Abstract
Polygalacturonase-inhibiting proteins (PGIPs) have been shown to recognize fungal polygalacturonases (PGs), which initiate innate immunity in various plant species. Notably, the connection between rice OsPGIPs and PGs in Xanthomonas oryzae pv. oryzicola (Xoc), which causes bacterial leaf streak (BLS), remains unclear. Here, we show that OsPGIP1 was strongly induced after inoculating rice with the Xoc strain RS105. Furthermore, OsPGIP1-overexpressing (OV) and RNA interference (RNAi) rice lines increased and decreased, respectively, the resistance of rice to RS105, indicating that OsPGIP1 contributes to BLS resistance. Subsequently, we generated the unique PG mutant RS105Δpg, the virulence of which is attenuated compared to that of RS105. Surprisingly, the lesion lengths caused by RS105Δpg were similar to those caused by RS105 in the OV lines compared with wild-type ZH11 with reduced Xoc susceptibility. However, the lesion lengths caused by RS105Δpg were still significantly shorter in the OV lines than in ZH11, implying that OsPGIP1-mediated BLS resistance could respond to other virulence factors in addition to PGs. To explore the OsPGIP1-mediated resistance, RNA-seq analysis were performed and showed that many plant cell wall-associated genes and several MYB transcription factor genes were specifically expressed or more highly induced in the OV lines compared to ZH11 postinoculation with RS105. Consistent with the expression of the differentially expressed genes, the OV plants accumulated a higher content of jasmonic acid (JA) than ZH11 postinoculation with RS105, suggesting that the OsPGIP1-mediated resistance to BLS is mainly dependent on the plant cell wall-associated immunity and the JA signaling pathway.
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Claims 9
Claim · model-written summary
OsPGIP1 was strongly induced after RS105 inoculation.
- Taxon
- Rice
Oryza sativat0 - Treatment
- inoculating rice with the Xoc strain RS105e0
- Interactor
- Xoc strain RS105e0pathogen
- Gene
- OsPGIP1e0
- Outcome
- OsPGIP1e0
- Direction
- strongly inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“OsPGIP1 was strongly induced after inoculating rice with the Xoc strain RS105”
Claim · model-written summary
OsPGIP1 overexpression increased rice resistance to RS105.
- Taxon
- RiceOryza sativat0
- Treatment
- OsPGIP1-overexpressing (OV)e0
- Interactor
- RS105e0pathogen
- Gene
- OsPGIP1e0
- Outcome
- resistance of rice to RS105e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“OsPGIP1-overexpressing (OV) and RNA interference (RNAi) rice lines increased and decreased, respectively, the resistance of rice to RS105”
Claim · model-written summary
OsPGIP1 RNA interference decreased rice resistance to RS105.
- Taxon
- RiceOryza sativat0
- Treatment
- RNA interference (RNAi)e0
- Interactor
- RS105e0pathogen
- Gene
- OsPGIP1e0
- Outcome
- resistance of rice to RS105e0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“OsPGIP1-overexpressing (OV) and RNA interference (RNAi) rice lines increased and decreased, respectively, the resistance of rice to RS105”
Claim · model-written summary
The PG mutant RS105Δpg had attenuated virulence relative to RS105.
- Taxon
- RiceOryza sativat0
- Interactor
- RS105Δpge0pathogen
- Outcome
- virulencee0
- Direction
- attenuatede0
- Comparator
- RS105e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“PG mutant RS105Δpg, the virulence of which is attenuated compared to that of RS105”
Claim · model-written summary
Lesions from RS105Δpg and RS105 were similar in OV lines.
- Taxon
- RiceOryza sativat0
- Material
- OV linese0
- Interactor
- RS105Δpge0pathogen
- Outcome
- lesion lengthse0
- Direction
- similare0
- Comparator
- RS105e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“lesion lengths caused by RS105Δpg were similar to those caused by RS105 in the OV lines”
Claim · model-written summary
RS105Δpg caused significantly shorter lesions in OV lines than ZH11.
- Taxon
- RiceOryza sativat0
- Material
- OV linese0
- Interactor
- RS105Δpge0pathogen
- Outcome
- lesion lengthse0
- Direction
- shortere0
- Comparator
- ZH11e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“lesion lengths caused by RS105Δpg were still significantly shorter in the OV lines than in ZH11”
Claim · model-written summary
Cell wall-associated genes were specifically or more highly expressed in OV lines after RS105 inoculation.
- Taxon
- RiceOryza sativat0
- Material
- OV linese0
- Interactor
- RS105e0pathogen
- Gene
- plant cell wall-associated genese0
- Outcome
- plant cell wall-associated genese0
- Direction
- more highly inducede0
- Comparator
- ZH11e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“many plant cell wall-associated genes and several MYB transcription factor genes were specifically expressed or more highly induced in the OV lines compared to ZH11 postinoculation with RS105”
Claim · model-written summary
MYB transcription factor genes were specifically or more highly expressed in OV lines after RS105 inoculation.
- Taxon
- RiceOryza sativat0
- Material
- OV linese0
- Interactor
- RS105e0pathogen
- Gene
- MYB transcription factor genese0
- Outcome
- MYB transcription factor genese0
- Direction
- more highly inducede0
- Comparator
- ZH11e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“several MYB transcription factor genes were specifically expressed or more highly induced in the OV lines compared to ZH11 postinoculation with RS105”
Claim · model-written summary
OV plants accumulated more jasmonic acid than ZH11 after RS105 inoculation.
- Taxon
- RiceOryza sativat0
- Material
- OV plantse0
- Interactor
- RS105e0pathogen
- Outcome
- content of jasmonic acid (JA)e0
- Direction
- highere0
- Comparator
- ZH11e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“OV plants accumulated a higher content of jasmonic acid (JA) than ZH11 postinoculation with RS105”
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