Abstract
Abstract Germin-like proteins (GLPs) are a diverse and ubiquitous family of plant glycoproteins belonging to the cupin super family; they play considerable roles in plant responses against various abiotic and biotic stresses. Here, we provide evidence that GLP2 protein from cotton ( Gossypium hirsutum ) functions in plant defense responses against Verticillium dahliae , Fusarium oxysporum and oxidative stress. Purified recombinant GhGLP2 exhibits superoxide dismutase (SOD) activity and inhibits spore germination of pathogens. Virus-induced silencing of GhGLP2 in cotton results in increased susceptibility to pathogens, plants exhibited severe wilt on leaves, enhanced vascular browning and suppressed callose deposition. Transgenic Arabidopsis ( Arabidopsis thaliana ) plants overexpressing GhGLP2 showed significant resistance to V. dahliae and F. oxysporum , with reduced mycelia growth, increased callose deposition and cell wall lignification at infection sites on leaves. The enhanced tolerance of GhGLP2 -transgenic Arabidopsis to oxidative stress was investigated by methyl viologen and ammonium persulfate treatments, along with increased H 2 O 2 production. Further, the expression of several defense-related genes ( PDF1.2 , LOX2 , and VSP1 ) or oxidative stress-related genes ( RbohD , RbohF ) was triggered by GhGLP2 . Thus, our results confirmed the involvement of GhGLP2 in plant defense response against Verticillium and Fusarium wilt pathogens and stress conditions.
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Claims 14
Claim · model-written summary
Virus-induced silencing of GhGLP2 in cotton increased susceptibility to pathogens.
- Taxon
- cotton
Gossypium hirsutume1 - Treatment
- Virus-induced silencing of GhGLP2e1
- Interactor
- pathogense1pathogen
- Gene
- GhGLP2e1
- Outcome
- susceptibility to pathogense1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Virus-induced silencing of GhGLP2 in cotton results in increased susceptibility to pathogens”
Claim · model-written summary
Virus-induced silencing of GhGLP2 in cotton plants exhibited severe wilt on leaves.
- Taxon
- cottonGossypium hirsutume1
- Treatment
- Virus-induced silencing of GhGLP2e1
- Gene
- GhGLP2e1
- Outcome
- severe wilt on leavese1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Virus-induced silencing of GhGLP2 in cotton results in increased susceptibility to pathogens, plants exhibited severe wilt on leaves, enhanced vascular browning and suppressed callose deposition”
Claim · model-written summary
Virus-induced silencing of GhGLP2 in cotton enhanced vascular browning.
- Taxon
- cottonGossypium hirsutume1
- Treatment
- Virus-induced silencing of GhGLP2e1
- Gene
- GhGLP2e1
- Outcome
- vascular browninge1
- Direction
- enhancede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Virus-induced silencing of GhGLP2 in cotton results in increased susceptibility to pathogens, plants exhibited severe wilt on leaves, enhanced vascular browning and suppressed callose deposition”
Claim · model-written summary
Virus-induced silencing of GhGLP2 in cotton suppressed callose deposition.
- Taxon
- cottonGossypium hirsutume1
- Treatment
- Virus-induced silencing of GhGLP2e1
- Gene
- GhGLP2e1
- Outcome
- callose depositione1
- Direction
- suppressede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Virus-induced silencing of GhGLP2 in cotton results in increased susceptibility to pathogens, plants exhibited severe wilt on leaves, enhanced vascular browning and suppressed callose deposition”
Claim · model-written summary
Recombinant GhGLP2 from cotton exhibits superoxide dismutase (SOD) activity.
- Taxon
- cottonGossypium hirsutumt0
- Gene
- GhGLP2e1
- Outcome
- superoxide dismutase (SOD) activitye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton GhGLP2”
e1“Purified recombinant GhGLP2 exhibits superoxide dismutase (SOD) activity”
Claim · model-written summary
Purified recombinant GhGLP2 inhibits spore germination of pathogens.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- Purified recombinant GhGLP2e1
- Interactor
- pathogense1pathogen
- Gene
- GhGLP2e1
- Outcome
- spore germination of pathogense1
- Direction
- inhibitse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton GhGLP2”
e1“Purified recombinant GhGLP2 exhibits superoxide dismutase (SOD) activity and inhibits spore germination of pathogens”
Claim · model-written summary
Transgenic Arabidopsis overexpressing GhGLP2 showed significant resistance to V. dahliae.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae1 - Treatment
- overexpressing GhGLP2e1
- Interactor
- V. dahliae
Verticillium dahliaee1pathogen - Gene
- GhGLP2e1
- Outcome
- resistancee1
- Direction
- increaseinferrede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Transgenic Arabidopsis ( Arabidopsis thaliana ) plants overexpressing GhGLP2 showed significant resistance to V. dahliae and F. oxysporum , with reduced mycelia growth, increased callose deposition and cell wall lignification at infection sites on leaves”
Claim · model-written summary
Transgenic Arabidopsis overexpressing GhGLP2 showed significant resistance to F. oxysporum.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- overexpressing GhGLP2e1
- Interactor
- F. oxysporum
_(Fusarium_oxysporum_f.sp._batatas)_1234227-PPT-Clemson_University.jpg?width=60)
Fusarium oxysporume1pathogen - Gene
- GhGLP2e1
- Outcome
- resistancee1
- Direction
- increaseinferrede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_(Fusarium_oxysporum_f.sp._batatas)_1234227-PPT-Clemson_University.jpg?width=500)
Verbatim
e1“Transgenic Arabidopsis ( Arabidopsis thaliana ) plants overexpressing GhGLP2 showed significant resistance to V. dahliae and F. oxysporum , with reduced mycelia growth, increased callose deposition and cell wall lignification at infection sites on leaves”
Claim · model-written summary
Transgenic Arabidopsis overexpressing GhGLP2 showed reduced mycelia growth of the pathogens.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- overexpressing GhGLP2e1
- Gene
- GhGLP2e1
- Outcome
- mycelia growthe1
- Direction
- reducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Transgenic Arabidopsis ( Arabidopsis thaliana ) plants overexpressing GhGLP2 showed significant resistance to V. dahliae and F. oxysporum , with reduced mycelia growth, increased callose deposition and cell wall lignification at infection sites on leaves”
Claim · model-written summary
Transgenic Arabidopsis overexpressing GhGLP2 showed increased callose deposition and cell wall lignification at infection sites.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- overexpressing GhGLP2e1
- Gene
- GhGLP2e1
- Outcome
- callose deposition and cell wall lignificatione1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Transgenic Arabidopsis ( Arabidopsis thaliana ) plants overexpressing GhGLP2 showed significant resistance to V. dahliae and F. oxysporum , with reduced mycelia growth, increased callose deposition and cell wall lignification at infection sites on leaves”
Claim · model-written summary
GhGLP2-transgenic Arabidopsis showed enhanced tolerance to oxidative stress.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- methyl viologen and ammonium persulfate treatmentse1
- Gene
- GhGLP2e1
- Outcome
- tolerancee1
- Direction
- enhancede1
- Qualifier
- to oxidative stresse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The enhanced tolerance of GhGLP2 -transgenic Arabidopsis to oxidative stress was investigated by methyl viologen and ammonium persulfate treatments, along with increased H 2 O 2 production”
Claim · model-written summary
GhGLP2-transgenic Arabidopsis showed increased H 2 O 2 production.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- GhGLP2 -transgenice1
- Gene
- GhGLP2e1
- Outcome
- H 2 O 2 productione1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The enhanced tolerance of GhGLP2 -transgenic Arabidopsis to oxidative stress was investigated by methyl viologen and ammonium persulfate treatments, along with increased H 2 O 2 production”
Claim · model-written summary
GhGLP2 triggered expression of defence-related genes PDF1.2, LOX2 and VSP1.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- GhGLP2e1
- Gene
- PDF1.2 , LOX2 , and VSP1e1
- Outcome
- expression of several defense-related genese1
- Direction
- increaseinferrede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton GhGLP2”
e1“Further, the expression of several defense-related genes ( PDF1.2 , LOX2 , and VSP1 ) or oxidative stress-related genes ( RbohD , RbohF ) was triggered by GhGLP2”
Claim · model-written summary
GhGLP2 triggered expression of oxidative stress-related genes RbohD and RbohF.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- GhGLP2e1
- Gene
- RbohD , RbohFe1
- Outcome
- oxidative stress-related genese1
- Direction
- increaseinferrede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton GhGLP2”
e1“Further, the expression of several defense-related genes ( PDF1.2 , LOX2 , and VSP1 ) or oxidative stress-related genes ( RbohD , RbohF ) was triggered by GhGLP2”
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