Abstract
The present experiment has been designed in order to see the effect of pruning on explants collection and it's in vitro performance on shoot initiation, shoot multiplication and root initiation. For this purpose, two species of guava namely, Psidium guajava L. and P. friedrichsthalianum (O. Berg) Nied. (Chinese guava) were selected. Healthy shoots were pruned at 50% and 75% level along with the unpruned shoots (control). Nodal segments of new growth were used as explants. Best MS media along with plant growth hormones obtained in our previous work were used for culturing explants. Explants collected from pruned shoots (50% and 75%) gave better shoot regeneration and shoot multiplication over the unpruned (control) shoots in case of P. guajava L cv. L-49. The highest numbers of shoot regeneration (64.2%) were recorded in explants obtained from 50% pruned shoots of P. friedrichsthalianum. Lowest shoot regeneration was recorded in explants collected from unpruned (39.3%) shoots in highest number of days (21.3). The highest number of root formation was obtained in explants obtained from 75% pruned shoots in both guava species over 50% pruned shoots with 66.3% in cv. L-49 and 64.3% in P. friedrichsthalianum. Hence, the explants when collected after pruning gave better results for in vitro shoot initiation, shoot multiplication and root formation over the explants collected from unpruned shoots (control).
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Claims 6
Claim · model-written summary
Explants from 50% and 75% pruned shoots gave better shoot regeneration and multiplication than unpruned controls in P. guajava cv. L-49.
- Taxon
- P. guajava L
Psidium guajavae0 - Material
- cv. L-49e0
- Organ / stage
- Nodal segmentse5
- Treatment
- pruned shoots (50% and 75%)e0
- Outcome
- shoot regeneration and shoot multiplicatione0
- Direction
- bettere0
- Comparator
- unpruned (control) shootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Explants collected from pruned shoots (50% and 75%) gave better shoot regeneration and shoot multiplication over the unpruned (control) shoots in case of P. guajava L cv. L-49”
e5“Nodal segments of new growth were used as explants”
Claim · model-written summary
In P. guajava cv. L-49, root formation was highest in explants from 75% pruned shoots, at 66.3%, above 50% pruned shoots.
- Taxon
- P. guajava LPsidium guajavae0
- Material
- cv. L-49e3
- Treatment
- 75% pruned shootse3
- Outcome
- root formatione3
- Direction
- higheste3
- Value
- 66.3%e3
- Comparator
- 50% pruned shootse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Explants collected from pruned shoots (50% and 75%) gave better shoot regeneration and shoot multiplication over the unpruned (control) shoots in case of P. guajava L cv. L-49”
e3“The highest number of root formation was obtained in explants obtained from 75% pruned shoots in both guava species over 50% pruned shoots with 66.3% in cv. L-49 and 64.3% in P. friedrichsthalianum”
Claim · model-written summary
In P. friedrichsthalianum, root formation was highest in explants from 75% pruned shoots, at 64.3%, above 50% pruned shoots.
- Taxon
- P. friedrichsthalianum
Psidium friedrichsthalianume3 - Treatment
- 75% pruned shootse3
- Outcome
- root formatione3
- Direction
- higheste3
- Value
- 64.3%e3
- Comparator
- 50% pruned shootse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“The highest number of root formation was obtained in explants obtained from 75% pruned shoots in both guava species over 50% pruned shoots with 66.3% in cv. L-49 and 64.3% in P. friedrichsthalianum”
Claim · model-written summary
In P. friedrichsthalianum, shoot regeneration was highest in explants from 50% pruned shoots, at 64.2%.
- Taxon
- P. friedrichsthalianumPsidium friedrichsthalianume1
- Treatment
- 50% pruned shootse1
- Outcome
- shoot regeneratione1
- Direction
- higheste1
- Value
- 64.2%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The highest numbers of shoot regeneration (64.2%) were recorded in explants obtained from 50% pruned shoots of P. friedrichsthalianum”
Claim · model-written summary
Shoot regeneration was lowest in explants from unpruned guava shoots, at 39.3%, and was reached in the highest number of days (21.3).
- Taxon
- guavaPsidium guajavae4
- Treatment
- unpruned (39.3%) shootse2
- Outcome
- shoot regeneratione2
- Direction
- Loweste2
- Value
- 39.3%e2
- Qualifier
- highest number of days (21.3)e2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Lowest shoot regeneration was recorded in explants collected from unpruned (39.3%) shoots in highest number of days (21.3)”
e4“two species of guava namely, Psidium guajava L. and P. friedrichsthalianum (O. Berg) Nied.”
Claim · model-written summary
Explants collected after pruning gave better root formation than explants from unpruned shoots.
- Taxon
- guavaPsidium guajavae4
- Treatment
- collected after pruninge6
- Outcome
- root formatione6
- Direction
- bettere6
- Comparator
- unpruned shoots (control)e6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“two species of guava namely, Psidium guajava L. and P. friedrichsthalianum (O. Berg) Nied.”
e6“the explants when collected after pruning gave better results for in vitro shoot initiation, shoot multiplication and root formation over the explants collected from unpruned shoots (control)”
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See all 41 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.