Abstract
Sorghum, a naturally drought tolerant crop, is genetically diverse and provides a wide gene pool for exploitation in crop breeding. In this study, we experimentally assessed friable callus induction rates of seven sorghum varieties using shoot explant for the generation of cell suspension cultures. The cell suspensions were characterized in terms of cell growth and viability profiles as well as gene expression following 400 mM sorbitol-induced osmotic stress for 72 h. Only ICSB 338, a drought susceptible variety, was readily amenable to friable callus formation. Cell culture growth plots of both ICSB 338 and White sorghum (used as a reference line) depicted typical sigmoidal curves. Interestingly, Evans blue assay showed that ICSB 338 cell cultures are more susceptible to osmotic stress than the White sorghum cells. The osmotic stress treatment also triggered differential expression of eight target genes between the two cell culture lines. Overall, these results suggest that the genetic diversity of sorghum germplasm influences friable callus induction rates and molecular responses to osmotic stress, and could be further exploited in plant stress biology studies. Therefore, we have developed a valuable resource for use in molecular studies of sorghum in response to a range of biotic and abiotic stresses.
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Claims 6
Claim · model-written summary
Only ICSB 338 readily formed friable callus.
- Taxon
- Sorghum bicolor (L.) Moench
Sorghum bicolort0 - Material
- ICSB 338e0
- Outcome
- friable callus formatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sorghum bicolor (L.) Moench”
e0“Only ICSB 338, a drought susceptible variety, was readily amenable to friable callus formation.”
Claim · model-written summary
ICSB 338 cell cultures showed sigmoidal growth.
- Taxon
- Sorghum bicolor (L.) MoenchSorghum bicolort0
- Material
- ICSB 338e0
- Outcome
- typical sigmoidal curvese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sorghum bicolor (L.) Moench”
e0“Cell culture growth plots of both ICSB 338 and White sorghum (used as a reference line) depicted typical sigmoidal curves.”
Claim · model-written summary
White sorghum cell cultures showed sigmoidal growth.
- Taxon
- Sorghum bicolor (L.) MoenchSorghum bicolort0
- Material
- White sorghume0
- Outcome
- typical sigmoidal curvese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sorghum bicolor (L.) Moench”
e0“Cell culture growth plots of both ICSB 338 and White sorghum (used as a reference line) depicted typical sigmoidal curves.”
Claim · model-written summary
ICSB 338 cultures were more susceptible to osmotic stress than White sorghum cells.
- Taxon
- Sorghum bicolor (L.) MoenchSorghum bicolort0
- Material
- ICSB 338e0
- Treatment
- osmotic stresse0
- Outcome
- susceptible to osmotic stresse0
- Direction
- more susceptiblee0
- Comparator
- White sorghum cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sorghum bicolor (L.) Moench”
e0“Evans blue assay showed that ICSB 338 cell cultures are more susceptible to osmotic stress than the White sorghum cells.”
Claim · model-written summary
Osmotic stress elicited differential expression of eight target genes between the cell lines.
- Taxon
- Sorghum bicolor (L.) MoenchSorghum bicolort0
- Treatment
- osmotic stress treatmente0
- Outcome
- differential expression of eight target genese0
- Value
- eight target genese0
- Comparator
- two cell culture linese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sorghum bicolor (L.) Moench”
e0“The osmotic stress treatment also triggered differential expression of eight target genes between the two cell culture lines.”
Claim · model-written summary
The authors developed sorghum cell cultures as a resource for molecular stress studies.
- Taxon
- Sorghum bicolor (L.) MoenchSorghum bicolort0
- Outcome
- valuable resource for use in molecular studies of sorghume0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sorghum bicolor (L.) Moench”
e0“we have developed a valuable resource for use in molecular studies of sorghum in response to a range of biotic and abiotic stresses.”
The same questions in other papers
drought · disease · Sorghum bicolor (sorghum)Positive and negative results
3 in the opposite direction
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.