Paper · 2019 · IOP Conference Series Earth and Environmental Science

The Effect of Explant Types and Plant Growth Regulators On Callus Induction of Geranium (Pelargonium graveolens L’Her) In Vitro

Moch Faizul Huda; S Indriyani; W Widoretno

5
claims
10
verbatim quotes
1
citations
1
research areas
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Open access · cc-by

Abstract

Abstract The purpose of this study was to determine the effect of explant types and plant growth regulators on callus induction of geranium ( Pelargonium graveolens L’Her). Callus induction was carried out by culturing leaf and petiole explants from ex vitro geraniums on MS + 0.5 mg/l 2.4-D + 0.1 and 0.3 mg/l Benzyladenine (BA) or Kinetin. Each culture was carried out in 5 replications, cultures were incubated in rooms 25°C and 600 lux. The parameters days for callus induction and percentage of callus formation were observed. Geranium callus formation was influenced by types of explants and plant growth regulators. Petiole explants was able to induce callus better than leaf explants. Days for callus induction at second week and percentage of callus formation of petiole explants were 58 and 58% compared to leaf explants were only 53 and 54%. Plant Growth Regulators BA combined with 2.4-D was able to induce callus better than kinetin. The best callus formation was produced from petiole explants cultured on MS + 0.5 mg/l 2.4-D + 0.3 mg/l BA by 84%.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 5

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Petiole explants induced callus better than leaf explants in geranium.

Taxon
GeraniumGeraniume0
Treatment
petiole explantse1
Outcome
induce calluse1
Direction
bettere1
Comparator
leaf explantse1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Geranium
Geranium · Taxon · picture of the genus (one of its species)Alvesgaspar · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Geranium callus formation was influenced by types of explants and plant growth regulators”

✓ located verbatim in the source · abstract, characters 532–621 · show in text ↑
e1

“Petiole explants was able to induce callus better than leaf explants”

✓ located verbatim in the source · abstract, characters 623–691 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

BA combined with 2.4-D induced callus better than kinetin in geranium.

Taxon
GeraniumGeraniume0
Treatment
BA combined with 2.4-De2
Outcome
induce calluse2
Direction
bettere2
Comparator
kinetine2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Geranium
Geranium · Taxon · picture of the genus (one of its species)Alvesgaspar · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Geranium callus formation was influenced by types of explants and plant growth regulators”

✓ located verbatim in the source · abstract, characters 532–621 · show in text ↑
e2

“Plant Growth Regulators BA combined with 2.4-D was able to induce callus better than kinetin”

✓ located verbatim in the source · abstract, characters 853–945 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Petiole explants gave a higher percentage of callus formation (58%) than leaf explants in geranium.

Taxon
GeraniumGeraniume0
Treatment
petiole explantse3
Outcome
percentage of callus formatione3
Direction
higherinferrede3
Value
58%e3
Qualifier
53 and 54%e3
Comparator
leaf explantse3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Geranium
Geranium · Taxon · picture of the genus (one of its species)Alvesgaspar · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Geranium callus formation was influenced by types of explants and plant growth regulators”

✓ located verbatim in the source · abstract, characters 532–621 · show in text ↑
e3

“Days for callus induction at second week and percentage of callus formation of petiole explants were 58 and 58% compared to leaf explants were only 53 and 54%”

✓ located verbatim in the source · abstract, characters 693–851 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Petiole explants gave a higher number of days for callus induction at the second week (58) than leaf explants (53).

Taxon
GeraniumGeraniume0
Treatment
petiole explantse3
Outcome
Days for callus induction at second weeke3
Direction
higherinferrede3
Value
58e3
Qualifier
53e3
Comparator
leaf explantse3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Geranium
Geranium · Taxon · picture of the genus (one of its species)Alvesgaspar · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Geranium callus formation was influenced by types of explants and plant growth regulators”

✓ located verbatim in the source · abstract, characters 532–621 · show in text ↑
e3

“Days for callus induction at second week and percentage of callus formation of petiole explants were 58 and 58% compared to leaf explants were only 53 and 54%”

✓ located verbatim in the source · abstract, characters 693–851 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The best callus formation (84%) was from petiole explants cultured on MS + 0.5 mg/l 2.4-D + 0.3 mg/l BA.

Taxon
GeraniumGeraniume0
Organ / stage
petiole explantse4
Treatment
MS + 0.5 mg/l 2.4-D + 0.3 mg/l BAe4
Outcome
best callus formatione4
Value
84%e4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Geranium
Geranium · Taxon · picture of the genus (one of its species)Alvesgaspar · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Geranium callus formation was influenced by types of explants and plant growth regulators”

✓ located verbatim in the source · abstract, characters 532–621 · show in text ↑
e4

“The best callus formation was produced from petiole explants cultured on MS + 0.5 mg/l 2.4-D + 0.3 mg/l BA by 84%”

✓ located verbatim in the source · abstract, characters 947–1060 · show in text ↑

N-benzyladenine · growth · GeraniumResults agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

in vitro · growth · GeraniumPositive and negative results

6 papers: 4 positive, 2 negative or no effect

3 papers in the same direction as this paper · 2 in the opposite direction

  1. negative
  2. no effect
  3. positive

2 papers more in this group

See all 6 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.