Abstract
Adverse environmental factors like salt stress, drought, and extreme temperatures, cause damage to plant growth, development, and crop yield. GRAS transcription factors (TFs) have numerous functions in biological processes. Some studies have reported that the GRAS protein family plays significant functions in plant growth and development under abiotic stresses. In this study, we demonstrated the functional characterization of a tomato SlGRAS10 gene under abiotic stresses such as salt stress and drought. Down-regulation of SlGRAS10 by RNA interference (RNAi) produced dwarf plants with smaller leaves, internode lengths, and enhanced flavonoid accumulation. We studied the effects of abiotic stresses on RNAi and wild-type (WT) plants. Moreover, SlGRAS10-RNAi plants were more tolerant to abiotic stresses (salt, drought, and Abscisic acid) than the WT plants. Down-regulation of SlGRAS10 significantly enhanced the expressions of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) to reduce the effects of reactive oxygen species (ROS) such as O2− and H2O2. Malondialdehyde (MDA) and proline contents were remarkably high in SlGRAS10-RNAi plants. Furthermore, the expression levels of chlorophyll biosynthesis, flavonoid biosynthesis, and stress-related genes were also enhanced under abiotic stress conditions. Collectively, our conclusions emphasized the significant function of SlGRAS10 as a stress tolerate transcription factor in a certain variety of abiotic stress tolerance by enhancing osmotic potential, flavonoid biosynthesis, and ROS scavenging system in the tomato plant.
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Claims 13
Claim · model-written summary
SlGRAS10 RNA interference produced dwarf tomato plants with smaller leaves and internodes.
- Taxon
- tomato
Solanum lycopersicumt0 - Treatment
- RNA interference (RNAi)e0
- Gene
- SlGRAS10e0
- Outcome
- dwarf plants with smaller leaves, internode lengthse0
- Direction
- smallere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Down-regulation of SlGRAS10 by RNA interference (RNAi) produced dwarf plants with smaller leaves, internode lengths”
t0“Tomato”
Claim · model-written summary
SlGRAS10 RNA interference enhanced flavonoid accumulation.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- RNA interference (RNAi)e0
- Gene
- SlGRAS10e0
- Outcome
- flavonoid accumulatione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Down-regulation of SlGRAS10 by RNA interference (RNAi) produced dwarf plants with smaller leaves, internode lengths, and enhanced flavonoid accumulation.”
t0“Tomato”
Claim · model-written summary
SlGRAS10 RNAi plants tolerated salt stress better than wild type plants.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- SlGRAS10-RNAi plantse0
- Treatment
- salte0
- Gene
- SlGRAS10e0
- Outcome
- tolerante0
- Direction
- moree0
- Comparator
- WT plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlGRAS10-RNAi plants were more tolerant to abiotic stresses (salt, drought, and Abscisic acid) than the WT plants.”
t0“Tomato”
Claim · model-written summary
SlGRAS10 RNAi plants tolerated drought better than wild type plants.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- SlGRAS10-RNAi plantse0
- Treatment
- droughte0
- Gene
- SlGRAS10e0
- Outcome
- tolerante0
- Direction
- moree0
- Comparator
- WT plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlGRAS10-RNAi plants were more tolerant to abiotic stresses (salt, drought, and Abscisic acid) than the WT plants.”
t0“Tomato”
Claim · model-written summary
SlGRAS10 RNAi plants tolerated abscisic acid better than wild type plants.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- SlGRAS10-RNAi plantse0
- Treatment
- Abscisic acide0
- Gene
- SlGRAS10e0
- Outcome
- tolerante0
- Direction
- moree0
- Comparator
- WT plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlGRAS10-RNAi plants were more tolerant to abiotic stresses (salt, drought, and Abscisic acid) than the WT plants.”
t0“Tomato”
Claim · model-written summary
SlGRAS10 down-regulation increased CAT expression.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- Down-regulation of SlGRAS10e0
- Gene
- catalase (CAT)e0
- Outcome
- expressions of catalase (CAT)e0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Down-regulation of SlGRAS10 significantly enhanced the expressions of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD)”
t0“Tomato”
Claim · model-written summary
SlGRAS10 down-regulation increased POD expression.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- Down-regulation of SlGRAS10e0
- Gene
- peroxidase (POD)e0
- Outcome
- expressions of catalase (CAT), peroxidase (POD)e0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Down-regulation of SlGRAS10 significantly enhanced the expressions of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD)”
t0“Tomato”
Claim · model-written summary
SlGRAS10 down-regulation increased SOD expression.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- Down-regulation of SlGRAS10e0
- Gene
- superoxide dismutase (SOD)e0
- Outcome
- expressions of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD)e0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Down-regulation of SlGRAS10 significantly enhanced the expressions of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD)”
t0“Tomato”
Claim · model-written summary
Malondialdehyde content was high in SlGRAS10 RNAi plants.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- SlGRAS10-RNAi plantse0
- Outcome
- Malondialdehyde (MDA)e0
- Direction
- highe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Malondialdehyde (MDA) and proline contents were remarkably high in SlGRAS10-RNAi plants.”
t0“Tomato”
Claim · model-written summary
Proline content was high in SlGRAS10 RNAi plants.
- Taxon
- tomatoSolanum lycopersicumt0
- Material
- SlGRAS10-RNAi plantse0
- Outcome
- proline contentse0
- Direction
- highe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Malondialdehyde (MDA) and proline contents were remarkably high in SlGRAS10-RNAi plants.”
t0“Tomato”
Claim · model-written summary
Chlorophyll biosynthesis gene expression increased under abiotic stress.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- abiotic stress conditionse0
- Outcome
- expression levels of chlorophyll biosynthesise0
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression levels of chlorophyll biosynthesis, flavonoid biosynthesis, and stress-related genes were also enhanced under abiotic stress conditions.”
t0“Tomato”
Claim · model-written summary
Flavonoid biosynthesis gene expression increased under abiotic stress.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- abiotic stress conditionse0
- Outcome
- flavonoid biosynthesise0
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression levels of chlorophyll biosynthesis, flavonoid biosynthesis, and stress-related genes were also enhanced under abiotic stress conditions.”
t0“Tomato”
Claim · model-written summary
Stress-related gene expression increased under abiotic stress.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- abiotic stress conditionse0
- Outcome
- stress-related genese0
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression levels of chlorophyll biosynthesis, flavonoid biosynthesis, and stress-related genes were also enhanced under abiotic stress conditions.”
t0“Tomato”
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