Abstract
L.), is a potent inhibitor of insect and animal herbivory and a neurostimulator of human brain function. Nicotine biosynthesis is controlled developmentally and can be induced by abiotic and biotic stressors via a jasmonic acid (JA)-mediated signal transduction mechanism involving members of the APETALA 2/ethylene-responsive factor (AP2/ERF) and basic helix-loop-helix (bHLH) transcription factor (TF) families. AP2/ERF and bHLH TFs work combinatorically to control nicotine biosynthesis and its subsequent accumulation in tobacco leaves. Here, we demonstrate that overexpression of the tobacco NtERF32, NtERF221/ORC1, and NtMYC2a TFs leads to significant increases in nicotine accumulation in T2 transgenic K326 tobacco plants before topping. Up to 9-fold higher nicotine production was achieved in transgenics overexpressing NtERF221/ORC1 under the control of a constitutive GmUBI3 gene promoter compared to wild-type plants. The constitutive 2XCaMV35S promoter and a novel JA-inducible 4XGAG promoter were less effective in driving high-level nicotine formation. Methyljasmonic acid (MeJA) treatment further elevated nicotine production in all transgenic lines. Our results show that targeted manipulation of NtERF221/ORC1 is an effective strategy for elevating leaf nicotine levels in commercial tobacco for use in the preparation of reduced risk tobacco products for smoking replacement therapeutics.
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Claims 8
Claim · model-written summary
Overexpression of NtERF32 significantly increased nicotine accumulation in transgenic K326 tobacco.
- Taxon
- Nicotiana tabacum
Nicotiana tabacumt0 - Material
- K326e1
- Treatment
- overexpressione1
- Gene
- NtERF32e1
- Outcome
- nicotine accumulatione1
- Direction
- increasese1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Flue-Cured Tobacco (Nicotiana tabacum L.)”
e1“overexpression of the tobacco NtERF32, NtERF221/ORC1, and NtMYC2a TFs leads to significant increases in nicotine accumulation in T2 transgenic K326 tobacco plants before topping”
Claim · model-written summary
Overexpression of NtERF221/ORC1 significantly increased nicotine accumulation in transgenic K326 tobacco.
- Taxon
- Nicotiana tabacumNicotiana tabacumt0
- Material
- K326e1
- Treatment
- overexpressione1
- Gene
- NtERF221/ORC1e1
- Outcome
- nicotine accumulatione1
- Direction
- increasese1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Flue-Cured Tobacco (Nicotiana tabacum L.)”
e1“overexpression of the tobacco NtERF32, NtERF221/ORC1, and NtMYC2a TFs leads to significant increases in nicotine accumulation in T2 transgenic K326 tobacco plants before topping”
Claim · model-written summary
Overexpression of NtMYC2a significantly increased nicotine accumulation in transgenic K326 tobacco.
- Taxon
- Nicotiana tabacumNicotiana tabacumt0
- Material
- K326e1
- Treatment
- overexpressione1
- Gene
- NtMYC2ae1
- Outcome
- nicotine accumulatione1
- Direction
- increasese1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Flue-Cured Tobacco (Nicotiana tabacum L.)”
e1“overexpression of the tobacco NtERF32, NtERF221/ORC1, and NtMYC2a TFs leads to significant increases in nicotine accumulation in T2 transgenic K326 tobacco plants before topping”
Claim · model-written summary
Overexpressing NtERF221/ORC1 under a constitutive GmUBI3 promoter gave up to 9-fold higher nicotine production than wild-type plants.
- Taxon
- Nicotiana tabacumNicotiana tabacumt0
- Treatment
- overexpressing NtERF221/ORC1 under the control of a constitutive GmUBI3 gene promotere2
- Gene
- NtERF221/ORC1e2
- Outcome
- nicotine productione2
- Direction
- highere2
- Value
- 9-folde2
- Comparator
- wild-type plantse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Flue-Cured Tobacco (Nicotiana tabacum L.)”
e2“Up to 9-fold higher nicotine production was achieved in transgenics overexpressing NtERF221/ORC1 under the control of a constitutive GmUBI3 gene promoter compared to wild-type plants”
Claim · model-written summary
The constitutive 2XCaMV35S promoter was less effective than other constructs in driving high-level nicotine formation.
- Taxon
- Nicotiana tabacumNicotiana tabacumt0
- Treatment
- constitutive 2XCaMV35S promotere3
- Outcome
- high-level nicotine formatione3
- Direction
- less effectivee3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Flue-Cured Tobacco (Nicotiana tabacum L.)”
e3“The constitutive 2XCaMV35S promoter and a novel JA-inducible 4XGAG promoter were less effective in driving high-level nicotine formation”
Claim · model-written summary
The JA-inducible 4XGAG promoter was less effective than other constructs in driving high-level nicotine formation.
- Taxon
- Nicotiana tabacumNicotiana tabacumt0
- Treatment
- novel JA-inducible 4XGAG promotere3
- Outcome
- high-level nicotine formatione3
- Direction
- less effectivee3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Flue-Cured Tobacco (Nicotiana tabacum L.)”
e3“The constitutive 2XCaMV35S promoter and a novel JA-inducible 4XGAG promoter were less effective in driving high-level nicotine formation”
Claim · model-written summary
Methyljasmonic acid (MeJA) treatment further elevated nicotine production in all transgenic tobacco lines.
- Taxon
- Nicotiana tabacumNicotiana tabacumt0
- Treatment
- Methyljasmonic acid (MeJA) treatmente4
- Outcome
- nicotine productione4
- Direction
- elevatede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Flue-Cured Tobacco (Nicotiana tabacum L.)”
e4“Methyljasmonic acid (MeJA) treatment further elevated nicotine production in all transgenic lines”
Claim · model-written summary
Targeted manipulation of NtERF221/ORC1 is an effective strategy for elevating leaf nicotine levels in commercial tobacco.
Verbatim
e5“targeted manipulation of NtERF221/ORC1 is an effective strategy for elevating leaf nicotine levels in commercial tobacco”
The same questions in other papers
jasmonic acid · molecular: metabolites · Nicotiana tabacum (tobacco)Positive and negative results
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- positive
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