Abstract
Abstract The unfolded protein response (UPR) and the heat shock response (HSR) are two evolutionarily conserved systems that protect plants from heat stress. The UPR and HSR occur in different cellular compartments and both responses are elicited by misfolded proteins that accumulate under adverse environmental conditions such as heat stress. While the UPR and HSR appear to operate independently, we have found a link between them in maize (Zea mays) involving the production of the BASIC LEUCINE ZIPPER60 (bZIP60) transcription factor, a pivotal response of the UPR to heat stress. Surprisingly, a mutant (bzip60-2) knocking down bZIP60 expression blunted the HSR at elevated temperatures and prevented the normal upregulation of a group of heat shock protein genes in response to elevated temperature. The expression of a key HEAT SHOCK FACTOR TRANSCRIPTION FACTOR13 (HSFTF13, a HEAT SHOCK FACTOR A6B [HSFA6B] family member) was compromised in bzip60-2, and the HSFTF13 promoter was shown to be a target of bZIP60 in maize protoplasts. In addition, the upregulation by heat of genes involved in chlorophyll catabolism and chloroplast protein turnover were subdued in bzip60-2, and these genes were also found to be targets of bZIP60. Thus, the UPR, an endoplasmic-reticulum–associated response, quite unexpectedly contributes to the nuclear/cytoplasmic HSR in maize.
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Claims 7
Claim · model-written summary
The bzip60-2 mutant, with bZIP60 knocked down, blunted the heat shock response at elevated temperatures.
- Material
- bzip60-2e1
- Treatment
- knocking down bZIP60 expressione1
- Gene
- bZIP60e1
- Outcome
- HSRe1
- Direction
- bluntede1
- Qualifier
- at elevated temperaturese1
Zea maysNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in maize (Zea mays) involving the production of the BASIC LEUCINE ZIPPER60 (bZIP60) transcription factor”
e1“a mutant (bzip60-2) knocking down bZIP60 expression blunted the HSR at elevated temperatures and prevented the normal upregulation of a group of heat shock protein genes in response to elevated temperature”
Claim · model-written summary
The bzip60-2 mutant prevented the normal upregulation of heat shock protein genes at elevated temperature.
- Material
- bzip60-2e1
- Treatment
- elevated temperaturee1
- Outcome
- upregulation of a group of heat shock protein genese1
- Direction
- preventede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in maize (Zea mays) involving the production of the BASIC LEUCINE ZIPPER60 (bZIP60) transcription factor”
e1“a mutant (bzip60-2) knocking down bZIP60 expression blunted the HSR at elevated temperatures and prevented the normal upregulation of a group of heat shock protein genes in response to elevated temperature”
Claim · model-written summary
HSFTF13 expression was compromised in the bzip60-2 mutant.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in maize (Zea mays) involving the production of the BASIC LEUCINE ZIPPER60 (bZIP60) transcription factor”
e2“The expression of a key HEAT SHOCK FACTOR TRANSCRIPTION FACTOR13 (HSFTF13, a HEAT SHOCK FACTOR A6B [HSFA6B] family member) was compromised in bzip60-2”
Claim · model-written summary
The HSFTF13 promoter is a direct target of bZIP60 in maize protoplasts.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in maize (Zea mays) involving the production of the BASIC LEUCINE ZIPPER60 (bZIP60) transcription factor”
e3“the HSFTF13 promoter was shown to be a target of bZIP60 in maize protoplasts”
Claim · model-written summary
Heat upregulation of chlorophyll catabolism and chloroplast protein turnover genes was subdued in bzip60-2.
- Material
- bzip60-2e4
- Treatment
- heate4
- Gene
- genes involved in chlorophyll catabolism and chloroplast protein turnovere4
- Outcome
- the upregulation by heat of genese4
- Direction
- subduede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in maize (Zea mays) involving the production of the BASIC LEUCINE ZIPPER60 (bZIP60) transcription factor”
e4“the upregulation by heat of genes involved in chlorophyll catabolism and chloroplast protein turnover were subdued in bzip60-2”
Claim · model-written summary
The chlorophyll catabolism and chloroplast protein turnover genes are targets of bZIP60.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in maize (Zea mays) involving the production of the BASIC LEUCINE ZIPPER60 (bZIP60) transcription factor”
e5“these genes were also found to be targets of bZIP60”
Claim · model-written summary
The UPR contributes to the nuclear/cytoplasmic heat shock response in maize.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“the UPR, an endoplasmic-reticulum–associated response, quite unexpectedly contributes to the nuclear/cytoplasmic HSR in maize”
The same questions in other papers
heat · molecular: expression · Zea mays (maize)Positive and negative results
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