Abstract
Magnesium (Mg) deficiency is one of the major constraining factors that limit the yield and quality of agricultural products. Uniform seedlings of the Citrus sinensis were irrigated with Mg deficient (0 mM MgSO4) and Mg sufficient (1 mM MgSO4) nutrient solutions for 16 weeks. CO2 assimilation, starch, soluble carbohydrates, TBARS content and H2O2 production were measured. Transcriptomic analysis of C. sinensis leaves was performed by Illumina sequencing. Our results showed that Mg deficiency decreased CO2 assimilation, but increased starch, sucrose, TBARS content and H2O2 production in C. sinensis leaves. A total of 4864 genes showed differential expression in response to Mg deficiency revealed by RNA-Seq and the transcriptomic data were further validated by real-time quantitative PCR (RT-qPCR). Gene ontology (GO) enrichment analysis indicated that the mechanisms underlying Mg deficiency tolerance in C. sinensis may be attributed to the following aspects: (a) enhanced microtubule-based movement and cell cycle regulation; (b) elevated signal transduction in response to biotic and abiotic stimuli; (c) alteration of biological processes by tightly controlling phosphorylation especially protein phosphorylation; (d) down-regulation of light harvesting and photosynthesis due to the accumulation of carbohydrates; (e) up-regulation of cell wall remodeling and antioxidant system. Our results provide a comprehensive insight into the transcriptomic profile of key components involved in the Mg deficiency tolerance in C. sinensis and enrich our understanding of the molecular mechanisms by which plants adapted to a Mg deficient condition.
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Claims 9
Claim · model-written summary
Magnesium deficiency decreased CO2 assimilation.
- Taxon
- Citrus sinensis
Citrus sinensist0 - Treatment
- Mg deficiencye0
- Outcome
- CO2 assimilatione0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mg deficiency decreased CO2 assimilation”
t0“Citrus sinensis”
Claim · model-written summary
Magnesium deficiency increased starch and sucrose.
- Taxon
- Citrus sinensisCitrus sinensist0
- Treatment
- Mg deficiencye0
- Outcome
- starch, sucrosee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mg deficiency decreased CO2 assimilation, but increased starch, sucrose”
t0“Citrus sinensis”
Claim · model-written summary
Magnesium deficiency increased TBARS and H2O2.
- Taxon
- Citrus sinensisCitrus sinensist0
- Treatment
- Mg deficiencye0
- Outcome
- TBARS content and H2O2 productione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mg deficiency decreased CO2 assimilation, but increased starch, sucrose, TBARS content and H2O2 production”
t0“Citrus sinensis”
Claim · model-written summary
RNA-Seq identified 4864 differentially expressed genes under magnesium deficiency.
- Taxon
- Citrus sinensisCitrus sinensist0
- Outcome
- genes showed differential expressione0
- Value
- 4864 genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total of 4864 genes showed differential expression in response to Mg deficiency revealed by RNA-Seq”
t0“Citrus sinensis”
Claim · model-written summary
Enrichment indicated enhanced microtubule movement and cell cycle regulation.
- Taxon
- Citrus sinensisCitrus sinensist0
- Outcome
- microtubule-based movement and cell cycle regulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“enhanced microtubule-based movement and cell cycle regulation”
t0“Citrus sinensis”
Claim · model-written summary
Enrichment indicated elevated signal transduction.
- Taxon
- Citrus sinensisCitrus sinensist0
- Outcome
- signal transductione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“elevated signal transduction in response to biotic and abiotic stimuli”
t0“Citrus sinensis”
Claim · model-written summary
Enrichment indicated altered phosphorylation.
- Taxon
- Citrus sinensisCitrus sinensist0
- Outcome
- phosphorylatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“alteration of biological processes by tightly controlling phosphorylation especially protein phosphorylation”
t0“Citrus sinensis”
Claim · model-written summary
Enrichment indicated down-regulated light harvesting and photosynthesis.
- Taxon
- Citrus sinensisCitrus sinensist0
- Outcome
- light harvesting and photosynthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“down-regulation of light harvesting and photosynthesis due to the accumulation of carbohydrates”
t0“Citrus sinensis”
Claim · model-written summary
Enrichment indicated up-regulated cell wall remodeling and antioxidant system.
- Taxon
- Citrus sinensisCitrus sinensist0
- Outcome
- cell wall remodeling and antioxidant systeme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“up-regulation of cell wall remodeling and antioxidant system”
t0“Citrus sinensis”
The same questions in other papers
magnesium atom · physiology: photosynthesis · Citrus sinensis (sweet orange)Results agree
3 papers in the same direction as this paper
This paper is about lacking the nutrient, so it counts the other way round here.
- positive
- positive
- positive
magnesium atom · quality: sugar starch · Citrus sinensis (sweet orange)Results agree
3 papers in the same direction as this paper
This paper is about lacking the nutrient, so it counts the other way round here.
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.