Abstract
Growth of rice callus tissue is discouraged when methionine is excluded from CMAA medium. While determining the methionine elimination effect, the amino acid interrelationships among methionine, lysine, threonine and isoleucine in the nutrition of the callus tissue were found. Poor growth, found in cultures on methionine deficient media was seen only when the media contained both threonine and lysine, simultaneously. The substitution of homoserine for methionine was also observed. Determination of free amino acid composition in tissues revealed that free methionine was barely detectable in tissues grown with sufficient amounts of threonine and lysine. When the concentration of either threonine or lysine was reduced, the free methionine content of the tissue increased. When the methionine deficient medium was supplemented with homoserine, the free methionine in the tissue increased, although the tissue retained a considerable amount of free threonine and lysine. Cultivation of tissue on an isoleucine deficient medium resulted in a significant decrease in free threonine content. These experimental results suggest that the biosynthetic pathway to methionine is cooperatively inhibited by threonine and lysine, and that threonine decomposition is inhibited by its end product isoleucine.
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Claims 9
Claim · model-written summary
Removing methionine from CMAA medium discouraged rice callus growth.
- Taxon
- rice
Oryza sativae0 - Organ / stage
- callus tissuee0
- Treatment
- methionine is excluded from CMAA mediume0
- Outcome
- Growth of rice callus tissuee0
- Direction
- discouragede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Growth of rice callus tissue is discouraged when methionine is excluded from CMAA medium”
Claim · model-written summary
Methionine deficient media caused poor growth only with both threonine and lysine.
- Taxon
- riceOryza sativat0
- Treatment
- methionine deficient mediae0
- Outcome
- Poor growthe0
- Direction
- Poore0
- Qualifier
- both threonine and lysine, simultaneouslye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice callus tissue”
e0“Poor growth, found in cultures on methionine deficient media was seen only when the media contained both threonine and lysine, simultaneously”
Claim · model-written summary
Homoserine could substitute for methionine.
- Taxon
- riceOryza sativat0
- Outcome
- substitution of homoserine for methioninee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice callus tissue”
e0“The substitution of homoserine for methionine was also observed”
Claim · model-written summary
Free methionine was barely detectable with sufficient threonine and lysine.
- Taxon
- riceOryza sativat0
- Outcome
- free methioninee0
- Direction
- barely detectablee0
- Qualifier
- sufficient amounts of threonine and lysinee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice callus tissue”
e0“free methionine was barely detectable in tissues grown with sufficient amounts of threonine and lysine”
Claim · model-written summary
Reducing either threonine or lysine increased free methionine in tissue.
- Taxon
- riceOryza sativat0
- Treatment
- concentration of either threonine or lysine was reducede0
- Outcome
- free methionine content of the tissuee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice callus tissue”
e0“When the concentration of either threonine or lysine was reduced, the free methionine content of the tissue increased”
Claim · model-written summary
Homoserine supplementation raised free methionine despite substantial free threonine and lysine.
- Taxon
- riceOryza sativat0
- Treatment
- supplemented with homoserinee0
- Outcome
- free methionine in the tissuee0
- Direction
- increasede0
- Qualifier
- considerable amount of free threonine and lysinee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice callus tissue”
e0“When the methionine deficient medium was supplemented with homoserine, the free methionine in the tissue increased, although the tissue retained a considerable amount of free threonine and lysine”
Claim · model-written summary
Isoleucine deficiency significantly decreased free threonine.
- Taxon
- riceOryza sativat0
- Treatment
- isoleucine deficient mediume0
- Outcome
- free threonine contente0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice callus tissue”
e0“Cultivation of tissue on an isoleucine deficient medium resulted in a significant decrease in free threonine content”
Claim · model-written summary
Threonine and lysine cooperatively inhibit methionine biosynthesis.
- Taxon
- riceOryza sativat0
- Compound
- threonine and lysinee0
- Outcome
- biosynthetic pathway to methioninee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice callus tissue”
e0“biosynthetic pathway to methionine is cooperatively inhibited by threonine and lysine”
Claim · model-written summary
Isoleucine inhibits threonine decomposition.
- Taxon
- riceOryza sativat0
- Compound
- isoleucinee0
- Outcome
- threonine decompositione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice callus tissue”
e0“threonine decomposition is inhibited by its end product isoleucine”
The same questions in other papers
lysine · molecular: metabolites · Oryza sativa (rice)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- negative
- positive
methionine · growth · Oryza sativa (rice)Results agree
3 papers in the same direction as this paper
- negative
- negative
- negative
in vitro · molecular: metabolites · Oryza sativa (rice)Positive and negative results
6 papers in the same direction as this paper · 6 in the opposite direction
- no effect
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9 papers more in this group
See all 13 papers →in vitro · growth · Oryza sativa (rice)Positive and negative results
24 papers in the same direction as this paper · 140 in the opposite direction
- positive
- positive
- positive
161 papers more in this group
See all 165 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.