (50.00%), rooting (75.00%).”
t0“GRAPE ROOTSTOCKS”
Claim · model-written summary
Superior graft union formation on unrooted rootstocks was best at 50.00%.
- Taxon
- GRAPEt0
- Material
- Superiore0
- Treatment
- un rooted rootstockse0
- Outcome
- graft union formatione0
- Direction
- beste0
- Value
- 50.00%e0
Not reportedComparator
Verbatim
e0“Superior micrografts significantly achieved the best results on rooted Freedom rootstocksin scion survival (100.00%) and on un rooted rootstocks in scion bud burst (75.00 %), graft union formation (50.00%), rooting (75.00%).”
t0“GRAPE ROOTSTOCKS”
Claim · model-written summary
Superior rooting on unrooted rootstocks was best at 75.00%.
- Taxon
- GRAPEt0
- Material
- Superiore0
- Treatment
- un rooted rootstockse0
- Outcome
- rootinge0
- Direction
- beste0
- Value
- 75.00%e0
Not reportedComparator
Verbatim
e0“Superior micrografts significantly achieved the best results on rooted Freedom rootstocksin scion survival (100.00%) and on un rooted rootstocks in scion bud burst (75.00 %), graft union formation (50.00%), rooting (75.00%).”
t0“GRAPE ROOTSTOCKS”
Claim · model-written summary
Freedom grafted with Superior had more cell division than Ramsey and SO4 and the highest survival after acclimatization, 100%.
Verbatim
e0“Freedom rootstock was more active in cell dividing activity when grafted by Superior cultivar compared to Ramsey and SO4 rootstocks which were very poor in producing callus cells in graft union.”
e1“Freedom rootstock grafted with Superior gained the highest survival (100%) after acclimatization.”
t0“GRAPE ROOTSTOCKS”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.