Abstract
Regeneration protocol is pre-requisite for the development of transgenic plants. Maize (Zea mays L) crop is severely affected by fungal disease turcicum leaf blight. In the present study, standardizing the regeneration protocol for maize genotype was carried for the development of transgenic maize against turcicum leaf blight. The direct regeneration of maize involved nodal explants from the maize coleoptiles. Three Turcicum leaf blight susceptible maize genotypes (NAI179, NAI193, and HKI413) were selected for this study. Different combinations of growth regulators were used with MS media to induce shoots and roots. The best responding explants for the shooting was recorded in NAI179 on MS + 1.5mgL-1 BAP media. Fastest responding genotype was found to be HKI413 on MS + 0.5 mgL-1 BAP media. The maximum number of shoots were recorded in NAI179 on MS + mgL-1 BAP media and maximum shoot elongation was seen in NAI193 on MS + 2.5 mgL-1 BAP + 0.5 mgL-1 IBA. The best response for rooting was observed in NAI179 on MS + 0.5 mgL-1 BAP + 2.0 mgL-1 NAA media and fastest responding genotypes was found to be HKI413.
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Claims 6
Claim · model-written summary
NAI179 gave the best shooting response of maize explants on MS with 1.5 mg/L BAP.
- Material
- NAI179e2
- Organ / stage
- explantse2
- Treatment
- MS + 1.5mgL-1 BAP mediae2
- Outcome
- responding explants for the shootinge2
- Direction
- beste2
Zea maysNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Maize Genotypes”
e1“Three Turcicum leaf blight susceptible maize genotypes (NAI179, NAI193, and HKI413) were selected for this study”
e2“The best responding explants for the shooting was recorded in NAI179 on MS + 1.5mgL-1 BAP media”
Claim · model-written summary
HKI413 was the fastest responding maize genotype on MS with 0.5 mg/L BAP for shooting.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Maize Genotypes”
e3“Fastest responding genotype was found to be HKI413 on MS + 0.5 mgL-1 BAP media”
Claim · model-written summary
NAI179 gave the maximum number of shoots on MS with BAP.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Maize Genotypes”
e4“The maximum number of shoots were recorded in NAI179 on MS + mgL-1 BAP media”
Claim · model-written summary
NAI193 gave the maximum shoot elongation on MS with 2.5 mg/L BAP and 0.5 mg/L IBA.
- Material
- NAI193e5
- Treatment
- MS + 2.5 mgL-1 BAP + 0.5 mgL-1 IBAe5
- Outcome
- shoot elongatione5
- Direction
- maximume5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Maize Genotypes”
e5“maximum shoot elongation was seen in NAI193 on MS + 2.5 mgL-1 BAP + 0.5 mgL-1 IBA”
Claim · model-written summary
NAI179 gave the best rooting response on MS with 0.5 mg/L BAP and 2.0 mg/L NAA.
- Material
- NAI179e6
- Treatment
- MS + 0.5 mgL-1 BAP + 2.0 mgL-1 NAA mediae6
- Outcome
- rootinge6
- Direction
- best responsee6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Maize Genotypes”
e6“The best response for rooting was observed in NAI179 on MS + 0.5 mgL-1 BAP + 2.0 mgL-1 NAA media and fastest responding genotypes was found to be HKI413”
Claim · model-written summary
HKI413 was the fastest responding maize genotype in the rooting stage.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Maize Genotypes”
e6“The best response for rooting was observed in NAI179 on MS + 0.5 mgL-1 BAP + 2.0 mgL-1 NAA media and fastest responding genotypes was found to be HKI413”
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