Abstract
Wounding of plant tissues induces a massive change in gene expression. The phytohormone ethylene which is evolved in response to wounding of plant tissues has been implicated as one of the mediators of wound-induced changes in gene expression. Wounded unripe tomato pericarp tissue was treated with either 2,5 norbornadiene or silver thiosulfate to specifically inhibit the expression of ethylene-regulated mRNA species. Results show that wounding induces a dramatic shift in gene expression (over 50 mRNA species), but expression of less than 15% of these genes is affected by the ethylene action inhibitors. Wounded plant tissues accumulate many plant secondary products. DAHP synthase catalyzes the first reaction of the shikimate pathway that leads to the aromatic amino acids which are precursors of a number of secondary metabolites involved in plant defense and repair. Wounding Solanaceous tissues or treating parsley cells with fungal elicitor or light strongly induce DAHP synthase at the mRNA level. DAHP synthase mRNA accumulated coordinately with phenylalanine ammonia-lyase, potentially sharing regulatory factors which control primary and secondary metabolism. A tomato cDNA for DAHP synthase was cloned and sequenced. My data show that the tomato genome contains a single DAHP synthase gene which is induced by wounding but down regulated during fruit development and ripening, indicating environmental and developmental cues regulate the same gene. Transgenic potato plants were created to examine the expression of the DAHP synthase gene (shkA). The 5$\sp\prime$ region, flanking potato DAHP synthase coding sequences, was translationally fused to the reporter gene $\beta$-glucuronidase (GUS) and introduced into potato. Based on GUS activity, the shkA promoter conferred cell-specific and wound-inducible transgene expression similar to that of the endogenous DAHP synthase. However, the wound-induced accumulation of the endogenous DAHP synthase mRNA was inhibited by the expression of the introduced GUS transgene. This interaction suggests competition between endogenous and introduced homologous promoters for trans-acting transcription factors. Collectively, the data indicate that DAHP synthase is highly regulated and is an important control point for carbon flow through the shikimate pathway.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 14
Claim · model-written summary
Wounding shifted expression of over 50 mRNA species in tomato pericarp.
- Taxon
- tomato
Solanum lycopersicumt0 - Treatment
- woundinge0
- Outcome
- gene expressione0
- Value
- over 50 mRNA speciese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Results show that wounding induces a dramatic shift in gene expression (over 50 mRNA species), but expression of less than 15% of these genes is affected by the ethylene action inhibitors.”
t0“Wounded unripe tomato pericarp tissue”
Claim · model-written summary
Ethylene action inhibitors affected expression of less than 15% of wound-responsive genes.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- ethylene action inhibitorse0
- Outcome
- expressione0
- Value
- less than 15%e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Results show that wounding induces a dramatic shift in gene expression (over 50 mRNA species), but expression of less than 15% of these genes is affected by the ethylene action inhibitors.”
t0“Wounded unripe tomato pericarp tissue”
Claim · model-written summary
Wounding Solanaceous tissues strongly induced DAHP synthase mRNA.
Verbatim
e0“Wounding Solanaceous tissues or treating parsley cells with fungal elicitor or light strongly induce DAHP synthase at the mRNA level.”
Claim · model-written summary
Fungal elicitor strongly induced DAHP synthase mRNA in parsley cells.
- Taxon
- parsley
Petroselinum crispume0 - Treatment
- fungal elicitore0
- Gene
- DAHP synthasee0
- Outcome
- DAHP synthase at the mRNA levele0
- Direction
- strongly inducee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wounding Solanaceous tissues or treating parsley cells with fungal elicitor or light strongly induce DAHP synthase at the mRNA level.”
Claim · model-written summary
Light strongly induced DAHP synthase mRNA in parsley cells.
- Taxon
- parsleyPetroselinum crispume0
- Treatment
- lighte0
- Gene
- DAHP synthasee0
- Outcome
- DAHP synthase at the mRNA levele0
- Direction
- strongly inducee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wounding Solanaceous tissues or treating parsley cells with fungal elicitor or light strongly induce DAHP synthase at the mRNA level.”
Claim · model-written summary
DAHP synthase mRNA accumulated with phenylalanine ammonia-lyase.
- Taxon
- SolanaceaeSolanaceaet0
- Gene
- phenylalanine ammonia-lyasee0
- Outcome
- DAHP synthase mRNA accumulated coordinatelye0
Not reportedDirection
Verbatim
e0“DAHP synthase mRNA accumulated coordinately with phenylalanine ammonia-lyase, potentially sharing regulatory factors which control primary and secondary metabolism.”
t0“Solanaceae”
Claim · model-written summary
A tomato DAHP synthase cDNA was cloned and sequenced.
- Taxon
- tomatoSolanum lycopersicume0
- Gene
- DAHP synthasee0
- Outcome
- cDNAe0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A tomato cDNA for DAHP synthase was cloned and sequenced.”
Claim · model-written summary
The tomato genome contains a single DAHP synthase gene.
- Taxon
- tomatoSolanum lycopersicume0
- Gene
- DAHP synthasee0
- Outcome
- single DAHP synthase genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“My data show that the tomato genome contains a single DAHP synthase gene which is induced by wounding but down regulated during fruit development and ripening, indicating environmental and developmental cues regulate the same gene.”
Claim · model-written summary
Wounding induces the tomato DAHP synthase gene.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- woundinge0
- Gene
- DAHP synthasee0
- Outcome
- DAHP synthase genee0
- Direction
- inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“My data show that the tomato genome contains a single DAHP synthase gene which is induced by wounding but down regulated during fruit development and ripening, indicating environmental and developmental cues regulate the same gene.”
Claim · model-written summary
DAHP synthase is down regulated during tomato fruit development and ripening.
- Taxon
- tomatoSolanum lycopersicume0
- Organ / stage
- fruit development and ripeninge0
- Gene
- DAHP synthasee0
- Outcome
- DAHP synthase genee0
- Direction
- down regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“My data show that the tomato genome contains a single DAHP synthase gene which is induced by wounding but down regulated during fruit development and ripening, indicating environmental and developmental cues regulate the same gene.”
Claim · model-written summary
The potato shkA promoter conferred cell-specific transgene expression.
- Taxon
- potato
Solanum tuberosumt0 - Gene
- shkAe0
- Outcome
- cell-specifice0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Based on GUS activity, the shkA promoter conferred cell-specific and wound-inducible transgene expression similar to that of the endogenous DAHP synthase.”
t0“Transgenic potato plants were created”
Claim · model-written summary
The potato shkA promoter conferred wound-inducible transgene expression.
- Taxon
- potatoSolanum tuberosumt0
- Gene
- shkAe0
- Outcome
- wound-inducible transgene expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Based on GUS activity, the shkA promoter conferred cell-specific and wound-inducible transgene expression similar to that of the endogenous DAHP synthase.”
t0“Transgenic potato plants were created”
Claim · model-written summary
The GUS transgene inhibited wound-induced endogenous DAHP synthase mRNA accumulation.
- Taxon
- potatoSolanum tuberosumt0
- Treatment
- expression of the introduced GUS transgenee0
- Gene
- DAHP synthasee0
- Outcome
- wound-induced accumulation of the endogenous DAHP synthase mRNAe0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, the wound-induced accumulation of the endogenous DAHP synthase mRNA was inhibited by the expression of the introduced GUS transgene.”
t0“Transgenic potato plants were created”
Claim · model-written summary
The data indicate DAHP synthase is highly regulated and controls carbon flow through the shikimate pathway.
- Taxon
- SolanaceaeSolanaceaet0
- Gene
- DAHP synthasee0
- Outcome
- carbon flow through the shikimate pathwaye0
Verbatim
e0“Collectively, the data indicate that DAHP synthase is highly regulated and is an important control point for carbon flow through the shikimate pathway.”
t0“Solanaceae”
The same questions in other papers
transgenic · molecular: expression · Solanum tuberosum (potato)Positive and negative results
4 papers in the same direction as this paper · 4 in the opposite direction
- positive
- positive
- positive
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.