Abstract
Hirschmanniella oryzae is one of the most devastating nematodes on rice, leading to substantial yield losses. Effector proteins aid the nematode during the infection process by subduing plant defence responses. In this research we characterized two potential H. oryzae effector proteins, chorismate mutase (HoCM) and isochorismatase (HoICM), and investigated their enzymatic activity and their role in plant immunity. Both HoCM and HoICM proved to be enzymatically active in complementation tests in mutant Escherichia coli strains. Infection success by the migratory nematode H. oryzae was significantly higher in transgenic rice lines constitutively expressing HoCM or HoICM. Expression of HoCM, but not HoICM, increased rice susceptibility against the sedentary nematode Meloidogyne graminicola also. Transcriptome and metabolome analyses indicated reductions in secondary metabolites in the transgenic rice plants expressing the potential nematode effectors. The results presented here demonstrate that both HoCM and HoICM suppress the host immune system and that this may be accomplished by lowering secondary metabolite levels in the plant.
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Claims 5
Claim · model-written summary
Infection success by H. oryzae was higher in transgenic rice lines expressing HoCM or HoICM.
- Taxon
- rice
Oryza sativae1 - Treatment
- transgenic rice lines constitutively expressing HoCM or HoICMe1
- Interactor
- H. oryzaeHirschmanniella oryzaee1pest
- Gene
- HoCMe1
- Outcome
- Infection successe1
- Direction
- highere1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Infection success by the migratory nematode H. oryzae was significantly higher in transgenic rice lines constitutively expressing HoCM or HoICM”
Claim · model-written summary
Expression of HoCM increased rice susceptibility to Meloidogyne graminicola.
- Taxon
- riceOryza sativae2
- Treatment
- Expression of HoCMe2
- Interactor
- Meloidogyne graminicolaMeloidogyne graminicolae2pest
- Gene
- HoCMe2
- Outcome
- susceptibilitye2
- Direction
- increasede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Expression of HoCM, but not HoICM, increased rice susceptibility against the sedentary nematode Meloidogyne graminicola also”
Claim · model-written summary
Transgenic rice expressing the nematode effectors had reduced secondary metabolites.
- Taxon
- riceOryza sativae3
- Treatment
- transgenic rice plants expressing the potential nematode effectorse3
- Outcome
- secondary metabolitese3
- Direction
- reductionse3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Transcriptome and metabolome analyses indicated reductions in secondary metabolites in the transgenic rice plants expressing the potential nematode effectors”
Claim · model-written summary
HoCM and HoICM suppress the rice immune system, possibly by lowering secondary metabolite levels.
- Taxon
- riceOryza sativae0
- Gene
- HoCM and HoICMe4
- Outcome
- suppress the host immune systeme4
- Qualifier
- lowering secondary metabolite levelse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hirschmanniella oryzae is one of the most devastating nematodes on rice”
e4“both HoCM and HoICM suppress the host immune system and that this may be accomplished by lowering secondary metabolite levels in the plant”
Claim · model-written summary
HoCM and HoICM are both enzymatically active in E. coli complementation tests.
- Taxon
- riceOryza sativae0
- Gene
- HoCM and HoICMe5
- Outcome
- enzymatically activee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hirschmanniella oryzae is one of the most devastating nematodes on rice”
e5“Both HoCM and HoICM proved to be enzymatically active in complementation tests in mutant Escherichia coli strains”
The same questions in other papers
transgenic · Meloidogyne graminicola · Oryza sativa (rice)Positive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.