Abstract
This comprehensive survey of Xyloglucan endotransglucosylase/hydrolase (XTH) and functional validation of citrus XTH genes provides new insights into the involvement of XTHs in Xanthomonas citri subsp. citri (Xcc) infection. In this study, from the genome of sweet orange, a total of 34 CsXTH genes with XTH characteristic domains were identified and clustered into groups I/II, IIIA and IIIB. Except chromosome 9, the CsXTH genes were unevenly distributed and duplicated among all chromosomes which made CsXTHs hotpot on chromosome 4. With Xcc induction, a group of citrus canker related CsXTHs were detected. To explored the relations between CsXTHs and citrus bacterial canker (CBC), we explored the role of CsXTH04, was identified as a putative candidate gene which can be up-regulated in CBC-resistant variety. CsXTH04 verified to be a secret protein and can be induced by exogenous hormones salicylic acid (SA) and methyl jasmonate (MeJA). Over-expression of CsXTH04 confers marked CBC susceptibility to transgenic citrus, whereas the silencing of CsWAKL08 confers CBC resistance. Over all, the expert annotation of CsXTH family provides an initial basis for the functional and evolutionary study of this family while the potential CBC susceptible gene CsXTH04, validated in this study, can be used in citrus breeding to improve the CBC resistance.
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Claims 10
Claim · model-written summary
Sweet orange has 34 identified CsXTH genes with characteristic domains, clustered into three groups.
- Taxon
- sweet orange
Citrus sinensise0 - Gene
- CsXTHe0
- Outcome
- genes with XTH characteristic domainse0
- Value
- 34e0
- Qualifier
- groups I/II, IIIA and IIIBe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“from the genome of sweet orange, a total of 34 CsXTH genes with XTH characteristic domains were identified and clustered into groups I/II, IIIA and IIIB”
Claim · model-written summary
CsXTH genes were unevenly distributed and duplicated across chromosomes except chromosome 9, with a hotspot on chromosome 4.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Except chromosome 9, the CsXTH genes were unevenly distributed and duplicated among all chromosomes which made CsXTHs hotpot on chromosome 4.”
t0“Citrus XTH Genes”
Claim · model-written summary
Xcc induction revealed a group of citrus canker-related CsXTHs.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“With Xcc induction, a group of citrus canker related CsXTHs were detected.”
Claim · model-written summary
CsXTH04 was identified as a candidate gene up-regulated in a CBC-resistant variety.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we explored the role of CsXTH04, was identified as a putative candidate gene which can be up-regulated in CBC-resistant variety.”
t0“Citrus XTH Genes”
Claim · model-written summary
CsXTH04 was verified to be a secret protein.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CsXTH04 verified to be a secret protein”
t0“Citrus XTH Genes”
Claim · model-written summary
Exogenous salicylic acid can induce CsXTH04.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CsXTH04 verified to be a secret protein and can be induced by exogenous hormones salicylic acid (SA) and methyl jasmonate (MeJA).”
t0“Citrus XTH Genes”
Claim · model-written summary
Exogenous methyl jasmonate can induce CsXTH04.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CsXTH04 verified to be a secret protein and can be induced by exogenous hormones salicylic acid (SA) and methyl jasmonate (MeJA).”
t0“Citrus XTH Genes”
Claim · model-written summary
CsXTH04 over-expression confers marked CBC susceptibility in transgenic citrus.
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Over-expression of CsXTH04 confers marked CBC susceptibility to transgenic citrus”
Claim · model-written summary
Silencing CsWAKL08 confers CBC resistance.
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“whereas the silencing of CsWAKL08 confers CBC resistance.”
t0“Citrus XTH Genes”
Claim · model-written summary
The authors propose using CsXTH04 in citrus breeding to improve CBC resistance.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the potential CBC susceptible gene CsXTH04, validated in this study, can be used in citrus breeding to improve the CBC resistance.”
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