Abstract
Ectopic expression of FOREVER YOUNG FLOWER (FYF) delays floral senescence and abscission in transgenic Arabidopsis. To analyze the FYF function in Phalaenopsis orchids, two FYF-like genes (PaFYF1/2) were identified. PaFYF1/2 were highly expressed in young Phalaenopsis flowers, and their expression decreased significantly afterward until flower senescence. This pattern was strongly correlated with the process of flower senescence and revealed that PaFYF1/2 function to suppress senescence/abscission during early flower development. Interestingly, in flowers, PaFYF1 was consistently expressed less in petals than in lips/sepals, whereas PaFYF2 was expressed relatively evenly in all flower organs. This difference suggests a regulatory modification of the functions of PaFYF1 and PaFYF2 during Phalaenopsis flower evolution. Delayed flower senescence and abscission, which were unaffected by ethylene treatment, were observed in 35S::PaFYF1/2 and 35S::PaFYF1/2 + SRDX transgenic Arabidopsis plants due to the downregulation of the ethylene signaling and abscission-associated genes EDF1-4, IDA and BOP1/2. These results suggest a possible repressor role for Phalaenopsis PaFYF1/2 in controlling floral senescence/abscission by suppressing ethylene signaling and abscission-associated genes. To further validate the function of PaFYF1/2, PaFYF1/2-VIGS (virus-induced gene silencing) Phalaenopsis were generated and analyzed. Promotion of senescence and abscission was observed in PaFYF1/2-VIGS Phalaenopsis flowers by the upregulation of PeEDF1/2, PeSAG39 and PeBOP1/2 expression, the early occurrence of greening according to their increased chlorophyll content and the reduction in water content in flower organs. Our results support that PaFYF1/2 function as transcriptional repressors to prohibit flower senescence and abscission in Phalaenopsis.
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Claims 10
Claim · model-written summary
Two FYF-like genes were identified in Phalaenopsis.
Verbatim
e0“To analyze the FYF function in Phalaenopsis orchids, two FYF-like genes (PaFYF1/2) were identified.”
Claim · model-written summary
PaFYF1/2 expression was high in young flowers and declined toward senescence.
- Taxon
- Phalaenopsis
_(47706143821).jpg?width=60)
Phalaenopsise0 - Organ / stage
- young Phalaenopsis flowerse0
- Gene
- PaFYF1/2e0
- Outcome
- expressione0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_(47706143821).jpg?width=500)
Verbatim
e0“PaFYF1/2 were highly expressed in young Phalaenopsis flowers, and their expression decreased significantly afterward until flower senescence.”
Claim · model-written summary
PaFYF1 expression was lower in petals than lips and sepals.
- Taxon
- PhalaenopsisPhalaenopsist0
- Organ / stage
- petalse0
- Gene
- PaFYF1e0
- Outcome
- expressede0
- Direction
- lesse0
- Comparator
- lips/sepalse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PaFYF1 was consistently expressed less in petals than in lips/sepals”
t0“Phalaenopsis Orchids”
Claim · model-written summary
PaFYF2 was expressed relatively evenly across flower organs.
- Taxon
- PhalaenopsisPhalaenopsist0
- Organ / stage
- flower organse0
- Gene
- PaFYF2e0
- Outcome
- expressed relatively evenlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PaFYF2 was expressed relatively evenly in all flower organs.”
t0“Phalaenopsis Orchids”
Claim · model-written summary
PaFYF transgenes delayed Arabidopsis flower senescence and abscission even under ethylene treatment.
- Taxon
- Arabidopsis
Arabidopsise0 - Material
- 35S::PaFYF1/2 and 35S::PaFYF1/2 + SRDX transgenic Arabidopsis plantse0
- Treatment
- ethylene treatmente0
- Outcome
- flower senescence and abscissione0
- Direction
- Delayede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Delayed flower senescence and abscission, which were unaffected by ethylene treatment, were observed in 35S::PaFYF1/2 and 35S::PaFYF1/2 + SRDX transgenic Arabidopsis plants”
Claim · model-written summary
PaFYF transgenes downregulated ethylene signaling and abscission genes.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- EDF1-4, IDA and BOP1/2e0
- Outcome
- downregulation of the ethylene signaling and abscission-associated genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Delayed flower senescence and abscission, which were unaffected by ethylene treatment, were observed in 35S::PaFYF1/2 and 35S::PaFYF1/2 + SRDX transgenic Arabidopsis plants due to the downregulation of the ethylene signaling and abscission-associated genes EDF1-4, IDA and BOP1/2.”
Claim · model-written summary
PaFYF1/2 silencing promoted senescence and abscission in Phalaenopsis flowers.
- Taxon
- PhalaenopsisPhalaenopsise0
- Treatment
- PaFYF1/2-VIGSe0
- Gene
- PaFYF1/2e0
- Outcome
- senescence and abscissione0
- Direction
- Promotione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Promotion of senescence and abscission was observed in PaFYF1/2-VIGS Phalaenopsis flowers”
Claim · model-written summary
Silenced Phalaenopsis flowers had increased expression of senescence-related genes.
- Taxon
- PhalaenopsisPhalaenopsist0
- Gene
- PeEDF1/2, PeSAG39 and PeBOP1/2e0
- Outcome
- expressione0
- Direction
- upregulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“by the upregulation of PeEDF1/2, PeSAG39 and PeBOP1/2 expression”
t0“Phalaenopsis Orchids”
Claim · model-written summary
Silenced flowers greened early with increased chlorophyll.
- Taxon
- PhalaenopsisPhalaenopsist0
- Outcome
- chlorophyll contente0
- Direction
- increasede0
Not reportedOrgan / stage
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the early occurrence of greening according to their increased chlorophyll content”
t0“Phalaenopsis Orchids”
Claim · model-written summary
Silenced flower organs had reduced water content.
- Taxon
- PhalaenopsisPhalaenopsist0
- Organ / stage
- flower organse0
- Outcome
- water contente0
- Direction
- reductione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the reduction in water content in flower organs.”
t0“Phalaenopsis Orchids”
The same questions in other papers
ethene · quality: postharvest · ArabidopsisPositive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.