Abstract
BRI1-EMS-SUPPRESSOR 1 (BES1) transcription factor is closely associated with the brassinosteroid (BR) signaling pathway and plays an important role in plant growth and development. SLB3 is a member of BES1 transcription factor family and its expression was previously shown to increase significantly in tomato seedlings under drought stress. In the present study, we used virus-induced gene silencing (VIGS) technology to downregulate SLB3 expression to reveal the function of the SLB3 gene under drought stress further. The downregulated expression of SLB3 weakened the drought tolerance of the plants appeared earlier wilting and higher accumulation of H2O2 and O2−·, decreased superoxide dismutase (SOD) activity, and increased proline (PRO) and malondialdehyde (MDA) contents and peroxidase (POD) activity. Quantitative real-time PCR (qRT-PCR) analysis of BR-related genes revealed that the expression of SlCPD, SlDWARF and BIN2-related genes was significantly upregulated in SLB3-silenced seedlings under drought stress, but that the expression of TCH4-related genes was downregulated. These results showed that silencing the SLB3 gene reduced the drought resistance of tomato plants and had an impact on the BR signaling transduction which may be probably responsible for the variation in drought resistance of the tomato plants.
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Claims 9
Claim · model-written summary
SLB3 downregulation weakened drought tolerance and caused earlier wilting.
- Taxon
- tomato
Solanum lycopersicumt0 - Treatment
- downregulated expression of SLB3e0
- Gene
- SLB3e0
- Outcome
- drought tolerance of the plantse0
- Direction
- weakenede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The downregulated expression of SLB3 weakened the drought tolerance of the plants appeared earlier wilting”
t0“tomato”
Claim · model-written summary
SLB3 silencing increased H2O2 accumulation.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- Silencingt1
- Gene
- SLB3t1
- Outcome
- accumulation of H2O2e0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“higher accumulation of H2O2 and O2−·”
t0“tomato”
t1“Silencing the SLB3 transcription factor gene decreases drought stress tolerance in tomato”
Claim · model-written summary
SLB3 silencing increased O2−· accumulation.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“higher accumulation of H2O2 and O2−·”
t0“tomato”
t1“Silencing the SLB3 transcription factor gene decreases drought stress tolerance in tomato”
Claim · model-written summary
SLB3 silencing decreased SOD activity.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- Silencingt1
- Gene
- SLB3t1
- Outcome
- superoxide dismutase (SOD) activitye0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“decreased superoxide dismutase (SOD) activity”
t0“tomato”
t1“Silencing the SLB3 transcription factor gene decreases drought stress tolerance in tomato”
Claim · model-written summary
SLB3 silencing increased proline content.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- Silencingt1
- Gene
- SLB3t1
- Outcome
- proline (PRO)e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased proline (PRO) and malondialdehyde (MDA) contents and peroxidase (POD) activity”
t0“tomato”
t1“Silencing the SLB3 transcription factor gene decreases drought stress tolerance in tomato”
Claim · model-written summary
SLB3 silencing increased malondialdehyde content.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- Silencingt1
- Gene
- SLB3t1
- Outcome
- malondialdehyde (MDA) contentse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased proline (PRO) and malondialdehyde (MDA) contents and peroxidase (POD) activity”
t0“tomato”
t1“Silencing the SLB3 transcription factor gene decreases drought stress tolerance in tomato”
Claim · model-written summary
SLB3 silencing increased POD activity.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- Silencingt1
- Gene
- SLB3t1
- Outcome
- peroxidase (POD) activitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased proline (PRO) and malondialdehyde (MDA) contents and peroxidase (POD) activity”
t0“tomato”
t1“Silencing the SLB3 transcription factor gene decreases drought stress tolerance in tomato”
Claim · model-written summary
SLB3 silencing upregulated SlCPD, SlDWARF and BIN2-related genes under drought.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- SLB3-silencede0
- Gene
- SlCPD, SlDWARF and BIN2-related genese0
- Outcome
- expressione0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of SlCPD, SlDWARF and BIN2-related genes was significantly upregulated in SLB3-silenced seedlings under drought stress”
t0“tomato”
Claim · model-written summary
SLB3 silencing downregulated TCH4-related genes under drought.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- Silencingt1
- Gene
- TCH4-related genese0
- Outcome
- expressione0
- Direction
- downregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of TCH4-related genes was downregulated”
t0“tomato”
t1“Silencing the SLB3 transcription factor gene decreases drought stress tolerance in tomato”
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