Abstract
The mediator complex is an essential link between transcription factors and RNA polymerase II, and mainly functions in the transduction of diverse signals to genes involved in different pathways. Limited information is available on the role of soybean mediator subunits in growth and development, and their participation in defense response regulation. Here, we performed genome-wide identification of the 95 soybean mediator subunits, which were unevenly localized on the 20 chromosomes and only segmental duplication events were detected. We focused on GmMED16-1, which is highly expressed in the roots, for further functional analysis. Transcription of GmMED16-1 was induced in response to Phytophthora sojae infection. Agrobacterium rhizogenes mediated soybean hairy root transformation was performed for the silencing of the GmMED16-1 gene. Silencing of GmMED16-1 led to an enhanced susceptibility phenotype and increased accumulation of P. sojae biomass in hairy roots of transformants. The transcript levels of NPR1, PR1a, and PR5 in the salicylic acid defense pathway in roots of GmMED16-1-silenced transformants were lower than those of empty-vector transformants. The results provide evidence that GmMED16-1 may participate in the soybean–P. sojae interaction via a salicylic acid-dependent process.
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Claims 9
Claim · model-written summary
Ninety-five soybean mediator subunits were identified.
- Taxon
- soybean
Glycine maxe0 - Outcome
- soybean mediator subunitse0
- Value
- 95e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“genome-wide identification of the 95 soybean mediator subunits”
Claim · model-written summary
Soybean mediator subunits were unevenly located on 20 chromosomes.
- Taxon
- soybeanGlycine maxe0
- Outcome
- unevenly localized on the 20 chromosomese0
- Value
- 20 chromosomese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the 95 soybean mediator subunits, which were unevenly localized on the 20 chromosomes”
Claim · model-written summary
Only segmental duplication events were detected for soybean mediator subunits.
- Taxon
- soybeanGlycine maxe0
- Outcome
- only segmental duplication events were detectede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soybean mediator subunits, which were unevenly localized on the 20 chromosomes and only segmental duplication events were detected”
Claim · model-written summary
GmMED16-1 was highly expressed in roots.
Not reportedTaxon
Verbatim
e0“GmMED16-1, which is highly expressed in the roots”
Claim · model-written summary
Phytophthora sojae infection induced GmMED16-1 transcription.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- Phytophthora sojae infectione0
- Interactor
- Phytophthora sojaePhytophthora sojaee0pathogen
- Gene
- GmMED16-1e0
- Outcome
- Transcription of GmMED16-1e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcription of GmMED16-1 was induced in response to Phytophthora sojae infection.”
t0“Soybean—Phytophthora sojae Interaction”
Claim · model-written summary
GmMED16-1 silencing enhanced susceptibility in soybean hairy roots.
- Taxon
- SoybeanGlycine maxt0
- Organ / stage
- hairy rootse0
- Treatment
- Silencing of GmMED16-1e0
- Interactor
- P. sojaePhytophthora sojaee0pathogen
- Gene
- GmMED16-1e0
- Outcome
- susceptibility phenotypee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of GmMED16-1 led to an enhanced susceptibility phenotype and increased accumulation of P. sojae biomass in hairy roots of transformants.”
t0“Soybean—Phytophthora sojae Interaction”
Claim · model-written summary
GmMED16-1 silencing increased P. sojae biomass in hairy roots.
- Taxon
- SoybeanGlycine maxt0
- Organ / stage
- hairy rootse0
- Treatment
- Silencing of GmMED16-1e0
- Interactor
- P. sojaePhytophthora sojaee0pathogen
- Gene
- GmMED16-1e0
- Outcome
- P. sojae biomasse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of GmMED16-1 led to an enhanced susceptibility phenotype and increased accumulation of P. sojae biomass in hairy roots of transformants.”
t0“Soybean—Phytophthora sojae Interaction”
Claim · model-written summary
GmMED16-1-silenced roots had lower NPR1, PR1a, and PR5 transcript levels than empty-vector roots.
- Taxon
- SoybeanGlycine maxt0
- Organ / stage
- rootse0
- Treatment
- GmMED16-1-silenced transformantse0
- Gene
- NPR1, PR1a, and PR5e0
- Outcome
- transcript levelse0
- Direction
- lowere0
- Comparator
- empty-vector transformantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcript levels of NPR1, PR1a, and PR5 in the salicylic acid defense pathway in roots of GmMED16-1-silenced transformants were lower than those of empty-vector transformants.”
t0“Soybean—Phytophthora sojae Interaction”
Claim · model-written summary
The authors infer that GmMED16-1 participates in the soybean–P. sojae interaction through a salicylic acid-dependent process.
- Taxon
- soybeanGlycine maxe0
- Interactor
- P. sojaePhytophthora sojaee0pathogen
- Gene
- GmMED16-1e0
- Outcome
- soybean–P. sojae interactione0
- Qualifier
- salicylic acid-dependent processe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmMED16-1 may participate in the soybean–P. sojae interaction via a salicylic acid-dependent process.”
The same questions in other papers
loss of function · Phytophthora sojae · Glycine max (soybean)Positive and negative results
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