Abstract
The callus transformation rate of Indica rice in tissue culture is relatively poor.Especially,the establishment of highly efficient regeneration system of superior varieties and the factors which affect its transformation remain unknown.This situation restricts at certain extent the progress of Indica rice genetic engineering.The seeds of restorer line Shuhui527 were used in this study to investigate the effects of quality and physiological state of theirs mature embryo callus under different temperatures and light conditions.Meanwhile,transformation efficiency influenced by differentiation medium supplemented with different hormones and the ratio between hormones were further studied.The results showed that under light condition at 32℃,the mature embryo callus growth is the best,under N6D medium callus quality is the best after twice subcultures.The NBS medium adding 2.0 mg/L KT+0.02 mg/L NAA+3 mg/L 6-BA+50 mg/L VC+0.5 mg/L IAA was found most suitable for Shuhui 527 genetic transformation,and the differentiation rate reached 36.42%.Using our new system,rice transcription factor OsbZIP39 was introduced into Indica rice genome by Agrobacterium-mediated transformation.Resistant plants were obtained preliminarily by PCR analysis.
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Claims 5
Claim · model-written summary
Mature embryo callus growth was best under light at 32℃.
- Taxon
- Indica rice
Oryza sativa Indica Groupt0 - Organ / stage
- mature embryo calluse0
- Treatment
- light condition at 32℃e0
- Outcome
- mature embryo callus growthe0
- Direction
- the beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“under light condition at 32℃,the mature embryo callus growth is the best”
t0“Indica Rice”
Claim · model-written summary
Callus quality was best on N6D medium after two subcultures.
- Taxon
- Indica RiceOryza sativa Indica Groupt0
- Treatment
- N6D mediume0
- Outcome
- callus qualitye0
- Direction
- the beste0
- Qualifier
- after twice subculturese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“under N6D medium callus quality is the best after twice subcultures”
t0“Indica Rice”
Claim · model-written summary
The specified NBS hormone medium was most suitable for Shuhui 527 transformation and gave 36.42% differentiation.
- Taxon
- Indica RiceOryza sativa Indica Groupt0
- Material
- Shuhui 527e0
- Treatment
- NBS medium adding 2.0 mg/L KT+0.02 mg/L NAA+3 mg/L 6-BA+50 mg/L VC+0.5 mg/L IAAe0
- Outcome
- differentiation ratee0
- Direction
- most suitablee0
- Value
- 36.42%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The NBS medium adding 2.0 mg/L KT+0.02 mg/L NAA+3 mg/L 6-BA+50 mg/L VC+0.5 mg/L IAA was found most suitable for Shuhui 527 genetic transformation,and the differentiation rate reached 36.42%”
t0“Indica Rice”
Claim · model-written summary
OsbZIP39 was introduced into the Indica rice genome with Agrobacterium transformation.
- Taxon
- Indica riceOryza sativa Indica Groupe0
- Treatment
- Agrobacterium-mediated transformatione0
- Gene
- OsbZIP39e0
- Outcome
- introduced into Indica rice genomee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rice transcription factor OsbZIP39 was introduced into Indica rice genome by Agrobacterium-mediated transformation”
Claim · model-written summary
PCR analysis preliminarily identified resistant plants.
- Taxon
- Indica RiceOryza sativa Indica Groupt0
- Outcome
- Resistant plants were obtainede0
- Qualifier
- preliminarily by PCR analysise0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Resistant plants were obtained preliminarily by PCR analysis”
t0“Indica Rice”
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