Abstract
BACKGROUND: Understanding the complexity of the vine plant's response to water deficit represents a major challenge for sustainable winegrowing. Regulation of water use requires a coordinated action between scions and rootstocks on which cultivars are generally grafted to cope with phylloxera infestations. In this regard, a genome-wide association study (GWAS) approach was applied on an 'ad hoc' association mapping panel including different Vitis species, in order to dissect the genetic basis of transpiration-related traits and to identify genomic regions of grape rootstocks associated with drought tolerance mechanisms. The panel was genotyped with the GrapeReSeq Illumina 20 K SNP array and SSR markers, and infrared thermography was applied to estimate stomatal conductance values during progressive water deficit. RESULTS: In the association panel the level of genetic diversity was substantially lower for SNPs loci (0.32) than for SSR (0.87). GWAS detected 24 significant marker-trait associations along the various stages of drought-stress experiment and 13 candidate genes with a feasible role in drought response were identified. Gene expression analysis proved that three of these genes (VIT_13s0019g03040, VIT_17s0000g08960, VIT_18s0001g15390) were actually induced by drought stress. Genetic variation of VIT_17s0000g08960 coding for a raffinose synthase was further investigated by resequencing the gene of 85 individuals since a SNP located in the region (chr17_10,497,222_C_T) was significantly associated with stomatal conductance. CONCLUSIONS: Our results represent a step forward towards the dissection of genetic basis that modulate the response to water deprivation in grape rootstocks. The knowledge derived from this study may be useful to exploit genotypic and phenotypic diversity in practical applications and to assist further investigations.
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Claims 5
Claim · model-written summary
Genetic diversity was lower at SNP loci than at SSR loci in the Vitis association panel.
- Outcome
- level of genetic diversitye0
- Direction
- substantially lowere0
- Value
- 0.32e0
- Qualifier
- 0.87e0
- Comparator
- SSRe0
VitisOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the association panel the level of genetic diversity was substantially lower for SNPs loci (0.32) than for SSR (0.87).”
t0“Vitis”
Claim · model-written summary
GWAS found 24 significant marker-trait associations across drought stages.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GWAS detected 24 significant marker-trait associations along the various stages of drought-stress experiment”
t0“Vitis”
Claim · model-written summary
Thirteen candidate drought-response genes were identified.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“13 candidate genes with a feasible role in drought response were identified”
t0“Vitis”
Claim · model-written summary
Three candidate genes were induced by drought stress.
- Treatment
- drought stresse0
- Gene
- VIT_13s0019g03040, VIT_17s0000g08960, VIT_18s0001g15390e0
- Outcome
- Gene expressione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Gene expression analysis proved that three of these genes (VIT_13s0019g03040, VIT_17s0000g08960, VIT_18s0001g15390) were actually induced by drought stress.”
t0“Vitis”
Claim · model-written summary
A SNP in VIT_17s0000g08960 was significantly associated with stomatal conductance.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a SNP located in the region (chr17_10,497,222_C_T) was significantly associated with stomatal conductance”
t0“Vitis”
The same questions in other papers
drought · molecular: expression · VitisPositive and negative results
11 papers in the same direction as this paper · 6 in the opposite direction
- no effect
- positive
- negative
14 papers more in this group
See all 18 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.