Abstract
Histone H2B protein is not only structurally important for chromosomal DNA packaging but is also involved in regulation of gene expression, including the immune response of plants against pathogens. In this study, we show that potato virus X (PVX) infection resulted in reduced expression of H2B at both the mRNA and protein level in Nicotiana benthamiana. Tobacco rattle virus (TRV)-based virus-induced gene silencing (VIGS) was then used to down-regulate the expression of H2B in N. benthamiana and tests showed that the titre of TRV was similar in these plants to that in control treated plants. When these H2B-silenced plants were inoculated with PVX, the virus spread more slowly through the plant and there was a lower titre of PVX compared to non-silenced plants. Abnormal leaf development and stem necrosis were observed in the H2B-silenced plants, which were alleviated in H2B-silenced NahG transgenic plants suggesting the involvement of salicylic acid (SA) in the production of these symptoms. Indeed, quantitative reverse transcription (qRT)-PCR and liquid chromatography tandem mass spectroscopy (LC-MS) results showed that endogenous SA is increased in H2B-silenced N. benthamiana. Thus, downregulation of H2B induced accumulation of endogenous SA, which was correlated with stem necrosis and a decreased accumulation of PVX in N.benthamiana.
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Claims 8
Claim · model-written summary
PVX infection reduced H2B mRNA and protein expression in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- potato virus X (PVX) infectione0
- Interactor
- potato virus X (PVX)Potato virus Xe0pathogen
- Gene
- H2Be0
- Outcome
- expression of H2B at both the mRNA and protein levele0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“potato virus X (PVX) infection resulted in reduced expression of H2B at both the mRNA and protein level in Nicotiana benthamiana”
Claim · model-written summary
TRV titre was similar in H2B-silenced and control plants.
- Taxon
- N. benthamianaNicotiana benthamianae1
- Treatment
- H2Be1
- Interactor
- TRVTobacco rattle viruse0pathogen
- Gene
- H2Be1
- Outcome
- titre of TRVe0
- Direction
- similare0
- Comparator
- control treated plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“titre of TRV was similar in these plants to that in control treated plants”
e1“H2B in N. benthamiana”
Claim · model-written summary
PVX spread more slowly through H2B-silenced plants than non-silenced plants.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- H2B-silencede0
- Interactor
- PVXPotato virus Xe0pathogen
- Gene
- H2Be0
- Outcome
- virus spreade0
- Direction
- more slowlye0
- Comparator
- non-silenced plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When these H2B-silenced plants were inoculated with PVX, the virus spread more slowly through the plant and there was a lower titre of PVX compared to non-silenced plants”
t0“Nicotiana benthamiana”
Claim · model-written summary
PVX titre was lower in H2B-silenced plants than non-silenced plants.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- H2B-silencede1
- Interactor
- PVXPotato virus Xe0pathogen
- Outcome
- titre of PVXe0
- Direction
- lowere0
- Comparator
- non-silenced plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there was a lower titre of PVX compared to non-silenced plants”
e1“H2B-silenced plants were inoculated with PVX”
t0“Nicotiana benthamiana”
Claim · model-written summary
H2B silencing produced abnormal leaf development and stem necrosis.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- H2B-silencede0
- Gene
- H2Be0
- Outcome
- Abnormal leaf development and stem necrosise0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Abnormal leaf development and stem necrosis were observed in the H2B-silenced plants”
t0“Nicotiana benthamiana”
Claim · model-written summary
Symptoms in H2B-silenced plants were alleviated in NahG transgenic plants.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Material
- NahG transgenic plantse0
- Treatment
- NahG transgenic plantse0
- Outcome
- Abnormal leaf development and stem necrosise1
- Direction
- alleviatede0
- Comparator
- H2B-silenced plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“which were alleviated in H2B-silenced NahG transgenic plants”
e1“Abnormal leaf development and stem necrosis were observed in the H2B-silenced plants”
t0“Nicotiana benthamiana”
Claim · model-written summary
H2B silencing increased endogenous SA in Nicotiana benthamiana.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- H2B-silencede0
- Gene
- H2Be0
- Outcome
- endogenous SAe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“endogenous SA is increased in H2B-silenced N. benthamiana”
Claim · model-written summary
SA accumulation correlated with stem necrosis and decreased PVX accumulation after H2B downregulation.
- Taxon
- N.benthamianaNicotiana benthamianae0
- Interactor
- PVXPotato virus Xe0pathogen
- Gene
- H2Be0
- Compound
- endogenous SAe0
- Outcome
- stem necrosis and a decreased accumulation of PVXe0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“downregulation of H2B induced accumulation of endogenous SA, which was correlated with stem necrosis and a decreased accumulation of PVX in N.benthamiana”
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See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.