Abstract
The production of anthocyanin is regulated by light and corresponding photoreceptors. In this study, we found that exposure to blue light and overexpression of CRY1a are associated with increased accumulation of anthocyanin in tomato (Solanum lycopersicum L.). These responses are the result of changes in mRNA and the protein levels of SlHY5, which is a transcription factor. In vitro and in vivo experiments using electrophoretic mobility shift assay and ChIP-qPCR assays revealed that SlHY5 could directly recognize and bind to the G-box and ACGT-containing element in the promoters of anthocyanin biosynthesis genes, such as chalcone synthase 1, chalcone synthase 2, and dihydroflavonol 4-reductase. Silencing of SlHY5 in OE-CRY1a lines decreased the accumulation of anthocyanin. The findings presented here not only deepened our understanding of how light controls anthocyanin biosynthesis and associated photoprotection in tomato leaves, but also allowed us to explore potential targets for improving pigment production.
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Claims 4
Claim · model-written summary
Blue light exposure and CRY1a overexpression are associated with increased anthocyanin accumulation in tomato.
- Taxon
- tomato
Solanum lycopersicume1 - Treatment
- exposure to blue light and overexpression of CRY1ae1
- Outcome
- accumulation of anthocyanine1
- Direction
- increasede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In this study, we found that exposure to blue light and overexpression of CRY1a are associated with increased accumulation of anthocyanin in tomato (Solanum lycopersicum L.)”
Claim · model-written summary
The anthocyanin responses are the result of changes in mRNA and protein levels of SlHY5 in tomato.
- Taxon
- tomatoSolanum lycopersicume1
- Gene
- SlHY5e2
- Outcome
- responsese2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In this study, we found that exposure to blue light and overexpression of CRY1a are associated with increased accumulation of anthocyanin in tomato (Solanum lycopersicum L.)”
e2“These responses are the result of changes in mRNA and the protein levels of SlHY5, which is a transcription factor”
Claim · model-written summary
SlHY5 binds the G-box and ACGT-containing element in the promoters of anthocyanin biosynthesis genes in tomato.
- Taxon
- tomatoSolanum lycopersicume1
- Gene
- SlHY5e3
- Outcome
- bind to the G-box and ACGT-containing elemente3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In this study, we found that exposure to blue light and overexpression of CRY1a are associated with increased accumulation of anthocyanin in tomato (Solanum lycopersicum L.)”
e3“In vitro and in vivo experiments using electrophoretic mobility shift assay and ChIP-qPCR assays revealed that SlHY5 could directly recognize and bind to the G-box and ACGT-containing element in the promoters of anthocyanin biosynthesis genes, such as chalcone synthase 1, chalcone synthase 2, and dihydroflavonol 4-reductase”
Claim · model-written summary
Silencing SlHY5 in OE-CRY1a tomato lines decreased anthocyanin accumulation.
- Taxon
- tomatoSolanum lycopersicume1
- Material
- OE-CRY1a linese4
- Treatment
- Silencing of SlHY5 in OE-CRY1a linese4
- Gene
- SlHY5e4
- Outcome
- accumulation of anthocyanine4
- Direction
- decreasede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“In this study, we found that exposure to blue light and overexpression of CRY1a are associated with increased accumulation of anthocyanin in tomato (Solanum lycopersicum L.)”
e4“Silencing of SlHY5 in OE-CRY1a lines decreased the accumulation of anthocyanin”
The same questions in other papers
loss of function · quality: phytochemicals · Solanum lycopersicum (tomato)Positive and negative results
9 papers in the same direction as this paper · 6 in the opposite direction
- negative
- positive
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12 papers more in this group
See all 16 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.