Abstract
To explore the accumulation characteristics of melatonin and underline mechanism in leaves of chinese cherry, the daily changes of melatonin content and the expression of synthetic genes were determined. The results showed that melatonin content increased gradually during the day and midnight, reaching the peak at 2:00 a.m, and then began to decrease, indicating that light regulate the synthesis of melatonin in plants. The expression profile of 5 melatonin synthesis genes was investigated by qRT-PCR, among them the mRNA transcript of PacSNAT , PacASMT and PacT5H2 increased and then decreased with time prolonging, in accordance with changes of melatonin content, indicating their key role in melatonin synthesis.
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Claims 3
Claim · model-written summary
Leaf melatonin increased through the day and midnight, peaked at 2:00 a.m., then decreased.
Verbatim
e0“melatonin content increased gradually during the day and midnight, reaching the peak at 2:00 a.m, and then began to decrease”
t0“Daily accumulation of melatonin and gene expression in chinese cherry”
Claim · model-written summary
PacSNAT, PacASMT and PacT5H2 transcripts first increased then decreased over time.
Verbatim
e0“the mRNA transcript of PacSNAT , PacASMT and PacT5H2 increased and then decreased with time prolonging”
t0“Daily accumulation of melatonin and gene expression in chinese cherry”
Claim · model-written summary
The transcript pattern aligned with changes in melatonin content.
Verbatim
e0“in accordance with changes of melatonin content, indicating their key role in melatonin synthesis.”
t0“Daily accumulation of melatonin and gene expression in chinese cherry”