Abstract
OsHKT1;1 in rice, belongs to the high-affinity K+ Transporter family, has been found to be involved in salt tolerance. OsHKT1;1 in japonica rice (Nipponbare) produces mRNA variants, but their functions remain elusive. In salt tolerant rice, Pokkali, eight OsHKT1;1 variants (V1-V8) were identified in addition to the full-length OsHKT1;1 (FL) cDNA. Absolute quantification by qPCR revealed that accumulation of OsHKT1;1-FL mRNA is minor in contrast to that of OsHKT1;1-V1, -V2, -V4, and -V7 mRNAs, all of which are predominant in shoots, while only V1 and V7 mRNAs are predominant in roots. Two electrode voltage clamp (TEVC) experiments using Xenopus laevis oocytes revealed that oocytes-expressing OsHKT1;1-FL from Pokkali exhibited inward-rectified currents in the presence of 96 mM Na+ as reported previously. Further TEVC analyses indicated that six of eight OsHKT1;1 variants elicited currents in a Na+ or a K+ bath solution. OsHKT1;1-V6 exhibited a similar inward rectification to the FL protein. Contrastingly, however, the rests mediated bidirectional currents in both Na+ and K+ bath solutions. These data suggest possibilities that novel mechanisms regulating the transport activity of OsHKT1;1 might exist, and that OsHKT1;1 variants might also carry out distinct physiological roles either independently or in combination with OsHKT1;1-FL.
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Claims 11
Claim · model-written summary
Eight OsHKT1;1 variants were identified in salt-tolerant Pokkali rice in addition to full-length cDNA.
- Taxon
- rice
Oryza sativae0 - Material
- Pokkalie0
- Gene
- OsHKT1;1e0
- Outcome
- eight OsHKT1;1 variants (V1-V8)e0
- Value
- eighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In salt tolerant rice, Pokkali, eight OsHKT1;1 variants (V1-V8) were identified in addition to the full-length OsHKT1;1 (FL) cDNA.”
Claim · model-written summary
Full-length OsHKT1;1 mRNA accumulation is minor relative to V1, V2, V4 and V7 mRNAs.
- Taxon
- RiceOryza sativat0
- Gene
- OsHKT1;1-FLe0
- Outcome
- accumulation of OsHKT1;1-FL mRNAe0
- Direction
- minore0
- Comparator
- OsHKT1;1-V1, -V2, -V4, and -V7 mRNAse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“accumulation of OsHKT1;1-FL mRNA is minor in contrast to that of OsHKT1;1-V1, -V2, -V4, and -V7 mRNAs”
t0“Rice”
Claim · model-written summary
V1 and V2 transcripts are predominant in shoots.
- Taxon
- RiceOryza sativat0
- Organ / stage
- shootse0
- Gene
- OsHKT1;1-V1, -V2e0
- Outcome
- predominant in shootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsHKT1;1-V1, -V2, -V4, and -V7 mRNAs, all of which are predominant in shoots”
t0“Rice”
Claim · model-written summary
V4 and V7 transcripts are predominant in shoots.
- Taxon
- RiceOryza sativat0
- Organ / stage
- shootse0
- Gene
- -V4, and -V7e0
- Outcome
- predominant in shootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsHKT1;1-V1, -V2, -V4, and -V7 mRNAs, all of which are predominant in shoots”
t0“Rice”
Claim · model-written summary
Only V1 and V7 transcripts are predominant in roots.
- Taxon
- RiceOryza sativat0
- Organ / stage
- rootse0
- Gene
- V1 and V7e0
- Outcome
- predominant in rootse0
- Qualifier
- onlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“only V1 and V7 mRNAs are predominant in roots”
t0“Rice”
Claim · model-written summary
Oocytes expressing Pokkali full-length OsHKT1;1 exhibit inward-rectified currents in 96 mM sodium.
- Taxon
- Xenopus laevis
Xenopus laevise0 - Material
- Pokkalie0
- Organ / stage
- oocytese0
- Treatment
- 96 mM Na+e0
- Gene
- OsHKT1;1-FLe0
- Outcome
- inward-rectified currentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two electrode voltage clamp (TEVC) experiments using Xenopus laevis oocytes revealed that oocytes-expressing OsHKT1;1-FL from Pokkali exhibited inward-rectified currents in the presence of 96 mM Na+ as reported previously.”
Claim · model-written summary
Six of eight OsHKT1;1 variants elicit currents in sodium or potassium bath solution.
- Taxon
- Xenopus laevisXenopus laevise1
- Treatment
- Na+ or a K+ bath solutione0
- Gene
- OsHKT1;1e0
- Outcome
- elicited currentse0
- Value
- six of eighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Further TEVC analyses indicated that six of eight OsHKT1;1 variants elicited currents in a Na+ or a K+ bath solution.”
e1“Xenopus laevis oocytes”
Claim · model-written summary
OsHKT1;1-V6 exhibits inward rectification similar to the full-length protein.
- Taxon
- Xenopus laevisXenopus laevise1
- Gene
- OsHKT1;1-V6e0
- Outcome
- inward rectificatione0
- Comparator
- FL proteine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsHKT1;1-V6 exhibited a similar inward rectification to the FL protein.”
e1“Xenopus laevis oocytes”
Claim · model-written summary
The remaining current-eliciting variants mediate bidirectional currents in both sodium and potassium solutions.
- Taxon
- Xenopus laevisXenopus laevise1
- Treatment
- Na+ and K+ bath solutionse0
- Outcome
- bidirectional currentse0
- Qualifier
- the restse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Contrastingly, however, the rests mediated bidirectional currents in both Na+ and K+ bath solutions.”
e1“Xenopus laevis oocytes”
Claim · model-written summary
The results suggest possible novel mechanisms regulating OsHKT1;1 transport activity.
- Taxon
- RiceOryza sativat0
- Gene
- OsHKT1;1e0
- Outcome
- transport activitye0
- Qualifier
- novel mechanismse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These data suggest possibilities that novel mechanisms regulating the transport activity of OsHKT1;1 might exist”
t0“Rice”
Claim · model-written summary
OsHKT1;1 variants may have distinct physiological roles independently or combined with full-length OsHKT1;1.
- Taxon
- RiceOryza sativat0
- Gene
- OsHKT1;1e0
- Outcome
- distinct physiological rolese0
- Qualifier
- either independently or in combination with OsHKT1;1-FLe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsHKT1;1 variants might also carry out distinct physiological roles either independently or in combination with OsHKT1;1-FL.”
t0“Rice”