Abstract
Experiments were performed with 10-day-old seedlings of wheat (Triticum aestivum L.) grown on sand saturated with Knop’s solution at a temperature of 22/18°С by natural light (16 h, 200 µmole quanta/(m2 s). Phytochrome was activated by means of an array of light diodes emitting light in the red spectral region (RL, λ = 656 nm, λ1/2 = 26 nm), inactivation was induced with an array of light diodes emitting far red light (FRL, λ = 737 nm, λ1/2 = 30 nm). At the end of the night period (8 h), activity of the NAD-dependent PGA-dehydrogenase complex (3-phosphoglycerate: ATP phosphotransferase and D-glyceraldehyde-3-phosphate:NAD+ oxidoreductase) in the direction of 3-PGA → 1,3-BPG → G3P was 6.0–7.0 µmoles of oxidized NADH/(min g fr wt). At the highest dose of RL radiation (20 min, 17.6 kJ/m2) incident on intact plant leaves, enzyme activity decreased by 35–40%. Longer exposure to RL (30 and 40 min) did not cause additional changes in enzyme activity. When exposure to RL was followed by FRL (20 min, 3.00 kJ/m2), the inactivating effect of RL on the enzyme complex was negated. It was shown that 5-min-long exposure to RL already caused a decrease in the rate of NADH oxidation by 10–15%, and enzyme activity decreased linearly when the dose rose. When plants exposed to RL were accommodated in the dark for up to 120 min, the half-life of the RL-inactivated state of the NAD-PGA dehydrogenase complex was 30–45 min. Thus, a dynamic regulation of the energy-transforming phase of glycolysis in wheat leaves mediated by the phytochrome system was shown and investigated. Such a photocompetent low-energy system can operate as a regulator of total oxidative processes (glycolysis + the Krebs cycle) in the course of daily transition from dark to light and back.
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Claims 8
Claim · model-written summary
Night end PGA dehydrogenase complex activity was 6.0–7.0 µmoles of oxidized NADH/(min g fr wt).
- Taxon
- Wheat
Triticum aestivumt0 - Gene
- NAD-dependent PGA-dehydrogenase complexe0
- Outcome
- activity of the NAD-dependent PGA-dehydrogenase complexe0
- Value
- 6.0–7.0 µmoles of oxidized NADH/(min g fr wt)e0
- Qualifier
- At the end of the night period (8 h)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At the end of the night period (8 h), activity of the NAD-dependent PGA-dehydrogenase complex (3-phosphoglycerate: ATP phosphotransferase and D-glyceraldehyde-3-phosphate:NAD+ oxidoreductase) in the direction of 3-PGA → 1,3-BPG → G3P was 6.0–7.0 µmoles of oxidized NADH/(min g fr wt).”
t0“Wheat Leaves”
Claim · model-written summary
The highest red light dose decreased wheat leaf enzyme activity by 35–40%.
- Taxon
- WheatTriticum aestivumt0
- Organ / stage
- intact plant leavese0
- Treatment
- RL radiation (20 min, 17.6 kJ/m2)e0
- Outcome
- enzyme activitye0
- Direction
- decreasede0
- Value
- 35–40%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At the highest dose of RL radiation (20 min, 17.6 kJ/m2) incident on intact plant leaves, enzyme activity decreased by 35–40%.”
t0“Wheat Leaves”
Claim · model-written summary
Longer red light exposure caused no further change in enzyme activity.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- RL (30 and 40 min)e0
- Outcome
- enzyme activitye0
- Direction
- did not cause additional changese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Longer exposure to RL (30 and 40 min) did not cause additional changes in enzyme activity.”
t0“Wheat Leaves”
Claim · model-written summary
Far red light after red light negated red light inactivation of the enzyme complex.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- FRL (20 min, 3.00 kJ/m2)e0
- Outcome
- inactivating effect of RL on the enzyme complexe0
- Direction
- negatede0
- Comparator
- RLe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When exposure to RL was followed by FRL (20 min, 3.00 kJ/m2), the inactivating effect of RL on the enzyme complex was negated.”
t0“Wheat Leaves”
Claim · model-written summary
Five minutes of red light decreased NADH oxidation rate by 10–15%.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- 5-min-long exposure to RLe0
- Outcome
- rate of NADH oxidatione0
- Direction
- decreasee0
- Value
- 10–15%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“5-min-long exposure to RL already caused a decrease in the rate of NADH oxidation by 10–15%”
t0“Wheat Leaves”
Claim · model-written summary
Enzyme activity fell linearly as red light dose rose.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- dosee0
- Outcome
- enzyme activitye0
- Direction
- decreased linearlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“enzyme activity decreased linearly when the dose rose”
t0“Wheat Leaves”
Claim · model-written summary
The red light inactivated enzyme state had a 30–45 min half life in darkness.
- Taxon
- WheatTriticum aestivumt0
- Outcome
- half-life of the RL-inactivated state of the NAD-PGA dehydrogenase complexe0
- Value
- 30–45 mine0
- Qualifier
- in the dark for up to 120 mine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When plants exposed to RL were accommodated in the dark for up to 120 min, the half-life of the RL-inactivated state of the NAD-PGA dehydrogenase complex was 30–45 min.”
t0“Wheat Leaves”
Claim · model-written summary
The authors infer phytochrome mediated regulation of the energy transforming phase of glycolysis in wheat leaves.
- Taxon
- wheatTriticum aestivume0
- Organ / stage
- leavese0
- Outcome
- dynamic regulation of the energy-transforming phase of glycolysise0
- Qualifier
- mediated by the phytochrome systeme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a dynamic regulation of the energy-transforming phase of glycolysis in wheat leaves mediated by the phytochrome system was shown and investigated.”
The same questions in other papers
radiation · soil: biology · Triticum aestivum (wheat)Positive and negative results
2 in the opposite direction
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.