Abstract
BACKGROUND: The ubiquitous signaling molecule melatonin (N-acetyl-5-methoxytryptamine) (MT) plays vital roles in plant development and stress tolerance. Selenium (Se) may be phytotoxic at high concentrations. Interactions between MT and Se (IV) stress in higher plants are poorly understood. The aim of this study was to evaluate the defensive roles of exogenous MT (0 μM, 50 μM, and 100 μM) against Se (IV) (0 μM, 50 μM, 100 μM, and 200 μM) stress based on the physiological and biochemical properties, thiol biosynthesis, and antioxidant system of Brassica napus plants subjected to these treatments. RESULTS: ) and PSII photochemical efficiency (Fv/Fm) in a dose-dependent manner. All of the aforementioned responses were effectively alleviated by exogenous MT treatment. Exogenous MT mitigated oxidative damage and lipid peroxidation and protected the plasma membranes from Se toxicity by reducing Se-induced reactive oxygen species (ROS) accumulation. MT also alleviated osmotic stress by restoring foliar water and sugar levels. Relative to standalone Se treatment, the combination of MT and Se upregulated the ROS-detoxifying enzymes SOD, APX, GR, and CAT, increased proline, free amino acids, and the thiol components GSH, GSSG, GSH/GSSG, NPTs, PCs, and cys and upregulated the metabolic enzymes γ-ECS, GST, and PCS. Therefore, MT application attenuates Se-induce oxidative damage in plants. MT promotes the accumulation of chelating agents in the roots, detoxifies Se there, and impedes its further translocation to the leaves. CONCLUSIONS: Exogenous MT improves the physiological traits, antioxidant system, and thiol ligand biosynthesis in B. napus subjected to Se stress primarily by enhancing Se detoxification and sequestration especially at the root level. Our results reveal better understanding of Se-phytotoxicity and Se-stress alleviation by the adequate supply of MT. The mechanisms of MT-induced plant tolerance to Se stress have potential implications in developing novel strategies for safe crop production in Se-rich soils.
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Claims 8
Claim · model-written summary
Melatonin alleviated the reported selenium-stress physiological responses.
- Taxon
- Brassica napus
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napuse1 - Treatment
- exogenous MT treatmente0
- Outcome
- aforementioned responsese0
- Direction
- alleviatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“All of the aforementioned responses were effectively alleviated by exogenous MT treatment.”
e1“Brassica napus plants subjected to these treatments”
Claim · model-written summary
Melatonin reduced oxidative damage and lipid peroxidation under selenium stress.
- Taxon
- Brassica napusBrassica napuse1
- Treatment
- Exogenous MTe0
- Outcome
- oxidative damage and lipid peroxidatione0
- Direction
- mitigatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exogenous MT mitigated oxidative damage and lipid peroxidation and protected the plasma membranes from Se toxicity by reducing Se-induced reactive oxygen species (ROS) accumulation.”
e1“Brassica napus plants”
Claim · model-written summary
Melatonin alleviated osmotic stress by restoring foliar water and sugar levels.
- Taxon
- Brassica napusBrassica napuse1
- Treatment
- MTe0
- Outcome
- osmotic stresse0
- Qualifier
- restoring foliar water and sugar levelse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MT also alleviated osmotic stress by restoring foliar water and sugar levels.”
e1“Brassica napus plants”
Claim · model-written summary
Combined melatonin and selenium upregulated ROS-detoxifying enzymes relative to selenium alone.
- Taxon
- Brassica napusBrassica napuse1
- Treatment
- combination of MT and See0
- Gene
- SOD, APX, GR, and CATe0
- Outcome
- ROS-detoxifying enzymes SOD, APX, GR, and CATe0
- Direction
- upregulatede0
- Comparator
- standalone Se treatmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Relative to standalone Se treatment, the combination of MT and Se upregulated the ROS-detoxifying enzymes SOD, APX, GR, and CAT”
e1“Brassica napus plants”
Claim · model-written summary
Combined melatonin and selenium increased proline and free amino acids relative to selenium alone.
- Taxon
- Brassica napusBrassica napuse1
- Treatment
- combination of MT and See0
- Outcome
- proline, free amino acidse0
- Direction
- increasede0
- Comparator
- standalone Se treatmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Relative to standalone Se treatment, the combination of MT and Se upregulated the ROS-detoxifying enzymes SOD, APX, GR, and CAT, increased proline, free amino acids, and the thiol components GSH, GSSG, GSH/GSSG, NPTs, PCs, and cys”
e1“Brassica napus plants”
Claim · model-written summary
Combined melatonin and selenium increased thiol components relative to selenium alone.
- Taxon
- Brassica napusBrassica napuse1
- Treatment
- combination of MT and See0
- Outcome
- thiol components GSH, GSSG, GSH/GSSG, NPTs, PCs, and cyse0
- Direction
- increasede0
- Comparator
- standalone Se treatmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Relative to standalone Se treatment, the combination of MT and Se upregulated the ROS-detoxifying enzymes SOD, APX, GR, and CAT, increased proline, free amino acids, and the thiol components GSH, GSSG, GSH/GSSG, NPTs, PCs, and cys”
e1“Brassica napus plants”
Claim · model-written summary
Combined melatonin and selenium upregulated γ-ECS, GST and PCS relative to selenium alone.
- Taxon
- Brassica napusBrassica napuse1
- Treatment
- combination of MT and See0
- Gene
- γ-ECS, GST, and PCSe0
- Outcome
- metabolic enzymes γ-ECS, GST, and PCSe0
- Direction
- upregulatede0
- Comparator
- standalone Se treatmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Relative to standalone Se treatment, the combination of MT and Se upregulated the ROS-detoxifying enzymes SOD, APX, GR, and CAT, increased proline, free amino acids, and the thiol components GSH, GSSG, GSH/GSSG, NPTs, PCs, and cys and upregulated the metabolic enzymes γ-ECS, GST, and PCS.”
e1“Brassica napus plants”
Claim · model-written summary
Melatonin promoted root chelator accumulation and selenium detoxification while impeding movement to leaves.
- Taxon
- Brassica napusBrassica napuse1
- Treatment
- MTe0
- Outcome
- Se there, and impedes its further translocation to the leavese0
- Qualifier
- accumulation of chelating agents in the rootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MT promotes the accumulation of chelating agents in the roots, detoxifies Se there, and impedes its further translocation to the leaves.”
e1“Brassica napus plants”
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See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.