Abstract
Bacterial leaf streak (BLS), caused by Xanthomonas oryzae pv. oryzicola (Xoc), is a major bacterial disease in rice. Transcription activator-like effectors (TALEs) from Xanthomonas can induce host susceptibility (S) genes and facilitate infection. However, knowledge of the function of Xoc TALEs in promoting bacterial virulence is limited. In this study, we demonstrated the importance of Tal10a for the full virulence of Xoc. Through computational prediction and gene expression analysis, we identified the hexokinase gene OsHXK5 as a host target of Tal10a. Tal10a directly binds to the gene promoter region and activates the expression of OsHXK5. CRISPR/Cas9-mediated gene editing in the effector binding element (EBE) of OsHXK5 significantly increases rice resistance to Xoc, while OsHXK5 overexpression enhances the susceptibility of rice plants and impairs rice defense responses. Moreover, simultaneous editing of the promoters of OsSULTR3;6 and OsHXK5 confers robust resistance to Xoc in rice. Taken together, our findings highlight the role of Tal10a in targeting OsHXK5 to promote infection and suggest that OsHXK5 represents a potential target for engineering rice resistance to Xoc.
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Claims 7
Claim · model-written summary
Tal10a is important for full virulence of Xoc in rice.
Not reportedTaxon
Verbatim
e0“we demonstrated the importance of Tal10a for the full virulence of Xoc”
Claim · model-written summary
OsHXK5 was identified as a rice host target of Tal10a.
Not reportedTaxon
Verbatim
e0“we identified the hexokinase gene OsHXK5 as a host target of Tal10a”
Claim · model-written summary
Tal10a binds the OsHXK5 promoter and activates its expression.
Not reportedTaxon
Verbatim
e0“Tal10a directly binds to the gene promoter region and activates the expression of OsHXK5”
Claim · model-written summary
Editing the OsHXK5 effector binding element significantly increases rice resistance to Xoc.
- Taxon
- rice
Oryza sativae0 - Treatment
- CRISPR/Cas9-mediated gene editing in the effector binding element (EBE) of OsHXK5e0
- Interactor
- Xoce0pathogen
- Gene
- OsHXK5e0
- Outcome
- rice resistance to Xoce0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CRISPR/Cas9-mediated gene editing in the effector binding element (EBE) of OsHXK5 significantly increases rice resistance to Xoc”
Claim · model-written summary
OsHXK5 overexpression enhances rice susceptibility to Xoc.
- Taxon
- riceOryza sativae0
- Treatment
- OsHXK5 overexpressione0
- Gene
- OsHXK5e0
- Outcome
- susceptibility of rice plantse0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsHXK5 overexpression enhances the susceptibility of rice plants and impairs rice defense responses”
Claim · model-written summary
OsHXK5 overexpression impairs rice defense responses.
- Taxon
- riceOryza sativae0
- Treatment
- OsHXK5 overexpressione0
- Gene
- OsHXK5e0
- Outcome
- rice defense responsese0
- Direction
- impairse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsHXK5 overexpression enhances the susceptibility of rice plants and impairs rice defense responses”
Claim · model-written summary
Editing both OsSULTR3;6 and OsHXK5 promoters confers robust rice resistance to Xoc.
- Taxon
- riceOryza sativae0
- Treatment
- simultaneous editing of the promoters of OsSULTR3;6 and OsHXK5e0
- Interactor
- Xoce0pathogen
- Gene
- OsSULTR3;6 and OsHXK5e0
- Outcome
- resistance to Xoce0
- Direction
- conferse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“simultaneous editing of the promoters of OsSULTR3;6 and OsHXK5 confers robust resistance to Xoc in rice”
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