Abstract
Background The genetic regulation of flower color has been widely studied, notably as a character used by Mendel and his predecessors in the study of inheritance in pea. Methodology/Principal Findings We used the genome sequence of model legumes, together with their known synteny to the pea genome to identify candidate genes for the A and A2 loci in pea. We then used a combination of genetic mapping, fast neutron mutant analysis, allelic diversity, transcript quantification and transient expression complementation studies to confirm the identity of the candidates. Conclusions/Significance We have identified the pea genes A and A2. A is the factor determining anthocyanin pigmentation in pea that was used by Gregor Mendel 150 years ago in his study of inheritance. The A gene encodes a bHLH transcription factor. The white flowered mutant allele most likely used by Mendel is a simple G to A transition in a splice donor site that leads to a mis-spliced mRNA with a premature stop codon, and we have identified a second rare mutant allele. The A2 gene encodes a WD40 protein that is part of an evolutionarily conserved regulatory complex.
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Claims 6
Claim · model-written summary
The pea genes A and A2 were identified.
- Taxon
- pea
Lathyrus oleraceuse0 - Gene
- A and A2e0
- Outcome
- identified the pea genes A and A2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We have identified the pea genes A and A2.”
Claim · model-written summary
Pea A determines anthocyanin pigmentation.
- Taxon
- peaLathyrus oleraceuse0
- Gene
- Ae0
- Outcome
- anthocyanin pigmentatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A is the factor determining anthocyanin pigmentation in pea”
Claim · model-written summary
Pea A encodes a bHLH transcription factor.
Not reportedTaxon
Verbatim
e0“The A gene encodes a bHLH transcription factor.”
Claim · model-written summary
The likely Mendelian white-flower allele has a G-to-A splice-donor transition causing a mis-spliced mRNA and premature stop codon.
- Material
- white flowered mutant allelee0
- Outcome
- mis-spliced mRNA with a premature stop codone0
- Qualifier
- simple G to A transition in a splice donor sitee0
Not reportedTaxon
Verbatim
e0“The white flowered mutant allele most likely used by Mendel is a simple G to A transition in a splice donor site that leads to a mis-spliced mRNA with a premature stop codon”
Claim · model-written summary
A second rare mutant allele was identified.
Not reportedTaxon
Verbatim
e0“we have identified a second rare mutant allele.”
Claim · model-written summary
A2 encodes a WD40 protein in an evolutionarily conserved regulatory complex.
Not reportedTaxon
Verbatim
e0“The A2 gene encodes a WD40 protein that is part of an evolutionarily conserved regulatory complex.”