Abstract
We evaluated the effects of various plant growth regulator (PGR) combinations on adventitious shoot bud multiplication of date palm cv. Aziza Bouzid. In the first experiment, we assessed the effect of four PGR combinations that have already shown good results in other date palm cultivars: half-strength Murashige and Skoog medium (MS/2) supplemented with 0.5 mg/L 2-naphthoxyacetic acid (NOA) and 0.5 mg/L kinetin (KIN); MS/2 supplemented with 0.2 mg/L NOA, 0.2 mg/L indole-3-acetic acid (IAA), 0.4 mg/L 6-(dimethylallylamino)purine (2iP) and 0.4 mg/L KIN; MS/2 supplemented with 0.5 mg/L 1-naphthaleneacetic acid (NAA) and 0.5 mg/L KIN and finally MS/2 supplemented with 0.5 mg/L indole-3-butyric acid (IBA) and 0.6 mg/L 6-benzylaminopurine (BAP). Our results showed no significant difference among the four culture media in terms of adventitious shoot bud multiplication (26.7-28.4 adventitious buds per explant). However, the medium containing 0.5 mg/L NOA and 0.5 mg/L KIN showed significantly lower rate of precocious rooting and thus this auxin-cytokinin combination was chosen for the second experiment. In the second experiment, we evaluated the effects of various NOA and KIN concentrations on adventitious shoot bud multiplication: 0, 0.25, 0.5 and 1 mg/L of each PGR, either alone or in combination. It was found that the combination of 0.5 mg/L NOA and 0.5 mg/L KIN is still the most appropriate for adventitious shoot bud multiplication. Shoot elongation and rooting were achieved on PGR-free MS/2 medium and a high acclimatization rate of 70% was observed in the greenhouse.
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Claims 5
Claim · model-written summary
The four media did not differ significantly in date palm bud multiplication, producing 26.7–28.4 buds per explant.
- Taxon
- date palm
Phoenix dactyliferat0 - Material
- Aziza Bouzidt0
- Treatment
- four culture mediae0
- Outcome
- adventitious shoot bud multiplicatione0
- Direction
- no significant differencee0
- Value
- 26.7-28.4 adventitious buds per explante0
- Comparator
- four culture mediae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Our results showed no significant difference among the four culture media in terms of adventitious shoot bud multiplication (26.7-28.4 adventitious buds per explant).”
t0“date palm cv. Aziza Bouzid”
Claim · model-written summary
The medium with 0.5 mg/L NOA and 0.5 mg/L KIN significantly reduced precocious rooting relative to other media.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- Aziza Bouzidt0
- Treatment
- 0.5 mg/L NOA and 0.5 mg/L KINe0
- Outcome
- rate of precocious rootinge0
- Direction
- lowere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, the medium containing 0.5 mg/L NOA and 0.5 mg/L KIN showed significantly lower rate of precocious rooting and thus this auxin-cytokinin combination was chosen for the second experiment.”
t0“date palm cv. Aziza Bouzid”
Claim · model-written summary
The authors identified 0.5 mg/L NOA plus 0.5 mg/L KIN as the most appropriate combination for bud multiplication.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- Aziza Bouzidt0
- Outcome
- combination of 0.5 mg/L NOA and 0.5 mg/L KINe0
- Qualifier
- adventitious shoot bud multiplicatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It was found that the combination of 0.5 mg/L NOA and 0.5 mg/L KIN is still the most appropriate for adventitious shoot bud multiplication.”
t0“date palm cv. Aziza Bouzid”
Claim · model-written summary
Shoot elongation and rooting were achieved on regulator-free MS/2 medium.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- Aziza Bouzidt0
- Treatment
- PGR-free MS/2 mediume0
- Outcome
- Shoot elongation and rootinge0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Shoot elongation and rooting were achieved on PGR-free MS/2 medium”
t0“date palm cv. Aziza Bouzid”
Claim · model-written summary
Date palm acclimatization in the greenhouse reached 70%.
- Taxon
- date palmPhoenix dactyliferat0
- Material
- Aziza Bouzidt0
- Outcome
- acclimatization ratee0
- Direction
- highe0
- Value
- 70%e0
- Setting
- greenhousee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a high acclimatization rate of 70% was observed in the greenhouse.”
t0“date palm cv. Aziza Bouzid”
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