Paper · 2025 · Botanica Pacifica

Effects of pH of a nutrient medium on parameters of growth and rhizogenesis of wild-type blue honeysuckle (Lonicera edulis (Turcz. ex Herder) Turcz. ex Freyn) in in vitro culture

Central Siberian Botanical Garden SB RAS; Anna A. Erst; Irina G. Boyarskikh; et al.

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claims
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verbatim quotes
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citations
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Open access · cc-by-nc

Abstract

Under natural conditions, species of blue honeysuckles grow on soils with a wide range of pH. In this work, for the first time under artificial in vitro conditions, the influence of nutrient medium pH of 3.8 to 8.3 on the viability and growth and development parameters was studied in a species Lonicera edulis from the bluehoneysuckle subsection. It was found that nutrient medium pH does not affect the propagation coefficient of Lonicera edulis; however, more alkaline media caused shortening of its shoots by reducing the length of internodes. Our data suggested that pH affects the rooting of in vitro microshoots of the species under study. Two optima for the rhizogenesis were found: pH 3.8–4.3 (56.5–60.1 % rooting capacity) and pH 6.3–6.8 (53.8–61.1 %). Meanwhile, the height of the shoots and the number of leaves at the rooting stage did not significantly differ among pH values of the medium. Thus, these in vitro culture experiments confirm the tolerance of Lonicera edulis to a wide pH range.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 6

Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Medium pH did not affect propagation coefficient.

Treatment
nutrient medium pHe0
Outcome
propagation coefficiente0
Direction
does not affecte0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lonicera caerulea var. edulis
Lonicera caerulea var. edulis · TaxonAlpsdake · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“nutrient medium pH does not affect the propagation coefficient of Lonicera edulis”

✓ located verbatim in the source · abstract, characters 366–447 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

More alkaline media shortened shoots through shorter internodes.

Treatment
more alkaline mediae0
Outcome
shootse0
Direction
shorteninge0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lonicera caerulea var. edulis
Lonicera caerulea var. edulis · TaxonAlpsdake · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“more alkaline media caused shortening of its shoots by reducing the length of internodes”

✓ located verbatim in the source · abstract, characters 458–546 · show in text ↑
t0

“Lonicera edulis”

✓ located verbatim in the source · title, characters 107–122 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Rooting was optimal at pH 3.8–4.3 with 56.5–60.1 % rooting capacity.

Treatment
pH 3.8–4.3e0
Outcome
rooting capacitye0
Value
56.5–60.1 %e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lonicera caerulea var. edulis
Lonicera caerulea var. edulis · TaxonAlpsdake · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“pH 3.8–4.3 (56.5–60.1 % rooting capacity)”

✓ located verbatim in the source · abstract, characters 691–732 · show in text ↑
t0

“Lonicera edulis”

✓ located verbatim in the source · title, characters 107–122 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Rooting was also optimal at pH 6.3–6.8 with 53.8–61.1 % capacity.

Treatment
pH 6.3–6.8e0
Outcome
rhizogenesise0
Value
53.8–61.1 %e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lonicera caerulea var. edulis
Lonicera caerulea var. edulis · TaxonAlpsdake · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Two optima for the rhizogenesis were found: pH 3.8–4.3 (56.5–60.1 % rooting capacity) and pH 6.3–6.8 (53.8–61.1 %).”

✓ located verbatim in the source · abstract, characters 647–762 · show in text ↑
t0

“Lonicera edulis”

✓ located verbatim in the source · title, characters 107–122 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Shoot height did not differ significantly across pH at rooting.

Treatment
pH values of the mediume0
Outcome
height of the shootse0
Direction
did not significantly differe0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lonicera caerulea var. edulis
Lonicera caerulea var. edulis · TaxonAlpsdake · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the height of the shoots and the number of leaves at the rooting stage did not significantly differ among pH values of the medium”

✓ located verbatim in the source · abstract, characters 774–903 · show in text ↑
t0

“Lonicera edulis”

✓ located verbatim in the source · title, characters 107–122 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Leaf number did not differ significantly across pH at rooting.

Treatment
pH values of the mediume0
Outcome
number of leavese0
Direction
did not significantly differe0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lonicera caerulea var. edulis
Lonicera caerulea var. edulis · TaxonAlpsdake · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the height of the shoots and the number of leaves at the rooting stage did not significantly differ among pH values of the medium”

✓ located verbatim in the source · abstract, characters 774–903 · show in text ↑
t0

“Lonicera edulis”

✓ located verbatim in the source · title, characters 107–122 · show in text ↑

in vitro · growth · Lonicera caerulea (blue honeysuckle)Positive and negative results

7 papers: 6 positive, 1 negative or no effect

6 in the opposite direction

  1. positive
  2. positive
  3. positive

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.