Abstract
This study was conducted to isolate and molecularly identify two pathogenic fungi namely Rhizoctonia solani and Fusarium solani implicated in root rot and seedling damping-off disease of cucumber, Cucumis sativus. Besides, the efficacy of Pseudomonas fluorescens and Bacillus subtilis as bacterial agents in controlling these two pathogens was also evaluated in vitro and in a greenhouse pot experiment. Results of polymerase chain reaction (PCR) amplification and nucleotide sequence analysis using BLAST demonstrated that R. solani isolate was genetically different from the R. solani isolates in the National Centre for Biotechnology Information (NCBI). Therefore, it was recorded in GenBank under the accession number MK105921. P. fluorescens and B. subtilis showed a complete inhibition of the mycelial growths of R. solani and F. solani in vitro. In the pot experiments, soil treatment with a suspension of P. fluorescens and B. subtilis before planting significantly reduced the damping off of cucumber seedlings caused by R. solani and F. solani. This study suggests that these bacterial antagonists could have a good potential as biological control agents to protect cucumber plants from the infection with R. solani and F. solani.
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Claims 9
Claim · model-written summary
The R. solani isolate differed genetically from database isolates.
- Taxon
- cucumber
Cucumis sativuse1 - Interactor
- R. solani
Rhizoctonia solanie0pathogen - Outcome
- genetically differente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“R. solani isolate was genetically different from the R. solani isolates in the National Centre for Biotechnology Information (NCBI)”
e1“root rot and seedling damping-off disease of cucumber, Cucumis sativus”
Claim · model-written summary
The R. solani isolate was deposited under accession MK105921.
- Taxon
- cucumberCucumis sativuse2
- Interactor
- R. solaniRhizoctonia solanie0pathogen
- Outcome
- recorded in GenBanke1
- Value
- MK105921e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“R. solani isolate was genetically different from the R. solani isolates in the National Centre for Biotechnology Information (NCBI)”
e1“it was recorded in GenBank under the accession number MK105921”
e2“root rot and seedling damping-off disease of cucumber, Cucumis sativus”
Claim · model-written summary
P. fluorescens completely inhibited R. solani mycelial growth in vitro.
- Taxon
- cucumberCucumis sativuse1
- Treatment
- P. fluorescense0
- Interactor
- R. solaniRhizoctonia solanie0pathogen
- Second interactor
- P. fluorescens
.JPG?width=60)
Pseudomonas fluorescense0biocontrol_agent - Outcome
- mycelial growths of R. solanie0
- Direction
- complete inhibitione0
- Setting
- in vitroe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.JPG?width=500)
Verbatim
e0“P. fluorescens and B. subtilis showed a complete inhibition of the mycelial growths of R. solani and F. solani in vitro”
e1“root rot and seedling damping-off disease of cucumber, Cucumis sativus”
Claim · model-written summary
P. fluorescens completely inhibited F. solani mycelial growth in vitro.
- Taxon
- cucumberCucumis sativuse1
- Treatment
- P. fluorescense0
- Interactor
- F. solani
_Sacc._5393379.jpg?width=60)
Fusarium solanie0pathogen - Second interactor
- P. fluorescensPseudomonas fluorescense0biocontrol_agent
- Outcome
- mycelial growths of R. solani and F. solanie0
- Direction
- complete inhibitione0
- Setting
- in vitroe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Sacc._5393379.jpg?width=500)
Verbatim
e0“P. fluorescens and B. subtilis showed a complete inhibition of the mycelial growths of R. solani and F. solani in vitro”
e1“root rot and seedling damping-off disease of cucumber, Cucumis sativus”
Claim · model-written summary
B. subtilis completely inhibited R. solani mycelial growth in vitro.
- Taxon
- cucumberCucumis sativuse1
- Treatment
- B. subtilise0
- Interactor
- R. solaniRhizoctonia solanie0pathogen
- Second interactor
- B. subtilis
Bacillus subtilise0biocontrol_agent - Outcome
- mycelial growths of R. solanie0
- Direction
- complete inhibitione0
- Setting
- in vitroe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“P. fluorescens and B. subtilis showed a complete inhibition of the mycelial growths of R. solani and F. solani in vitro”
e1“root rot and seedling damping-off disease of cucumber, Cucumis sativus”
Claim · model-written summary
B. subtilis completely inhibited F. solani mycelial growth in vitro.
- Taxon
- cucumberCucumis sativuse1
- Treatment
- B. subtilise0
- Interactor
- F. solaniFusarium solanie0pathogen
- Second interactor
- B. subtilisBacillus subtilise0biocontrol_agent
- Outcome
- mycelial growths of R. solani and F. solanie0
- Direction
- complete inhibitione0
- Setting
- in vitroe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“P. fluorescens and B. subtilis showed a complete inhibition of the mycelial growths of R. solani and F. solani in vitro”
e1“root rot and seedling damping-off disease of cucumber, Cucumis sativus”
Claim · model-written summary
Preplant soil treatment with P. fluorescens significantly reduced cucumber seedling damping off caused by R. solani.
- Taxon
- cucumberCucumis sativuse0
- Treatment
- soil treatment with a suspension of P. fluorescens and B. subtilis before plantinge0
- Interactor
- R. solaniRhizoctonia solanie0pathogen
- Second interactor
- P. fluorescensPseudomonas fluorescense0biocontrol_agent
- Outcome
- damping off of cucumber seedlingse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soil treatment with a suspension of P. fluorescens and B. subtilis before planting significantly reduced the damping off of cucumber seedlings caused by R. solani and F. solani”
Claim · model-written summary
Preplant soil treatment with B. subtilis significantly reduced cucumber seedling damping off caused by F. solani.
- Taxon
- cucumberCucumis sativuse0
- Treatment
- soil treatment with a suspension of P. fluorescens and B. subtilis before plantinge0
- Interactor
- F. solaniFusarium solanie0pathogen
- Second interactor
- B. subtilisBacillus subtilise0biocontrol_agent
- Outcome
- damping off of cucumber seedlingse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soil treatment with a suspension of P. fluorescens and B. subtilis before planting significantly reduced the damping off of cucumber seedlings caused by R. solani and F. solani”
Claim · model-written summary
The bacterial antagonists were suggested as potential biological controls to protect cucumber from infection.
- Taxon
- cucumberCucumis sativuse0
- Interactor
- R. solaniRhizoctonia solanie0pathogen
- Outcome
- biological control agents to protect cucumber plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these bacterial antagonists could have a good potential as biological control agents to protect cucumber plants from the infection with R. solani and F. solani”
The same questions in other papers
Pseudomonas fluorescens · Fusarium solani · Cucumis sativus (cucumber)Results agree
1 paper in the same direction as this paper
- positive
Bacillus subtilis · Rhizoctonia solani · Cucumis sativus (cucumber)Results agree
2 papers in the same direction as this paper
- positive
- positive
Pseudomonas fluorescens · Rhizoctonia solani · Cucumis sativus (cucumber)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.