Abstract
Jasmonate (JA), as an important signal, plays a key role in multiple processes of plant growth, development and stress response. Nicotine and related pyridine alkaloids in tobacco (Nicotiana tabacum L.) are essential secondary metabolites. Whether environmental factors control nicotine biosynthesis and the underlying mechanism remains previously unreported. Here, we applied physiological and biochemical approaches to investigate how salt stress affects nicotine biosynthesis in tobacco. We found that salt stress induced the biosynthesis of JA, which subsequently triggered the activation of JA-responsive gene expression and, ultimately, nicotine synthesis. Bioinformatics analysis revealed the existence of many NtMYC2a-recognized G-box motifs in the promoter regions of NtLOX, NtAOS, NtAOC and NtOPR genes. Applying exogenous JA increased nicotine content, while suppressing JA biosynthesis reduced nicotine biosynthesis. Salt treatment could not efficiently induce nicotine biosynthesis in transgenic anti-COI1 tobacco plants. These results demonstrate that JA acts as the essential signal which triggers nicotine biosynthesis in tobacco after salt stress.
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Claims 7
Claim · model-written summary
Salt stress induced jasmonate biosynthesis in tobacco.
- Taxon
- tobacco
Nicotiana tabacumt0 - Treatment
- salt stresse0
- Outcome
- biosynthesis of JAe0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“salt stress induced the biosynthesis of JA”
t0“tobacco”
Claim · model-written summary
Jasmonate subsequently activated JA-responsive gene expression.
- Taxon
- tobaccoNicotiana tabacumt0
- Compound
- JAe0
- Outcome
- JA-responsive gene expressione0
- Direction
- activatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“which subsequently triggered the activation of JA-responsive gene expression”
t0“tobacco”
Claim · model-written summary
Jasmonate signaling ultimately triggered nicotine synthesis after salt stress.
- Taxon
- tobaccoNicotiana tabacume1
- Compound
- JAe1
- Outcome
- nicotine biosynthesise1
- Direction
- triggerse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ultimately, nicotine synthesis”
e1“JA acts as the essential signal which triggers nicotine biosynthesis in tobacco after salt stress”
Claim · model-written summary
Promoters of NtLOX, NtAOS, NtAOC and NtOPR contain many NtMYC2a-recognized G-box motifs.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- NtLOX, NtAOS, NtAOC and NtOPRe0
- Outcome
- NtMYC2a-recognized G-box motifse0
- Qualifier
- manye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“many NtMYC2a-recognized G-box motifs in the promoter regions of NtLOX, NtAOS, NtAOC and NtOPR genes”
t0“tobacco”
Claim · model-written summary
Exogenous JA increased nicotine content in tobacco.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- exogenous JAe0
- Outcome
- nicotine contente0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Applying exogenous JA increased nicotine content”
t0“tobacco”
Claim · model-written summary
Suppressing JA biosynthesis reduced nicotine biosynthesis in tobacco.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- suppressing JA biosynthesise0
- Outcome
- nicotine biosynthesise0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suppressing JA biosynthesis reduced nicotine biosynthesis”
t0“tobacco”
Claim · model-written summary
Salt did not efficiently induce nicotine biosynthesis in transgenic anti-COI1 tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Material
- transgenic anti-COI1e0
- Treatment
- Salt treatmente0
- Outcome
- nicotine biosynthesise0
- Direction
- could not efficiently inducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Salt treatment could not efficiently induce nicotine biosynthesis in transgenic anti-COI1 tobacco plants”
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