Abstract
Abstract Ex vitro rooting and acclimatization of two cultivars ‘Wojtek’ and ‘Zojka’ of blue honeysuckle ( Lonicera caerulea var. kamtschatica Sevast.) were studied. To the ex vitro conditions were transferred rooted and unrooted shoots. The post-effect of auxin type and concentration as well as microcutting and soil substrate types were tested. The genetic stability of the plantlets in relation to the mother plants by using amplified fragment length polymorphism (AFLP) and inter simple sequence repeat (ISSR) markers has been also determined. It has been found that in vitro rooted cuttings of both cultivars showed a higher survival rate (max. 88%) and better growth and development when they were rooted on a medium containing a low auxin level (1.0 mg·dm −3 ). The results of the second experiment showed successful ex vitro rooting of blue honeysuckle shoots without auxin treatment. Higher ex vitro rooting and survival rate in the greenhouse have been observed for ‘Wojtek’ (max. 96%) than ‘Zojka’ (max. 88%). Better growth and development of shoots and roots were observed on peat alone or a mixture of peat and perlite as compared to a mixture of peat and sand. The micropropagated plantlets appeared similar to mother plants. Molecular analysis confirmed a high level of genetic stability of blue honeysuckle after 2 years of in vitro propagation. However, among the cultivars studied, ‘Wojtek’ showed slightly higher genetic stability than ‘Zojka’ (99.5% and 97.7%, respectively). For ‘Zojka’ plants, the degree of variation was comparable for AFLP and ISSR markers. For ‘Wojtek’, no polymorphism was detected using the ISSR analysis in contrast to the AFLP analysis.
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Claims 10
Claim · model-written summary
In vitro rooted cuttings of both blue honeysuckle cultivars had a higher survival rate (max. 88%) when rooted on a medium with a low auxin level.
- Taxon
- Lonicera caerulea var. kamtschatica
Lonicera caerulea var. kamtschaticat0 - Treatment
- a medium containing a low auxin levele1
- Outcome
- survival ratee0
- Direction
- highere0
- Value
- max. 88%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Lonicera caerulea var. kamtschatica”
e0“in vitro rooted cuttings of both cultivars showed a higher survival rate (max. 88%)”
e1“rooted on a medium containing a low auxin level”
Claim · model-written summary
In vitro rooted cuttings of both cultivars showed better growth and development when rooted on a medium with a low auxin level.
- Taxon
- Lonicera caerulea var. kamtschaticaLonicera caerulea var. kamtschaticat0
- Treatment
- a medium containing a low auxin levele0
- Outcome
- growth and developmente0
- Direction
- bettere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Lonicera caerulea var. kamtschatica”
e0“better growth and development when they were rooted on a medium containing a low auxin level”
Claim · model-written summary
Ex vitro rooting of blue honeysuckle shoots was successful without auxin treatment.
- Taxon
- blue honeysuckle
Lonicera caeruleae0 - Outcome
- successful ex vitro rootinge0
- Qualifier
- without auxin treatmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“successful ex vitro rooting of blue honeysuckle shoots without auxin treatment”
Claim · model-written summary
Ex vitro rooting and survival rate in the greenhouse were higher for cultivar Wojtek (max. 96%) than for Zojka (max. 88%).
- Taxon
- Lonicera caerulea var. kamtschaticaLonicera caerulea var. kamtschaticat0
- Material
- Wojteke0
- Outcome
- ex vitro rooting and survival ratee0
- Direction
- Highere0
- Value
- max. 96%e0
- Qualifier
- max. 88%e0
- Comparator
- Zojkae0
- Setting
- greenhousee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Lonicera caerulea var. kamtschatica”
e0“Higher ex vitro rooting and survival rate in the greenhouse have been observed for ‘Wojtek’ (max. 96%) than ‘Zojka’ (max. 88%)”
Claim · model-written summary
Shoots and roots grew and developed better on peat alone or a peat-perlite mixture than on a peat-sand mixture.
- Taxon
- Lonicera caerulea var. kamtschaticaLonicera caerulea var. kamtschaticat0
- Treatment
- peat alone or a mixture of peat and perlitee0
- Outcome
- growth and development of shoots and rootse0
- Direction
- Bettere0
- Comparator
- a mixture of peat and sande0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Lonicera caerulea var. kamtschatica”
e0“Better growth and development of shoots and roots were observed on peat alone or a mixture of peat and perlite as compared to a mixture of peat and sand”
Claim · model-written summary
The micropropagated plantlets appeared similar to the mother plants.
- Taxon
- Lonicera caerulea var. kamtschaticaLonicera caerulea var. kamtschaticat0
- Outcome
- similar to mother plantse0
- Direction
- similare0
- Comparator
- mother plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Lonicera caerulea var. kamtschatica”
e0“The micropropagated plantlets appeared similar to mother plants”
Claim · model-written summary
Molecular analysis confirmed a high level of genetic stability of blue honeysuckle after 2 years of in vitro propagation.
- Taxon
- blue honeysuckleLonicera caeruleae0
- Outcome
- high level of genetic stabilitye0
- Qualifier
- after 2 years of in vitro propagatione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Molecular analysis confirmed a high level of genetic stability of blue honeysuckle after 2 years of in vitro propagation”
Claim · model-written summary
Cultivar Wojtek showed slightly higher genetic stability than Zojka (99.5% and 97.7%, respectively).
- Taxon
- Lonicera caerulea var. kamtschaticaLonicera caerulea var. kamtschaticat0
- Material
- Wojteke0
- Outcome
- genetic stabilitye0
- Direction
- highere0
- Value
- 99.5% and 97.7%e0
- Comparator
- Zojkae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Lonicera caerulea var. kamtschatica”
e0“‘Wojtek’ showed slightly higher genetic stability than ‘Zojka’ (99.5% and 97.7%, respectively)”
Claim · model-written summary
For Zojka plants, the degree of variation was comparable for AFLP and ISSR markers.
- Taxon
- Lonicera caerulea var. kamtschaticaLonicera caerulea var. kamtschaticat0
- Material
- Zojkae0
- Outcome
- degree of variatione0
- Direction
- comparablee0
- Qualifier
- AFLP and ISSR markerse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Lonicera caerulea var. kamtschatica”
e0“For ‘Zojka’ plants, the degree of variation was comparable for AFLP and ISSR markers”
Claim · model-written summary
For Wojtek, no polymorphism was detected with ISSR analysis, in contrast to AFLP analysis.
- Taxon
- Lonicera caerulea var. kamtschaticaLonicera caerulea var. kamtschaticat0
- Material
- Wojteke0
- Outcome
- polymorphisme0
- Qualifier
- no polymorphism was detected using the ISSR analysise0
- Comparator
- the AFLP analysise0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Lonicera caerulea var. kamtschatica”
e0“For ‘Wojtek’, no polymorphism was detected using the ISSR analysis in contrast to the AFLP analysis”
The same questions in other papers
in vitro · growth · Lonicera caerulea (blue honeysuckle)Positive and negative results
5 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
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3 papers more in this group
See all 7 papers →growing medium · growth · Lonicera caerulea (blue honeysuckle)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.