Abstract
ABSTRACT Micropropagation of small fruits such as blackberry has been employed due to the need to obtain plants with high phytosanitary quality. Bioreactor technology has been used to improve efficiency in seedling production. Thus, the objective of this work was to evaluate the best culture medium volume and sucrose concentration for blackberry micropropagation in a temporary immersion bioreactor. In vitro blackberry shoots were segmented containing two buds and an internode (1.0 cm) and placed into MS medium supplemented with inositol (0.1 g L-1), BAP (1 mg L-1) and sucrose (10, 20, 30 or 40 g L-1) at different medium volumes (150, 175 and 200 mL). The total length, number of leaves, number of shoots, and number of hyperhydric shoots were evaluated 56 days after start of the project. For blackberry development and propagation in a bioreactor system, the best results were shown at a medium volume of 175 ml and a sucrose concentration of 20 g L-1.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 1
Claim · model-written summary
A 175 ml medium volume and 20 g L-1 sucrose gave the best blackberry development and propagation results.
- Treatment
- a medium volume of 175 ml and a sucrose concentration of 20 g L-1e0
- Outcome
- development and propagatione0
- Direction
- best resultse0
- Setting
- bioreactor systeme0
RubusNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“For blackberry development and propagation in a bioreactor system, the best results were shown at a medium volume of 175 ml and a sucrose concentration of 20 g L-1.”
The same questions in other papers
sucrose · growth · Rubus (bramble)Results agree
2 papers in the same direction as this paper
- positive
- positive
in vitro · growth · Rubus (bramble)Positive and negative results
12 papers in the same direction as this paper · 4 in the opposite direction
- no effect
- negative
- negative
13 papers more in this group
See all 17 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.