Abstract
Continuous effort to improve in vitro culture and genetic transformation system are needed to increase the rice yield. In this study, three widely cultivated rice varieties (Naveen, IR64 and Swarna) were tested on six different media for callus induction. Similarly, three different regeneration media were examined for their efficiency in shoot induction. The MS medium supplemented with 3mgL-1 BAP, 1.0mgL-1 kinetin and 0.5 mgL-1 NAA showed better regeneration efficiency in IR64 and Swarna. In case of Naveen variety, maximum green shoot regeneration was achieved on MS medium supplemented with 2.0 mgL-1 Kinetin and 0.5 mgL-1 NAA. All the regenerated plantlets developed good root system in MS medium with 1.0mgL-1 NAA. Concentration of acetosyringone (AS) were optimized for Agrobacterium mediated transformation. 100μM AS in co-cultivation media was found to be the optimum concentration for Naveen, whereas 150μM AS was recorded as suitable for IR64 and Swarna. The results show that the modified media significantly improved the callus induction, regeneration and transformation efficiency of all three cultivars tested.
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Claims 6
Claim · model-written summary
The BAP, kinetin and NAA supplemented MS medium gave better regeneration efficiency in IR64 and Swarna.
- Taxon
- rice
Oryza sativat0 - Material
- IR64 and Swarnae0
- Treatment
- MS medium supplemented with 3mgL-1 BAP, 1.0mgL-1 kinetin and 0.5 mgL-1 NAAe0
- Outcome
- regeneration efficiencye0
- Direction
- bettere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The MS medium supplemented with 3mgL-1 BAP, 1.0mgL-1 kinetin and 0.5 mgL-1 NAA showed better regeneration efficiency in IR64 and Swarna.”
t0“rice varieties”
Claim · model-written summary
Naveen achieved maximum green shoot regeneration on MS medium with kinetin and NAA.
- Taxon
- riceOryza sativat0
- Material
- Naveene0
- Treatment
- MS medium supplemented with 2.0 mgL-1 Kinetin and 0.5 mgL-1 NAAe0
- Outcome
- green shoot regeneratione0
- Direction
- maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In case of Naveen variety, maximum green shoot regeneration was achieved on MS medium supplemented with 2.0 mgL-1 Kinetin and 0.5 mgL-1 NAA.”
t0“rice varieties”
Claim · model-written summary
All regenerated rice plantlets developed a good root system on MS medium with NAA.
- Taxon
- riceOryza sativat0
- Organ / stage
- root systeme0
- Treatment
- MS medium with 1.0mgL-1 NAAe0
- Outcome
- developed good root systeme0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All the regenerated plantlets developed good root system in MS medium with 1.0mgL-1 NAA.”
t0“rice varieties”
Claim · model-written summary
For Naveen, 100μM acetosyringone in co-cultivation medium was optimal for transformation.
- Taxon
- riceOryza sativat0
- Material
- Naveene0
- Treatment
- 100μM AS in co-cultivation mediae0
- Outcome
- optimum concentratione0
- Direction
- optimume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“100μM AS in co-cultivation media was found to be the optimum concentration for Naveen, whereas 150μM AS was recorded as suitable for IR64 and Swarna.”
t0“rice varieties”
Claim · model-written summary
For IR64 and Swarna, 150μM acetosyringone was suitable for transformation.
- Taxon
- riceOryza sativat0
- Material
- IR64 and Swarnae0
- Treatment
- 150μM ASe0
- Outcome
- suitablee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“100μM AS in co-cultivation media was found to be the optimum concentration for Naveen, whereas 150μM AS was recorded as suitable for IR64 and Swarna.”
t0“rice varieties”
Claim · model-written summary
Modified media significantly improved callus induction, regeneration and transformation efficiency of the three rice cultivars.
- Taxon
- riceOryza sativat0
- Treatment
- modified mediae0
- Outcome
- callus induction, regeneration and transformation efficiencye0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results show that the modified media significantly improved the callus induction, regeneration and transformation efficiency of all three cultivars tested.”
t0“rice varieties”
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