Abstract
Cytokinins are one of the most important phytohormones and play essential roles in multiple life processes in planta. Root-derived cytokinins are transported to the shoots via long-distance transport. The mechanisms of long-distance transport of root-derived cytokinins remain to be demonstrated. In this study, we report that OsABCG18, a half-size ATP-binding cassette transporter from rice (Oryza sativa L.), is essential for the long-distance transport of root-derived cytokinins. OsABCG18 encodes a plasma membrane protein and is primarily expressed in the vascular tissues of the root, stem, and leaf midribs. Cytokinin profiling, as well as [14C]trans-zeatin tracer, and xylem sap assays, demonstrated that the shootward transport of root-derived cytokinins was significantly suppressed in the osabcg18 mutants. Transport assays in tobacco (Nicotiana benthamiana) indicated that OsABCG18 exhibited efflux transport activities for various substrates of cytokinins. While the mutation reduced root-derived cytokinins in the shoot and grain yield, overexpression of OsABCG18 significantly increased cytokinins in the shoot and improved grain yield. The findings for OsABCG18 as a transporter for long-distance transport of cytokinin provide new insights into the cytokinin transport mechanism and a novel strategy to increase cytokinins in the shoot and promote grain yield.
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Claims 6
Claim · model-written summary
The shootward transport of root-derived cytokinins was significantly suppressed in osabcg18 mutants.
- Taxon
- rice
Oryza sativat0 - Material
- osabcg18 mutantse0
- Treatment
- osabcg18 mutantse0
- Gene
- OsABCG18e1
- Outcome
- shootward transport of root-derived cytokininse0
- Direction
- significantly suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cytokinins and grain yield in rice”
e0“the shootward transport of root-derived cytokinins was significantly suppressed in the osabcg18 mutants”
e1“OsABCG18, a half-size ATP-binding cassette transporter from rice (Oryza sativa L.), is essential for the long-distance transport of root-derived cytokinins”
Claim · model-written summary
OsABCG18 is primarily expressed in the vascular tissues of the root, stem and leaf midribs.
- Taxon
- riceOryza sativat0
- Organ / stage
- vascular tissues of the root, stem, and leaf midribse2
- Gene
- OsABCG18e2
- Outcome
- primarily expressede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cytokinins and grain yield in rice”
e2“OsABCG18 encodes a plasma membrane protein and is primarily expressed in the vascular tissues of the root, stem, and leaf midribs”
Claim · model-written summary
OsABCG18 exhibited efflux transport activity for various cytokinin substrates in tobacco assays.
- Taxon
- riceOryza sativat0
- Gene
- OsABCG18e4
- Outcome
- efflux transport activitiese4
- Setting
- tobaccoe4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cytokinins and grain yield in rice”
e4“Transport assays in tobacco (Nicotiana benthamiana) indicated that OsABCG18 exhibited efflux transport activities for various substrates of cytokinins”
Claim · model-written summary
The mutation reduced root-derived cytokinins in the shoot and grain yield.
- Taxon
- riceOryza sativat0
- Treatment
- mutatione5
- Outcome
- root-derived cytokinins in the shoot and grain yielde5
- Direction
- reducede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cytokinins and grain yield in rice”
e5“While the mutation reduced root-derived cytokinins in the shoot and grain yield”
Claim · model-written summary
Overexpression of OsABCG18 significantly increased cytokinins in the shoot.
- Taxon
- riceOryza sativat0
- Treatment
- overexpression of OsABCG18e6
- Outcome
- cytokinins in the shoote6
- Direction
- significantly increasede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cytokinins and grain yield in rice”
e6“overexpression of OsABCG18 significantly increased cytokinins in the shoot and improved grain yield”
Claim · model-written summary
Overexpression of OsABCG18 improved grain yield.
- Taxon
- riceOryza sativat0
- Treatment
- overexpression of OsABCG18e6
- Outcome
- grain yielde6
- Direction
- improvede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cytokinins and grain yield in rice”
e6“overexpression of OsABCG18 significantly increased cytokinins in the shoot and improved grain yield”
The same questions in other papers
loss of function · physiology: hormones · Oryza sativa (rice)Positive and negative results
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