Abstract
Phytophthora sojae Kaufmann and Gerdemann causes Phytophthora root rot, a destructive soybean disease worldwide. A basic helix-loop-helix (bHLH) transcription factor is thought to be involved in the response to P. sojae infection in soybean, as revealed by RNA sequencing (RNA-seq). However, the molecular mechanism underlying this response is currently unclear. Here, we explored the function and underlying mechanisms of a bHLH transcription factor in soybean, designated GmPIB1 (P. sojae-inducible bHLH transcription factor), during host responses to P. sojae. GmPIB1 was significantly induced by P. sojae in the resistant soybean cultivar 'L77-1863'. Analysis of transgenic soybean hairy roots with elevated or reduced expression of GmPIB1 demonstrated that GmPIB1 enhances resistance to P. sojae and reduces reactive oxygen species (ROS) accumulation. Quantitative reverse transcription PCR and chromatin immunoprecipitation-quantitative PCR assays revealed that GmPIB1 binds directly to the promoter of GmSPOD1 and represses its expression; this gene encodes a key enzyme in ROS production. Moreover, transgenic soybean hairy roots with GmSPOD1 silencing through RNA interference exhibited improved resistance to P. sojae and reduced ROS generation. These findings suggest that GmPIB1 enhances resistance to P. sojae by repressing the expression of GmSPOD1.
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Claims 8
Claim · model-written summary
P. sojae significantly induced GmPIB1 in resistant soybean cultivar L77-1863.
- Taxon
- Glycine max
Glycine maxt0 - Material
- 'L77-1863'e0
- Interactor
- P. sojaePhytophthora sojaee0pathogen
- Gene
- GmPIB1e0
- Outcome
- GmPIB1e0
- Direction
- inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmPIB1 was significantly induced by P. sojae in the resistant soybean cultivar 'L77-1863'.”
t0“Glycine max”
Claim · model-written summary
Elevated GmPIB1 expression enhanced resistance to P. sojae.
- Taxon
- Glycine maxGlycine maxt0
- Treatment
- GmPIB1e0
- Interactor
- P. sojaePhytophthora sojaee0pathogen
- Gene
- GmPIB1e0
- Outcome
- resistance to P. sojaee0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmPIB1 enhances resistance to P. sojae and reduces reactive oxygen species (ROS) accumulation.”
t0“Glycine max”
Claim · model-written summary
Elevated GmPIB1 expression reduced ROS accumulation.
- Taxon
- Glycine maxGlycine maxt0
- Treatment
- GmPIB1e0
- Interactor
- P. sojaePhytophthora sojaee0pathogen
- Gene
- GmPIB1e0
- Outcome
- reactive oxygen species (ROS) accumulatione0
- Direction
- reducese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmPIB1 enhances resistance to P. sojae and reduces reactive oxygen species (ROS) accumulation.”
t0“Glycine max”
Claim · model-written summary
GmPIB1 directly binds the GmSPOD1 promoter.
- Taxon
- Glycine maxGlycine maxt0
- Gene
- GmPIB1e0
- Outcome
- binds directly to the promoter of GmSPOD1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmPIB1 binds directly to the promoter of GmSPOD1 and represses its expression; this gene encodes a key enzyme in ROS production.”
t0“Glycine max”
Claim · model-written summary
GmPIB1 represses GmSPOD1 expression.
- Taxon
- Glycine maxGlycine maxt0
- Gene
- GmSPOD1e0
- Outcome
- represses its expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GmPIB1 binds directly to the promoter of GmSPOD1 and represses its expression; this gene encodes a key enzyme in ROS production.”
t0“Glycine max”
Claim · model-written summary
GmSPOD1 encodes a key enzyme in ROS production.
- Taxon
- Glycine maxGlycine maxt0
- Outcome
- key enzyme in ROS productione0
- Qualifier
- ROS productione0
Not reportedGene
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this gene encodes a key enzyme in ROS production”
t0“Glycine max”
Claim · model-written summary
GmSPOD1 silencing improved resistance to P. sojae.
- Taxon
- Glycine maxGlycine maxt0
- Treatment
- GmSPOD1 silencing through RNA interferencee0
- Interactor
- P. sojaePhytophthora sojaee0pathogen
- Gene
- GmSPOD1e0
- Outcome
- resistance to P. sojaee0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenic soybean hairy roots with GmSPOD1 silencing through RNA interference exhibited improved resistance to P. sojae and reduced ROS generation.”
t0“Glycine max”
Claim · model-written summary
GmSPOD1 silencing reduced ROS generation.
- Taxon
- Glycine maxGlycine maxt0
- Treatment
- GmSPOD1 silencing through RNA interferencee0
- Interactor
- P. sojaePhytophthora sojaee0pathogen
- Gene
- GmSPOD1e0
- Outcome
- ROS generatione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenic soybean hairy roots with GmSPOD1 silencing through RNA interference exhibited improved resistance to P. sojae and reduced ROS generation.”
t0“Glycine max”
The same questions in other papers
loss of function · Phytophthora sojae · Glycine max (soybean)Positive and negative results
8 papers in the same direction as this paper · 15 in the opposite direction
- negative
- negative
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See all 24 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.