Paper · 2020 · Forests

Interaction of Biochar Type and Rhizobia Inoculation Increases the Growth and Biological Nitrogen Fixation of Robinia pseudoacacia Seedlings

Qiaoyu Sun; Yong Liu; Hongbin Liu; et al.

6
claims
13
verbatim quotes
23
citations
3
research areas
✓
Open access · cc-by

Abstract

Adding biochar to soil can change soil properties and subsequently affect plant growth, but this effect can vary because of different feedstocks and methods (e.g., pyrolysis or gasification) used to create the biochar. Growth and biological nitrogen fixation (BNF) of leguminous plants can be improved with rhizobia inoculation that fosters nodule development. Thus, this factorial greenhouse study examined the effects of two types of biochar (i.e., pyrolysis and gasification) added at a rate of 5% (v:v) to a peat-based growth substrate and rhizobia inoculation (yes or no) on Robinia pseudoacacia (black locust) seedlings supplied with 15NH415NO3. Seedling and nodule growth, nitrogen (N) content, and δ15N × 1000 were evaluated after 3 months. While addition of biochar without inoculation had no effect on seedling growth, inoculation with rhizobia increased seedling growth, BNF, and N status. Inoculated seedlings had reduced δ15N, indicating that N provided via fertilization was being diluted by N additions through BNF. Biochar type and inoculation interacted to affect seedling growth. Combining inoculation with either biochar type increased seedling leaf, stem, and total biomass, whereas gasifier biochar and inoculation improved all seedling growth variables and nodule biomass.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 6

Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Adding biochar without inoculation had no effect on seedling growth.

Taxon
Robinia pseudoacaciaRobinia pseudoacaciae1
Treatment
addition of biochar without inoculatione2
Outcome
seedling growthe2
Direction
no effecte2
Setting
greenhousee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Robinia pseudoacacia
Robinia pseudoacacia · TaxonAnnabell Hormann / korina.info · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“this factorial greenhouse study examined the effects of two types of biochar”

✓ located verbatim in the source · abstract, characters 367–443 · show in text ↑
e1

“Robinia pseudoacacia (black locust) seedlings”

✓ located verbatim in the source · abstract, characters 580–625 · show in text ↑
e2

“While addition of biochar without inoculation had no effect on seedling growth”

✓ located verbatim in the source · abstract, characters 749–827 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Rhizobia inoculation increased seedling growth, biological nitrogen fixation and nitrogen status.

Taxon
Robinia pseudoacaciaRobinia pseudoacaciae1
Treatment
inoculation with rhizobiae3
Interactor
rhizobiae3symbiont
Outcome
seedling growth, BNF, and N statuse3
Direction
increasede3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Robinia pseudoacacia
Robinia pseudoacacia · TaxonAnnabell Hormann / korina.info · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Robinia pseudoacacia (black locust) seedlings”

✓ located verbatim in the source · abstract, characters 580–625 · show in text ↑
e3

“inoculation with rhizobia increased seedling growth, BNF, and N status”

✓ located verbatim in the source · abstract, characters 829–899 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Inoculated seedlings had reduced δ15N values, indicating N from fertilization was diluted by N fixed through BNF.

Taxon
Robinia pseudoacaciaRobinia pseudoacaciae1
Treatment
Inoculated seedlingse4
Outcome
δ15Ne4
Direction
reducede4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Robinia pseudoacacia
Robinia pseudoacacia · TaxonAnnabell Hormann / korina.info · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Robinia pseudoacacia (black locust) seedlings”

✓ located verbatim in the source · abstract, characters 580–625 · show in text ↑
e4

“Inoculated seedlings had reduced δ15N”

✓ located verbatim in the source · abstract, characters 901–938 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Biochar type and inoculation interacted to affect seedling growth.

Taxon
Robinia pseudoacaciaRobinia pseudoacaciae1
Treatment
Biochar type and inoculatione5
Outcome
seedling growthe5

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Robinia pseudoacacia
Robinia pseudoacacia · TaxonAnnabell Hormann / korina.info · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Robinia pseudoacacia (black locust) seedlings”

✓ located verbatim in the source · abstract, characters 580–625 · show in text ↑
e5

“Biochar type and inoculation interacted to affect seedling growth”

✓ located verbatim in the source · abstract, characters 1031–1096 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Combining inoculation with either biochar type increased seedling leaf, stem and total biomass.

Taxon
Robinia pseudoacaciaRobinia pseudoacaciae1
Treatment
Combining inoculation with either biochar typee6
Outcome
seedling leaf, stem, and total biomasse6
Direction
increasede6

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Robinia pseudoacacia
Robinia pseudoacacia · TaxonAnnabell Hormann / korina.info · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Robinia pseudoacacia (black locust) seedlings”

✓ located verbatim in the source · abstract, characters 580–625 · show in text ↑
e6

“Combining inoculation with either biochar type increased seedling leaf, stem, and total biomass”

✓ located verbatim in the source · abstract, characters 1098–1193 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Gasifier biochar combined with inoculation improved all seedling growth variables and nodule biomass.

Taxon
Robinia pseudoacaciaRobinia pseudoacaciae1
Treatment
gasifier biochar and inoculatione7
Outcome
all seedling growth variables and nodule biomasse7
Direction
improvede7

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Robinia pseudoacacia
Robinia pseudoacacia · TaxonAnnabell Hormann / korina.info · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Robinia pseudoacacia (black locust) seedlings”

✓ located verbatim in the source · abstract, characters 580–625 · show in text ↑
e7

“gasifier biochar and inoculation improved all seedling growth variables and nodule biomass”

✓ located verbatim in the source · abstract, characters 1203–1293 · show in text ↑

inoculation · symbiosis · Robinia pseudoacacia (black locust)Positive and negative results

3 papers: 2 positive, 1 negative or no effect

1 paper in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
See all 3 papers →

biochar · growth · Robinia pseudoacacia (black locust)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

inoculation · growth · Robinia pseudoacacia (black locust)Results agree

11 papers: 11 positive, 0 negative or no effect

10 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

7 papers more in this group

See all 11 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.