Paper · 1986 · Weed Science

Alteration of Atrazine Uptake and Metabolism by Tridiphane in Giant Foxtail (Setaria faberi) and Corn (Zea mays)

Rick A. Boydston; Fred W. Slife

17
claims
21
verbatim quotes
21
citations
2
research areas
—
Not open access

Abstract

Partially purified glutathione- s -transferase (GST) isolated from corn ( Zea mays L.) seedlings was about 14-fold higher in specific activity than GST isolated from giant foxtail ( Setaria faberi Herrm. # SETFA) seedlings. Greater amounts of GST activity were present in leaf tissue than in stem tissue. Four-leaf giant foxtail seedlings contained about threefold more reduced glutathione per gram fresh weight in the leaves than one- to two-leaf giant foxtail seedlings. When atrazine and reduced glutathione were used as substrates, tridiphane [2-(3,5-dichlorophenyl)-2-(2,2,2-trichloroethyl)oxirane] inhibited isolated GST from corn with an I 50 of about 5 μ M and from giant foxtail with an I 50 slightly lower. Tridiphane inhibited the metabolism of atrazine [6-chloro- N -ethyl- N′ -(1-methylethyl)-1,3,5-triazine-2,4-diamine] to water-soluble metabolites in giant foxtail leaves but not in corn leaves. Unmetabolized atrazine levels tended to be greater in giant foxtail seedlings treated with tridiphane plus atrazine than in plants treated with atrazine alone. Tridiphane applied 12 h before atrazine increased the uptake of atrazine in both corn and five-leaf giant foxtail seedlings. The amount of atrazine metabolism to water-soluble metabolites increased in corn leaves but remained constant in giant foxtail leaves when atrazine uptake was increased. Tridiphane and atrazine combinations impaired net photosynthetic rates more than atrazine alone in giant foxtail seedlings but did not increase chlorophyll fluorescence. There was no decrease in net photosynthetic rate 12 h following applications of atrazine or tridiphane plus atrazine on corn leaves. Tridiphane did not decrease photosynthesis or increase chlorophyll fluorescence in either species when applied alone.

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Claims 17

Associationobservedoriginal resultmeasured

Claim · model-written summary

GST specific activity in corn was about 14-fold higher than in giant foxtail.

Gene
glutathione- s -transferasee1
Outcome
specific activitye1
Direction
highere1
Value
about 14-folde1
Comparator
GST isolated from giant foxtaile1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Partially purified glutathione- s -transferase (GST) isolated from corn ( Zea mays L.) seedlings was about 14-fold higher in specific activity than GST isolated from giant foxtail”

✓ located verbatim in the source · abstract, characters 0–179 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

GST activity was greater in leaf tissue than in stem tissue.

Organ / stage
leaf tissuee2
Outcome
GST activitye2
Direction
Greatere2
Comparator
stem tissuee2

Not reportedTaxon

Verbatim

e2

“Greater amounts of GST activity were present in leaf tissue than in stem tissue”

✓ located verbatim in the source · abstract, characters 224–303 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Four-leaf giant foxtail seedlings contained about threefold more reduced glutathione per gram fresh weight in leaves than one- to two-leaf seedlings.

Taxon
giant foxtaile3
Organ / stage
leavese3
Outcome
reduced glutathione per gram fresh weighte3
Direction
moree3
Value
about threefolde3
Comparator
one- to two-leaf giant foxtail seedlingse3

Verbatim

e3

“Four-leaf giant foxtail seedlings contained about threefold more reduced glutathione per gram fresh weight in the leaves than one- to two-leaf giant foxtail seedlings”

✓ located verbatim in the source · abstract, characters 305–471 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Tridiphane inhibited isolated GST from corn, with an I 50 of about 5 μ M.

Treatment
tridiphanee4
Outcome
I 50e4
Direction
inhibitede4
Value
about 5 μ Me4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e4

“tridiphane [2-(3,5-dichlorophenyl)-2-(2,2,2-trichloroethyl)oxirane] inhibited isolated GST from corn with an I 50 of about 5 μ M”

✓ located verbatim in the source · abstract, characters 536–664 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Tridiphane inhibited isolated GST from giant foxtail with an I 50 slightly lower than for corn.

Taxon
giant foxtaile5
Treatment
tridiphanee5
Outcome
I 50e5
Direction
inhibitede5
Value
slightly lowere5

Not reportedComparator

Verbatim

e5

“tridiphane [2-(3,5-dichlorophenyl)-2-(2,2,2-trichloroethyl)oxirane] inhibited isolated GST from corn with an I 50 of about 5 μ M and from giant foxtail with an I 50 slightly lower”

✓ located verbatim in the source · abstract, characters 536–715 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Unmetabolized atrazine levels tended to be greater in giant foxtail seedlings treated with tridiphane plus atrazine than in plants treated with atrazine alone.

Taxon
giant foxtaile7
Treatment
tridiphane plus atrazinee7
Outcome
Unmetabolized atrazine levelse7
Direction
tended to be greatere7
Comparator
atrazine alonee7

Verbatim

e7

“Unmetabolized atrazine levels tended to be greater in giant foxtail seedlings treated with tridiphane plus atrazine than in plants treated with atrazine alone”

✓ located verbatim in the source · abstract, characters 911–1069 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Tridiphane applied 12 h before atrazine increased the uptake of atrazine in corn.

Treatment
Tridiphane applied 12 h before atrazinee7
Outcome
uptake of atrazinee7
Direction
increasede7

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e7

“Tridiphane applied 12 h before atrazine increased the uptake of atrazine in both corn and five-leaf giant foxtail seedlings”

✓ located verbatim in the source · abstract, characters 1071–1194 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Tridiphane applied 12 h before atrazine increased the uptake of atrazine in five-leaf giant foxtail seedlings.

Taxon
giant foxtaile7
Organ / stage
five-leafe7
Treatment
Tridiphane applied 12 h before atrazinee7
Outcome
uptake of atrazinee7
Direction
increasede7

Not reportedComparator

Verbatim

e7

“Tridiphane applied 12 h before atrazine increased the uptake of atrazine in both corn and five-leaf giant foxtail seedlings”

✓ located verbatim in the source · abstract, characters 1071–1194 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Atrazine metabolism to water-soluble metabolites increased in corn leaves when atrazine uptake was increased.

Organ / stage
leavese7
Outcome
atrazine metabolism to water-soluble metabolitese7
Direction
increasede7
Qualifier
when atrazine uptake was increasede7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e7

“The amount of atrazine metabolism to water-soluble metabolites increased in corn leaves but remained constant in giant foxtail leaves when atrazine uptake was increased”

✓ located verbatim in the source · abstract, characters 1196–1364 · show in text ↑
Associationobservedoriginal resultno effectmeasured

Claim · model-written summary

Atrazine metabolism to water-soluble metabolites remained constant in giant foxtail leaves when atrazine uptake was increased.

Taxon
giant foxtaile7
Organ / stage
leavese7
Outcome
atrazine metabolism to water-soluble metabolitese7
Direction
remained constante7
Qualifier
when atrazine uptake was increasede7

Verbatim

e7

“The amount of atrazine metabolism to water-soluble metabolites increased in corn leaves but remained constant in giant foxtail leaves when atrazine uptake was increased”

✓ located verbatim in the source · abstract, characters 1196–1364 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Tridiphane and atrazine combinations impaired net photosynthetic rates in giant foxtail more than atrazine alone.

Taxon
giant foxtaile7
Treatment
Tridiphane and atrazine combinationse7
Outcome
net photosynthetic ratese7
Direction
impairede7
Comparator
atrazine alonee7

Verbatim

e7

“Tridiphane and atrazine combinations impaired net photosynthetic rates more than atrazine alone in giant foxtail seedlings but did not increase chlorophyll fluorescence”

✓ located verbatim in the source · abstract, characters 1366–1534 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Tridiphane and atrazine combinations did not increase chlorophyll fluorescence in giant foxtail.

Taxon
giant foxtaile7
Treatment
Tridiphane and atrazine combinationse7
Outcome
chlorophyll fluorescencee7
Direction
did not increasee7

Not reportedComparator

Verbatim

e7

“Tridiphane and atrazine combinations impaired net photosynthetic rates more than atrazine alone in giant foxtail seedlings but did not increase chlorophyll fluorescence”

✓ located verbatim in the source · abstract, characters 1366–1534 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

There was no decrease in net photosynthetic rate in corn leaves 12 h following applications of atrazine or tridiphane plus atrazine.

Organ / stage
leavese7
Treatment
applications of atrazine or tridiphane plus atrazinee7
Outcome
net photosynthetic ratee7
Direction
no decreasee7
Qualifier
12 h followinge7

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e7

“There was no decrease in net photosynthetic rate 12 h following applications of atrazine or tridiphane plus atrazine on corn leaves”

✓ located verbatim in the source · abstract, characters 1536–1667 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Tridiphane alone did not decrease photosynthesis in corn.

Treatment
Tridiphanee7
Outcome
photosynthesise7
Direction
did not decreasee7
Qualifier
when applied alonee7

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Giant Foxtail (Setaria faberi) and Corn (Zea mays)”

✓ located verbatim in the source · title, characters 62–112 · show in text ↑
e7

“Tridiphane did not decrease photosynthesis or increase chlorophyll fluorescence in either species when applied alone”

✓ located verbatim in the source · abstract, characters 1669–1785 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Tridiphane alone did not decrease photosynthesis in giant foxtail.

Taxon
Giant Foxtailt0
Treatment
Tridiphanee7
Outcome
photosynthesise7
Direction
did not decreasee7
Qualifier
when applied alonee7

Not reportedComparator

Verbatim

t0

“Giant Foxtail (Setaria faberi) and Corn (Zea mays)”

✓ located verbatim in the source · title, characters 62–112 · show in text ↑
e7

“Tridiphane did not decrease photosynthesis or increase chlorophyll fluorescence in either species when applied alone”

✓ located verbatim in the source · abstract, characters 1669–1785 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Tridiphane alone did not increase chlorophyll fluorescence in corn.

Treatment
Tridiphanee7
Outcome
chlorophyll fluorescencee7
Direction
no_changeinferrede7
Qualifier
when applied alonee7

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

t0

“Giant Foxtail (Setaria faberi) and Corn (Zea mays)”

✓ located verbatim in the source · title, characters 62–112 · show in text ↑
e7

“Tridiphane did not decrease photosynthesis or increase chlorophyll fluorescence in either species when applied alone”

✓ located verbatim in the source · abstract, characters 1669–1785 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Tridiphane alone did not increase chlorophyll fluorescence in giant foxtail.

Taxon
Giant Foxtailt0
Treatment
Tridiphanee7
Outcome
chlorophyll fluorescencee7
Direction
no_changeinferrede7
Qualifier
when applied alonee7

Not reportedComparator

Verbatim

t0

“Giant Foxtail (Setaria faberi) and Corn (Zea mays)”

✓ located verbatim in the source · title, characters 62–112 · show in text ↑
e7

“Tridiphane did not decrease photosynthesis or increase chlorophyll fluorescence in either species when applied alone”

✓ located verbatim in the source · abstract, characters 1669–1785 · show in text ↑

atrazine · physiology: photosynthesis · Zea mays (maize)Positive and negative results

5 papers: 2 positive, 3 negative or no effect

2 papers in the same direction as this paper · 2 in the opposite direction

  1. negative
  2. positive
  3. positive

1 paper more in this group

See all 5 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.