Abstract
Xanthomonas oryzae delivers transcription activator-like effectors (TALEs) into plant cells to facilitate infection. Following economic principles, the redundant TALEs are rarely identified in Xanthomonas. Previously, we identified the Tal2b, which activates the expression of the rice 2-oxoglutarate-dependent dioxygenase gene OsF3H03g to promote infection in the highly virulent strain of X. oryzae pv. oryzicola HGA4. Here, we reveal that another clustered TALE, Tal2c, also functioned as a virulence factor to target rice OsF3H04g, a homologue of OsF3H03g. Transferring Tal2c into RS105 induced expression of OsF3H04g to coincide with increased susceptibility in rice. Overexpressing OsF3H04g caused higher susceptibility and less salicylic acid (SA) production compared to wild-type plants. Moreover, CRISPR–Cas9 system-mediated editing of the effector-binding element in the promoters of OsF3H03g or OsF3H04g was found to specifically enhance resistance to Tal2b- or Tal2c-transferring strains, but had no effect on resistance to either RS105 or HGA4. Furthermore, transcriptome analysis revealed that several reported SA-related and defense-related genes commonly altered expression in OsF3H04g overexpression line compared with those identified in OsF3H03g overexpression line. Overall, our results reveal a functional redundancy mechanism of pathogenic virulence in Xoc in which tandem Tal2b and Tal2c specifically target homologues of host genes to interfere with rice immunity by reducing SA.
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Claims 10
Claim · model-written summary
Tal2c targeted rice OsF3H04g as a virulence factor.
- Taxon
- rice
Oryza sativae0 - Gene
- OsF3H04ge0
- Outcome
- virulence factore0
Not reportedInteractor
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tal2c, also functioned as a virulence factor to target rice OsF3H04g”
Claim · model-written summary
Transferring Tal2c into RS105 induced OsF3H04g expression.
- Taxon
- riceOryza sativae1
- Treatment
- Transferring Tal2c into RS105e0
- Gene
- OsF3H04ge0
- Outcome
- expression of OsF3H04ge0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transferring Tal2c into RS105 induced expression of OsF3H04g”
e1“rice OsF3H04g”
Claim · model-written summary
Tal2c transfer into RS105 increased rice susceptibility.
- Taxon
- riceOryza sativae0
- Treatment
- Transferring Tal2c into RS105e0
- Outcome
- susceptibilitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transferring Tal2c into RS105 induced expression of OsF3H04g to coincide with increased susceptibility in rice”
Claim · model-written summary
OsF3H04g overexpression increased susceptibility relative to wild-type plants.
- Taxon
- riceOryza sativae1
- Treatment
- Overexpressing OsF3H04ge0
- Gene
- OsF3H04ge0
- Outcome
- susceptibilitye0
- Direction
- highere0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpressing OsF3H04g caused higher susceptibility and less salicylic acid (SA) production compared to wild-type plants”
e1“rice OsF3H04g”
Claim · model-written summary
OsF3H04g overexpression reduced salicylic acid production relative to wild type.
- Taxon
- riceOryza sativae1
- Treatment
- Overexpressing OsF3H04ge0
- Gene
- OsF3H04ge0
- Outcome
- salicylic acid (SA) productione0
- Direction
- lesse0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpressing OsF3H04g caused higher susceptibility and less salicylic acid (SA) production compared to wild-type plants”
e1“rice OsF3H04g”
Claim · model-written summary
Editing the OsF3H03g promoter effector-binding element enhanced resistance to Tal2b-transferring strains.
- Taxon
- riceOryza sativae1
- Treatment
- editing of the effector-binding elemente0
- Gene
- OsF3H03ge0
- Outcome
- resistance to Tal2b- or Tal2c-transferring strainse0
- Direction
- enhancee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“editing of the effector-binding element in the promoters of OsF3H03g or OsF3H04g was found to specifically enhance resistance to Tal2b- or Tal2c-transferring strains”
e1“rice OsF3H04g”
Claim · model-written summary
Editing the OsF3H04g promoter effector-binding element enhanced resistance to Tal2c-transferring strains.
- Taxon
- riceOryza sativae1
- Treatment
- editing of the effector-binding elemente0
- Gene
- OsF3H04ge0
- Outcome
- resistance to Tal2b- or Tal2c-transferring strainse0
- Direction
- enhancee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“editing of the effector-binding element in the promoters of OsF3H03g or OsF3H04g was found to specifically enhance resistance to Tal2b- or Tal2c-transferring strains”
e1“rice OsF3H04g”
Claim · model-written summary
Promoter editing had no effect on resistance to RS105 or HGA4.
- Taxon
- riceOryza sativae1
- Outcome
- resistance to either RS105 or HGA4e0
- Direction
- no effecte0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“had no effect on resistance to either RS105 or HGA4”
e1“rice OsF3H04g”
Claim · model-written summary
SA-related and defense-related gene expression changed in OsF3H04g overexpression lines.
- Taxon
- riceOryza sativae1
- Gene
- SA-related and defense-related genese0
- Outcome
- expressione0
- Direction
- alterede0
- Comparator
- OsF3H03g overexpression linee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“several reported SA-related and defense-related genes commonly altered expression in OsF3H04g overexpression line compared with those identified in OsF3H03g overexpression line”
e1“rice OsF3H04g”
Claim · model-written summary
Tal2b and Tal2c redundantly target homologous rice genes to reduce SA and interfere with immunity.
- Taxon
- riceOryza sativae0
- Gene
- homologues of host genese0
- Outcome
- rice immunitye0
- Qualifier
- reducing SAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tandem Tal2b and Tal2c specifically target homologues of host genes to interfere with rice immunity by reducing SA”
The same questions in other papers
pathogen product · disease · Oryza sativa (rice)Positive and negative results
14 papers in the same direction as this paper · 8 in the opposite direction
- no effect
- positive
- negative
19 papers more in this group
See all 23 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.